Culture Medium Flashcards

(47 cards)

1
Q

Medium for M.tuberculosis

A

Lowenstein Medium

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2
Q

Medium for Neisseria gonorrhea and Neisseria meningitidis

A

Modified Thayer Martin Agar

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3
Q

Medium for gram negative

A

MacConkey Agar

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4
Q

Medium Leptospira interrogans

A

Fletcher’s Media

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5
Q

Medium for Haemophilus influenzae

A

Chocolate Agar

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6
Q

Classify according to CONSISTENCY

Brain Heart Infusion (BHI)

A

Liquid/Nutrient Broth

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7
Q

Classify according to CONSISTENCY

Tryptone Soya Bean/ Soy Bean Casein Digest

A

Liquid/Nutrient Broth

For fungi and anaerobes

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8
Q

Classify according to CONSISTENCY

Thioglycollate

A

Liquid/nutrient broth

Mainly anaerobic, sometimes w microaero and aerobic

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9
Q

Classify according to CONSISTENCY

contains no agar

A

Liquid/ nutrient broth

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10
Q

Classify according to CONSISTENCY

contains 0.5-1%

A

Semi-solid medium

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11
Q

Classify according to CONSISTENCY

Sulfide indole motility (SIM)

A

Semi-solid

Differentiate gram (-) bacteria

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12
Q

Classify according to CONSISTENCY

contains 2-3%

A

Solid

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13
Q

Classify according to CONSISTENCY

Triple sugar iron (TSI)

A

Solid

Differentiate enterics based on the ability to reduce sulfur

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14
Q

Classify according to CONSISTENCY

Mac Conkey Agar

A

Solid

Gram (-), differentiate w/ gram +

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15
Q

Classify according to CONSISTENCY

Chocolate Agar

A

Solid

H. influenzae

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16
Q

Classify according to COMPOSITION

all substances are known
for research purposes

A

Synthetic/Defined

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17
Q

Classify according to COMPOSITION

some substances are unknown
for isolation of bacteria

A

Non-Synthetic/ complex medium

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18
Q

Classify according to COMPOSITION

Nutrient broth

A

Non-Synthetic/ complex medium

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19
Q

Classify according to COMPOSITION

Mac Conkey Agar

A

Non-Synthetic/ complex medium

20
Q

Classify according to COMPOSITION

Tryptone Soya Bean/Soya Bean Cassein Digest

A

Non-Synthetic/ complex medium

21
Q

Classify according to COMPOSITION

isolate obligate intracellular

A

Tissue culture medium

22
Q

Classify according to COMPOSITION

W138

A

Tissue culture medium

human cell kidney culture line

23
Q

Classify according to COMPOSITION

HELA 229

A

Tissue culture medium

chlamydia

24
Q

Classify according to COMPOSITION

Mc Coy Cells

A

Tissue culture medium

chlamydia

25
Classify according to DISTRIBUTION butt, butt-slant, slant, liquid media
Tubed media
26
Classify according to DISTRIBUTION petri dish
Plated media
27
Classify according to USE routinely used in laboratories w/out added supplements
Simple/general purpose
28
Classify according to USE nutrient agar
Simple/general purpose
29
Classify according to USE nutrient broth
Simple/general purpose
30
Classify according to USE tryptone soya bean/ soy bean cassein digest
Simple/general purpose
31
Classify according to USE contain specific nutrients
Enrichment medium
32
Classify according to USE Alkaline peptone water
Enrichment media Vibrio
33
Classify according to USE Selenite F
Enrichment media Salmonella
34
Classify according to USE Buffered charcoal yeast extract
Enrichment media legionella, pneumophila
35
Classify according to USE Thioglycollate
Enrichment media Mainly anaerobic, sometimes w microaero and aerobic
36
Classify according to USE w/ added supplement for fastidious organisms
Enriched media
37
Classify according to USE Blood agar plate
Enriched media nutrient agar + 5% sheep red blood cell
38
Classify according to USE Chocolate agar
Enriched media nutrient agar + powdered Hgb
39
Classify according to USE allows visualization of metabolic differences between species
Differential media
40
Classify according to USE Mac Conkey
Differential media selective differentiates G- from G+ gram - lactose fermenter = pink
41
Classify according to USE Blood agar plate (BAP)
Differential media hemolytic bacteria
42
Classify according to USE Eosin methylene blue
Differential media G+ and G-
43
Classify according to USE hektoen enteric agar
Differential media selective media differentiate salmonella & shigella from other entero
44
Classify according to USE promote growth of desire organism
Selective media
45
Classify according to USE xylose lysine decarboxylase
Selective media shigella & salmonella
46
Classify according to USE bisulfide agar
Selective media salmonella
47
Classify according to USE mannitol salt agar (7.5%)
Selective media for s.aureus mannitol fermenting (lowerarea) - yellow non-fermenting (upper area)