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PARASITOLOGY QUIZ 2 LABORATORY > DIFFERENT TECHNIQUES > Flashcards

DIFFERENT TECHNIQUES Flashcards

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1
Q

ARTIFACTS THAT MAYBE MISTAKEN FOR PARASITE

A

PLANT CELL
VEGETABLE FIBER
MEAT FIBER
VEGETABLE HAIR\
POLLEN GRAINS
VEGETABLE SPIRALIS
STARCH GRANULES
BUBBLES

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2
Q

IN STOOL EXAMINATION THERE ARE 2 TYPES

A

QUANTITATIVE TECHNIQUE \
QUALITATIVE TECHNIQUE

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3
Q

IN QUALITATIVE TECHNIQUE THERE ARE 2 TYPES

A

UNCONCENTRATION TECHNIQUE
CONCENTRATION TECHNIQUE

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4
Q

UNCONCENTRATION TECHNIQUE UNDER

A

SIMPLE SMEAR

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5
Q

2 TYPES OF CONCENTRATION TECHNIQUE

A

FLOTATION TECHNIQUE
SEDIMENTATION TECHNIQUE

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6
Q

UNDER OF FLOTATION TECHNIQUE

A

Brine flotation
Sugar flotation
Zinc- sulfate flotation

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7
Q

UNDER OF SEDIMENTATION TECHNIQUE

A

Simple sedimentation
Centrifugal sedimentation
Formalin ether concentration technique

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8
Q

are hexogonal bipyramidal structures localized in the primary granules of the cytoplasm of eosinophils and basophils

A

Charcot Leyden crystal

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9
Q

their presence, along with eosinophilic infiltrate, is an indirect evidence of parasitic infestation, particulary with

A

Toxocara, capilliriasis, ascariasis or fasciola

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10
Q

are formed from the?

A

breakdown/ disintegration of eosinophils

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11
Q

and maybe seen in the stool or sputum of patients with

A

parasitic diseases

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12
Q

indicate inflammation

A

PMNs

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13
Q

indicate immune response to a parasitic infection

A

eosinophils

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14
Q

ulcerations or bleeding

A

red blood cells

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15
Q

phagocytes, active macropahage can be mistaken for amebic trophozoites

A

macropahages

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16
Q

released with the disintegration of eosinophils , may indicate presence of hypertensitivity or arasitic ingfections, especially amebiasis

A

Charcot Leyden crystals

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17
Q

Fungal spores from blank, yeast and yeast-like fungi

A

Candida spp

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18
Q

sodium hydroxide + blank of stool specimen

A

4g

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19
Q

eggs counted x blank x factor = number of eggs per gram

A

200

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20
Q

factor will depend on stool consistency

A

hard (x1)
mushy (x2)
loose (x3)
liquid (x4)

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21
Q

do not use this technique in

A

liquid stool

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22
Q

used to isolate

A

schistosoma spp

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23
Q

look for the bkank (first ova larva)

A

Maracidium

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24
Q

provide the ability to detect small numberd of parasites that might not be dtected using direct wet preparation

A

concentration techniques

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25
to aggregate parasites present into a small volume of the sample and to remove as much debris
purpose
26
to aggregate parasites present into a small volume of the sample and to remove as much debris
purpose
27
protozoan blank do not usually survive the procedure
trophozoites
28
concentration can separate protozoan cysts and helminth eggs from a larger amount of stool usually what gram in amount? based on differences in specific gravity
1 gram
29
2 types of concentration methods
sedementation flotation
30
parasites are concentrated in the sediment of the tube following centrifugation and the sediment is examined microscopically
sedimentation
31
parasites are less dense than the solutions used and , during centrifugation they float to the surface
flotation
32
most widely used
formalinethyl acetate sedimentation
33
procedure in formalin ethyl acetate sedimentation
1. ethyl acetate is added to a saline washed formalin fixed sample and the tube is then centrifuged 2. The tube is then decanted and the sediment is examined in a wet prep, unstained (i.e with saline) and with iodine
34
parasite are blank than the solution and settle in the sediment of the tube, whereas fecal debris is usually lighter and rises to the upper layer of the test tube
heavier
35
advatange of formalin ethyl acetate sedimentation
provides good recovery of most parasites and iiis easy to perform
36
disadvantage of formalin ethyl acetate sedimentation
the preparation contains more fecal debris than a flotation technique and is more challenging to the microspist
37
kato thick
qualitative examination
38
kato katz
quantitaive examination
39
debris is heavy and sinks to the blank of the test tube and potential parasites are lighter and float toward the top of the tube
bottom
40
advantage of zinc sulfate flotation method
more fecal debris is removed yields a cleaner preparations easier for microscopic examination
41
disadvantage of zinc sulfate flotation method
some helminth eggs are very dense and will not float, therfore some parasites will be missed
42
in surface fil what parasite
light
43
in the sediment what parasite
heavy
44
stools are directly mixed with ?
brine
45
it is need to centrifuge in brine solution or not?
no need
46
it is lost cost and simple in brine solution or not?
lost cost and simple
47
not useful for blank
operculated eggs
48
is like hookworm and schistosma become badly shrunken in this technique
helmith eggs
49
solution preserved with phenol
boiled sugar
50
best for the recovery of mainly cryptosporidiu, cyclospora, and cystisospora
coccidian oocysts
51
used to isolate
schitodoms spp (mansoni)
52
look for the ( first stage larva)
miracidium
53
final procedure in the ova and parasite examination
permanent stained smear
54
microscope slide that contains a fixed sample that has been dried and subsequently stained
permanent stained smear
54
microscope slide that contains a fixed sample that has been dried and subsequently stained
permanent stained smear
55
Helminth eggs or larvae are best dtetcted and identified using a ? technique
concentration
56
prepared slide of see-through thickness made from a PVA- preservced sample for permanent staining
thinly
57
slides are reviewed under
oil immersion (100x)
58
? are reviewed before the slide can be considered ?
300 fields negative
59
most widely used permanent stain
wheatley trichrome stain
60
reveals excellent morphology of the intestinal protozoa
Iron hematoxylin stain
61
important permanent stain procedure for the detection of the oocycts of ?, as well as those of ? and ?
cryptosporidium isospora cyclospora
62
modified acid fast stain incorporates a ? step
carbol
63
this allows for the detection of acid fast parasites in addition to the other protozoa normally recovered using the iron hematoxylin stain
modified acid fast stain
64
kits with monoclonal antibody detects antigens in specimens
alternative tests
65
preserved stool
unacceptable
66
purpose of stool culture to differentiate ? and ?
larvahookworm strongyloides
67
true or false stool should not refrigerated because some species failed to develop when exposed to cold temperature
true
68
or test tube culture method
harada mori
69
filariform larvae generally moves downward against capillary movement collected water
hookworm
70
larvae move upward accumulate in upper part of filter paper
strongyloides stercoralis
71
positive stool are ixed with moistened soil or granulated charcoal larvae harvested in Baermann procedure
capro culture
72
protozoan that may be cultured
entamoeba species acanthamoeba species entamoeba histolytica naegleria fowleri toxoplasma gondii trichomonas vaginalis
73
culture media for protozoan culture
Boeck Drbohrlv media Cleveland Collier McQuay Diphasic charcoal media Balamuth (nutrient fluid) Diamond media- diphasic media Novy Mcheal Nicole medi/ NNN for Leishmania spp and trypanosoma spp

PARASITOLOGY QUIZ 2 LABORATORY (1 decks)

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