CULTURE MEDIA STERILIZATION

Question 1

What does the term “sterile” or “sterilized” mean?

Answer 1

means free of all life, including viruses

Question 2

What is the principal lethal agent for steam sterilization?

Answer 2

Heat

Question 3

T/F:
The lower the temperature, the shorter the time required for sterilization of the media?

Answer 3

False, the higher the temperature, the shorter the time reuqired

Question 4

What is the instrument used to sterilize bacteriological media surgical equipment?

Answer 4

Autoclave

Question 5

How would you determine whether culture media given to you are sterile before you use them?

Answer 5

Broth: should appear transparent and clear

Agar plate/slant : should be no visible growth on surface

Question 6

Define the term sterile

Answer 6

it means clean and sanitized

Question 7

What are the signs of growth in a liquid medium?

Answer 7

Pellicle on surface, sediment on bottom and cloudy (turbid)

Question 8

What piece of equipment was used to sterilize the growth media used in this lab?

Answer 8

autoclave/pressure cooker