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Flashcards in Determining blood cell parameters Deck (10):
1

How is red cell count estimated?

- Dilute the blood 200x and place a layer 0.1mm thick on a special ruled haemocytometer slide
- To dilute the blood, 5 microlitres is added to 995 microlitres of HAYEM’S solution using a capillary pipette
- By counting those in a known area, the number or red cells in 1 litre of blood can be estimated
- E.g. counting the amount in 5 medium squares (volume=0.02mm^3) , and mutiplying by 10^10 to get per litre

2

How is haematocrit measured?

Haematocrit (Hct) is the percentage of the volume of a sample of blood occupied by the red cells
- This can be measured using a centrifuge
- The blood will separate into layers
- The ratio of the length of the red cell column to the total length is haematocrit

3

How is Hb concentration measured?

Haemoglobin concentration in the anticoagulated blood sample is measured using the HemoCue Hb 201+ system,

4

How is MCV calculated?
Units?

Hct / RBC count x 100
fl (10^-15 l)

5

How is MCH calculated?
Units?

(Hb x 10) / RBC count

pg (10-12 g)

6

How is MCHC calculated?
Units?

(Hb x 100) / Hct

g/dl

7

Normal MCV?

80 – 100 fl

8

Normal MCH?

26 – 34 pg

9

Normal MCHC?

32 - 36 g/dl

10

Normal leukocyte count?

3.5 - 7.5 *10^9 /L