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Histopath, Lec > Decalcification > Flashcards

Decalcification Flashcards

1
Q

done after fixation and before impregnation

A

decalcification

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2
Q

in surface decalcification, dace down on a pad of cotton saturated with _______ hydrochloric acid for approximately one hour

A

10%

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3
Q

in surface decalcification, dace down on a pad of cotton saturated with 10% hydrochloric acid for approximately _________

A

1 hour

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4
Q

four main types of decalcifying agents

A

acids, chelating agents, ion exchange resins and electrical ionization

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5
Q

most widely used agents for routine decalcification of large amounts of bony tissues because they are stable

A

acid decalcifying agents

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6
Q

the most common and fastest decalcifying agent

A

nitric acid

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7
Q

aqueous nitric acid solution 10% time

A

12-24 hours

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8
Q

formol-nitric acid time

A

1-3 days

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9
Q

perenyi’s fluid time

A

2-7 days

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10
Q

phoroglucin-nitric acid time

A

12-24 hours

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11
Q

most rapid decalcifying agent so far

A

phloroglucin-nitric acid

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12
Q

inferior compared to nitric acid in its role

A

hydrochloric acid

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13
Q

von ebner’s fluid time:

A

3-5 days

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14
Q

moderate-acting decalcifying agent

A

formic accid

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15
Q

aqueous formic acid time

A

2-7 days

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16
Q

formic acid-sodium citrate solution time

A

3-14 days

17
Q

trichloroacetic acid time

A

4-8 days

18
Q

it permits good nuclear staining

A

trichloroacetic acid

19
Q

a very weak decalcifying solution suitable only for minute pieces of bone

A

sulfurous acid

20
Q

chromic acid (flemming’s fluid)

A

12 hours

21
Q

can be both used as a fixative and decalcifying agent

A

chromic acid (flemming’s fluid)

22
Q

an environmental toxin

A

chromic acid

23
Q

citric acid-citrate buffer solution

A

6 days

24
Q

ph of citric acid-citrate buffer solution

A

ph 4.5

25
Q

permits excellent nuclear and cytoplasmic staining

A

citric acid-citrate buffer solution

26
Q

most common chelating agent

A

edta

27
Q

optimal ph of edta

A

7-7.6

28
Q

meaning of edta

A

ethylene diamine tetra acetic acid

29
Q

neutral edta time

A

1-3 weeks for small specimens and 6-8 weeks for bones

30
Q

hastens decalcification by removing calcium ions

A

ion exchange resin

31
Q

ion exchange resin time

A

1-14 days

32
Q

a process whereby positively charged calcium ions are attracted to a negative electrode

A

electophoresis

33
Q

hard tissues are placed in the decalcifying agent in a microwave

A

microwave oven decalcification

34
Q

factors influencing the rate of decalcification

A

concentration, fluid access, size and consistency, agitation and temperature

35
Q

optimum temperature

A

18 to 30 degrees celsius

36
Q

water rinsing time for small samples

A

30 mins

37
Q

water rinsing for large specimens

A

1-4 hrs

Histopath, Lec (5 decks)

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