Day 6

Question 1

An ultrafiltrate of blood that fills the spaces within and around the central nervous system

Answer 1

Cerebrospinal fluid

Question 2

3 connective tissues that make up the meninges

Answer 2

Dura mater
Arachnoid
Pin mates

Question 3

Where is CSF formed

Answer 3

Found in plexuses which are thin walled capillaries

Question 4

CSF has more ______,_______,_______ but less _______ than plasma

Answer 4

Sodium hydrogen & chloride

Protein

Question 5

How much CSF Is formed daily?

Answer 5

500 ml every 8 hours

Question 6

3 major functions of CSF

Answer 6

Supply nutrients to the nervous tissue
Remove metabolic waste
Cushion the brain and spinal cord against trauma

Question 7

Where on the spinal column is CSF collected from?

Answer 7

In between the 3rd & 4th or 4th & 5th vertebrae

Question 8

Where does each CSF tube tested

Answer 8

Tube 1: chemical and serology testing
Tube 2: microbiology
Tube 3: hematology

Question 9

What is the most frequently performed chemical test on CSF

Answer 9

CSF protein

Question 10

Csf protein blues are higher in what indivi

Answer 10

Infants and older folk

Question 11

What is the most common cause of elevated CSF protein

Answer 11

Meningitis & hemorrhage

Question 12

What test is performed to fraction CSF protein

Answer 12

Electrophoresis

Question 13

The recommended support medium for electrophoresis is ______ followed by staining with _______

Answer 13

Agarose gel

Coomaise blue

Question 14

Characteristics oligolonal bands observed in the gamma region of a CSF protein electrophoresis indicates what disease?

Answer 14

Multiple sclerosis

Question 15

Norma CSF is what percent of the plasma glucose level

Answer 15

60-80%

Question 16

When should a CSF for glucose be collected

Answer 16

2 hours before collecting CSF

Question 17

Why should samples for glucose CSF be collected immediately

Answer 17

Glycolysis occurs rapidly in CSF

Question 18

5 microbiology test that give preliminary diagnosis

Answer 18

Gram stain 
AFB stain 
India ink stain 
Counter immunoelectrophoresis 
Latex agglutination

Question 19

A CSF sample for gram stain analysis should be centrifuges at

Answer 19

1500 RPM at 15 minutes

Question 20

What is the most common cause of false positive reactions on CSF late text agglutination test

Answer 20

Interference of rheumatoid factor‘s

Question 21

How soon should a CSF cell count occur after collection ?why?

Answer 21

30 minutes to prevent RBC and WBC from Lysing

Question 22

When is CSF WBC morphology performed

Answer 22

Counts > 30wbcs/uL

Question 23

A high CSF WBC with a high percentage neutrophils neutrophils is indicative of what disease

Answer 23

Bacterial meningitis

Question 24

An elevated CSF WBC count with a high percentage of lymphocytes and monocytes suggest what disease

Answer 24

Meningitis from viral fungal or parasitic faction

Question 25

Serological CSS examination is typically associated with diagnosis of what disease

Answer 25

Tertiary or neurosyphilis

Question 26

Treatment with_________ In early stages of syphilis has greatly reduce the number of neurosyphilis cases

Answer 26

Penicillin

Question 27

This fluid is a viscous fluid found in joint cavities

Answer 27

Synovial fluid

Question 28

Synovial membrane secrete Mucopolysaccharide containing what

Answer 28

hyaluronic acid and a small amount of protein

Question 29

A needle aspiration to collect synovial fluid is referred to as

Answer 29

arthro-centesis

Question 30

List synovial fluid tube type for each area to be tested

Answer 30

Microbiology sterile heparinized Hematology EDTA Chemistry/ serology no anticoagulated tube

Question 31

Inflammatory immuno logical problems associated with synovial fluid include

Answer 31

Rheumatoid arthritis and lupus

Question 32

Why shouldn’t acetic acid be use for cell counts

Answer 32

It will cause and using clotting and said hypotonic Saline should be used to containing methylene blue

Question 33

What is the two primary crystals found in synovial fluid and the disease is associated with them

Answer 33

``` Monosodium urate (Uric acid) Gout Calcium pyrophosphate Dihydrate CPPD Psyedogout ```

Question 34

Three common chemistry test performed on synovial fluid

Answer 34

Glucose total protein uric acid

Question 35

A needle aspiration to collect pleural fluid is referred to as

Answer 35

Thoracentesis

Question 36

A needle aspiration to collect pericardial fluid is referred to as

Answer 36

Pericardiocentesis

Question 37

I need a laceration to collect peritoneal fluid is referred to as

Answer 37

Paracentesis

Question 38

Explain the difference between serous fluid transudate and exudate

Answer 38

Translates are caused by a mechanical error | Exudates Are caused by inflammatory process and may indicate infection or malignancy

Question 39

Cell counts on serous fluid are done utilizing a

Answer 39

Electronics cell counter

Question 40

What causes the possible milky appearance of plural fluid

Answer 40

Presence of Chylous material and pseudo-Chylous material

Question 41

What chemistry test can differentiate between serous fluid exudates and transudates

Answer 41

Protein Protein <3g/dL transudates >3g/dL exudates