Culture Medium and Culture Method Flashcards
(17 cards)
Need for Culture media
they have to be grown separately on culture media and obtained as pure culture for study.
Types of culture medium
- Liquid medium
- Solid medium
Why solid medium is prefered to study microbes?
- distinct colony morphology
- characteristics - easy to identify
Types of culture media
based on CIO
- Consistency
- Constituents/ ingredients
- Oxygen requirement
Types of consistency
of culture media
cair, keras, separuh keras
- Solid medium
- Liquid medium
- Semi solid medium
examples of each consistency media
example of solid, liquid and semi solid
- Nutrient agar, blood agar ( solid )
- nutrient broth ( liquid )
- motility medium ( semi solid )
Types of ingredients of media
- Simple medium
- Complex medium
- Synthetic / defined medium
- Special medium
Purposes of culture methods
- Isolate bacteria in pure cultures.
- Demonstrate their properties.
- Maintain stock cultures.
- Obtain sufficient growth for preparation of antigens and other tests.
employed depend on the purpose for which they are intend
Culture methods include:
- Streak culture
- Lawn culture
- Stroke culture
- Stab culture
- Pour plate method
- Liquid culture
- Anaerobic culture methods
Purpose of streak culture
Why we do streaking?
Isolation of bacteria in pure culture from clinical specimens.
What equipment used for streak culture?
Platinum wire / Nichrome wire
Uses of Lawn culture
- Bacteriophage typing
- Antibiotic sensitivity typing
- Preparation of bacterial antigens and vaccines.
How Lawn culture are prepared?
By flooding the surface of the plate with a liquid suspension of the bacterium.
Function of stroke culture
Provide a pure growth of bacterium for slide agglutination and other diagnostic tests.
Uses of stab culture
- Demonstration of gelatin liquefaction.
- Oxygen requirements of the bacterium under study.
- Maintenance of stock cultures.
How stab culture prepared?
stab - cucuk
By puncturing a suitable medium ( gelation / glucose agar ) with a long, straight, charged wire.
Uses of pour plate culture?
- Gives an estimate of the viable bacterial count in a suspension.
- Quantitative urine cultures.
