Diagnostics Flashcards
(20 cards)
specific gravity range
1.05-1.24
Physaloptera
1.238
Taenia
1.225
Giardia
1.18
Trichuris vulpis
1.145
Toxocara cati
1.1005
Toxocara canis
1.090
Ancylostoma sp.
1.056
simple gravitational approach
not great
advise against
modified double centrifugal flotation
strain, wash, decanter, float
sugar solution
eggs
dilution techniques
get rid of debris
McMaster’s Technique & Stoll Technique
McMaster’s Technique
use to get rid of debris/artifact
measures mg/g
Acid Fast Staining
not used in small animals
good for scouring calves because they have so many cysts so it gives smaller sample size
immunofluorescence assays
test of choice
good for small animals
baermann technique
larvae
sedimentation
eggs too heavy to float
e.g. trematode eggs
trichomonad testing
flagellated organisms (e.g. protozoans)
Knott’s Test
heartworm
detects microfilaria
other heartworm diagnostics
ELISA
lateral flow devices
squash preps
clearing an organism
favorite technique
transparency