Deck 6 Flashcards
(10 cards)
Which of the following immunoelectrophoresis is for determination of gammopathies in blood serum:
a. Counter immunoelectrophoresis
b. Two dimensional (crossed) immunoelectrophoresis
c. Rocket immunoelectrophoresis
d. Classical immunoelectrophoresis
Classical immunoelectrophoresis
In a direct ELISA for testing of antibody, the intensity of colour measured after adding the substrate in the final step is:
a. Directly proportional to the concentration of antigen in the solid phase
b. Directly proportional to the concentration of antibody in the patient’s serum
c. Directly proportional to the concentration of enzyme-labelled antibody
d. Inversely proportional to the concentration of antibody in the patient’s serum
b. Directly proportional to the concentration of antibody in the patient’s serum
In a sandwich ELISA:
a. The labelled antibody is of identical specificity as the antibody bound to the solid phase
b. The labelled antibody is not of identical specificity as the antibody bound to the solid
phase
c. The antibody is of identical specificity as the antibody found to the solid phase
d. The antibody is of identical specificity as the labelled antibody bound to the solid phase
The labelled antibody is not of identical specificity as the antibody bound to the solid phase
For competitive ELISA is NOT used the step:
a. Antibody is first incubated with a sample containing antigen
b. The antigen-antibody mixture is added to an antigen coated well
c. Enzyme labelled antibody specific to the isotype of the primary antibody is incubated
with an immobilised primary antibody
d. Enzyme labelled antigen is added together with antibody in the sample
Enzyme labelled antibody specific to the isotype of the primary antibody is incubated
In a blocking ELISA:
a. Enzyme labelled antigen is first incubated with an immobilised antibody
b. Enzyme labelled antibody is added after the 1st incubation with the serum containing
antibody
c. Enzyme labelled antibody is added together with the sample
d. Enzyme labelled antibody is immobilised in a solid phase
Enzyme labelled antibody is added after the 1st incubation with the serum containing antibody
Which of the following components is for ELISA:
a. Antibody labelled with fluorescein
b. Antibody labelled with pyocianin
c. Antibody labelled with enzyme
d. Antibody labelled with radioisotope
Antibody labelled with enzyme
Which of the following enzymes is NOT for enzymoimmunoassays:
a. Horseradish peroxidase
b. Alkaline phosphatase
c. Myeloperoxidase
d. Beta-galactosidase
- Myeloperoxidase
- Beta-galactosidase (used in enzyme-linked assays such as beta-galactosidase assays but we don’t cover this)
Which of the following components is NOT substrate for enzymoimmunoassays:
a. TMB
b. BCIP/NBT
c. Fluorescein
d. DAB
Fluorescein
What is conjugate?
Antibody labelled with enzyme
Which of the following components is for IFA:
a. Antibody labelled with fluorochrome
b. Antibody labelled with peroxidase
c. Antibody labelled with alkaline phosphatase
d. Antibody labelled with radioisotope
Antibody labelled with fluorochrome
