Deck 1 Flashcards
(20 cards)
Principle of PCR
DNA replication
Causative agent of rift valley fever
bunyavirus phlebovirus
susceptible host of FMD
cloven hoofed
what causes ringworm in cattle
Trichophyton verrucosum
what is the one health concept
It is a collaborative, multi-sectorial and trans-disciplinary approach, working at local, regional, national and global levels with the goal of achieving optimal health outcomes recognizing the interconnection between people, animals ,plants and their shared environment.
stages of infectious process -
incubation, prodromal, manifestation, final
transmission of borreliosis
ixodes tick
causative agent of classical swine fever
pestivirus
causative agent of aleutian mink parvovirus
amdovirus
parvovirus
what causes enterotoxaemia in poultry
clostridia
perfringens
What is a calf born with
agammaglobinaemia
how does bacterial culture grow on agar
monolayer, to the
edges
MAT is used to diagnose which bacteria
leptospira
biosafety level 4 are used in which disease?
ebola, small pox
Inhibition haemagglutination test are positive when?
no agglutination, button
Natural foci are described by
geographically & seasonally
swine strepto-meningitis
zoonotic
tetanus are
anaerobic
ELISA main principle
● combining Ab + Ag visualised by an enzyme &chromogen using spectrophotometer
▪ one is labelled with suitable enzyme so there isenzyme activity of the antigen-antibody complex
▪ using suitable substrate that is degraded by theenzyme into coloured products = colour change
▪ often use horseradish peroxidase, betagalatosidase, alkaline phosphatase, lysozyme
▪ detected by spectrophotometer degree of colourdifference between investigated samples andknown control using calibration curve orsubjective = visual
● Indirect ELISA looking for antibody in the serum,plates incubated with antigen, serum added andantibody binds, incubate then wash antiglobulin linkedto enzyme then added and binds to antibody look atcolour reaction
● Sandwich ELISA looking for antigen coat well withspecific antibody, antigen solution added and bound by
capture Ab wash and specific Ab-enzyme labelledantiglobulin added colour reaction
● Competitive ELISA known amount of labelled Agcompetes with investigated amount of non-labelled Agfor Ab binding sites on surface
● Blocking ELISA purified Ag binds to solid phase testserum added, incubated and washed labelled Abagainst Ag added Negative colour reaction = presenceof Ab in serum, positive = no Ab in investigated serum
stain used for mycobacterium
zeihl neelson
