Culturing Microorganisms Flashcards Preview

Biology- cell biology > Culturing Microorganisms > Flashcards

Flashcards in Culturing Microorganisms Deck (11):
1

What is the maximum temperature at which microorganisms in a school lab should be grown ?

25c

2

What type of equipment can be used to transfer microorganisms to an ajarjelly culture medium ?

Inoculating loop

3

Why is it important to sterilise Petri dishes and culture medium before use?

To kill and unwanted microorganisms

4

How can we prevent contamination ?

-heating the inoculating loop over a flame

-tape the lid on

-leave the agar dish upside down

-the petrified dish and culture medium must be sterilised

5

Why is leaving the dish upside down important ?

To stop drops of comdemstation falling on to it

6

Why is tapping the lid on important ?

Stop ,microorganisms from the air getting inn

7

How can bacteria be grown in a lab ?

1.pour hot agar jelly in to a Petri dish

2.use an inoculating loop to transfer the microorganism

3. The microorganisms will multiply

8

What is an inhibition zone ?

The clear area around antibiotic where bacteria have died

9

What does the size of the inhibition zone show ?

How affective the antibiotic is

10

What is the control when investigating the effect of antibiotics ?

A piece of paper soaked in sterile water

This shows the difference in growth

11

What is the equation for calculating inhibition zones ?

Area= pie r2