Cytoskeleton Flashcards

1
Q

Structure of organelles

A

Membranous - Vital function

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2
Q

Particles and inclusions of cell

A

synthesis and storage

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3
Q

Cytoskeleton general

A

support, cell motility, intracellular support

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4
Q

What makes up the cytoskeleton

A

Thin filaments (actin), Microtubules, Intermediate filaments

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5
Q

What makes up thin filaments

A

G-actin and F-actin

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6
Q

Make up of G-Actin

A

Monomeric

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7
Q

Make up of F-actin

A

polymerized, polar (plus and minus ends). About 6 nm diameter

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8
Q

Functions of thin filaments

A

Movement and transport

Structural Support

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9
Q

Movement and transport for thin filaments

A

actin motor - myosin

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10
Q

structural support for functions

A

resists deformation, transmits forces, reinforces plasma membrane

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11
Q

Descriptions of microtubules

A
In all cells except Erythrocytes
Non branching
Hollow cylinders
20-25nm
Polymerized (alpha and beta tubulins)
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12
Q

Structure of microtubules

A

polarity and motors

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13
Q

polarity of microtubules

A

plus end and minus end

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14
Q

plus end of microtubules

A

faster polymerization and located at cell periphery

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15
Q

minus end of microtubules

A

slower polymerization and located at center of cell

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16
Q

motors of microtubules

A

Dyneins and Kinesins

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17
Q

Dyneins

A

move to the minus end

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18
Q

Kinesins

A

move to the plus end

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19
Q

Functions of microtubules

A

Organelle Movement, Mitotic Spindle, Cilia/flagella beating

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20
Q

organelle movement

A

intracellular transport

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21
Q

mitotic spindle

A

chromosome movement

22
Q

Mictrotubules: higher-order structures

A

Axoneme (cilia, flagella)

23
Q

Axoneme (cilia, flagella)

A

9 doublets of microtubules, central pair, dynein arms, structural proteins

24
Q

Centrioles structure

A

(basal bodies)

  1. Hollow cylinders
  2. 160-230nm diameter
  3. 9 triplets of microtubules
25
Q

Centrioles movement

A

replicate during mitosis

migrate to the poles - organize the mitotic spindle

26
Q

MTOC

A

2 centrioles - all cell except neurons and RBCs

27
Q

Intermediate filaments

A

intermediate in thickness, NO polarity, NO motors

28
Q

Major groups of intermediate filaments

A

Lamins, Keratins, Vimentin, Desmin, GFAP, Neurofilament protein (DGLKNV)

29
Q

Location of Lamins

A

Nucleus

30
Q

Location of Keratins

A

Epithelium

31
Q

Location of Vimentin

A

Connective Tissue

32
Q

Location of Desmin

A

Muscle

33
Q

Location of GFAP

A

Glial cells

34
Q

Location of Neurofilament

A

Neurons

35
Q

Functions of intermediate filaments

A
  1. Spread tensile forces
  2. Maintain cell architecture
  3. Act as a cocoon when cell is damaged
  4. Anchor ion channel proteins
  5. Form the nuclear lamina
36
Q

Most common dyes

A

hematoxylin and eosin

37
Q

Nucleus Tissue and Cell Staining

A

Purple/Blue

38
Q

Cytoplasm Tissue and Cell Staining

A

Pink

39
Q

Mitochondria Tissue and Cell Staining

A

Pink

40
Q

RER/Ribosomes Tissue and Cell Staining

A

blue

41
Q

Membranes, Golgi Apparatus Tissue and Cell Staining

A

Not stained

42
Q

Collagen Tissue and Cell Staining

A

Pink

43
Q

Glucosaminoglycans Tissue and Cell Staining

A

Blue

44
Q

what particle does synthesis

A

ribosomes

45
Q

G-actin formation

A

globular so makes big globs

46
Q

what does plus and minus end mean

A

the plus end has faster polymerization than the minus end. It grows faster.

47
Q

how are the cylinders made in microtubules

A

alpha and beta tubulins polymerize head to tail to make cylinders that wrap in a cynlinder formation

48
Q

Dynein and Kinesins are both

A

ATPases

49
Q

Dynein v Kinesin

A

Dynein is bulkier

50
Q

purpose of motor proteins

A

move cargo/organelles

51
Q

what happens if you have a mutation in axoneme proteins

A

huge issues in motility

52
Q

what makes the beating of the cilium

A

dynein arms