Diffusion and Active Transport overview Flashcards
Protein synthesis, DNA replication, Phospholipid Bilayer, diffusion, osmosis, active transport
Describe the role of DNA in the cell
DNA contains genetic information that encodes the sequence of amino acids in a protein and serves as a permanent store of genetic information.
Explain how RNA functions in the cell
RNA acts as an intermediary, carrying instructions from DNA in the nucleus to the ribosome for protein synthesis. It is temporary and does not last long.
Compare the structure of DNA and RNA
DNA: Double helix, contains deoxyribose, bases adenine (A), guanine (G), cytosine (C), and thymine (T).
RNA: Single strand, contains ribose, bases adenine (A), guanine (G), cytosine (C), and uracil (U).
Explain the purpose of mRNA in protein synthesis
mRNA acts as a messenger carrying genetic information from DNA to the ribosome, where it is used to assemble a protein.
Describe the process of DNA replication
DNA replication involves unwinding by helicase, priming by primase, synthesis by DNA polymerase, proofreading, and joining of DNA fragments to produce two identical DNA molecules.
Explain the role of helicase in DNA replication
Helicase unwinds the DNA double helix by breaking hydrogen bonds between complementary base pairs.
What is the function of DNA polymerase in replication?
DNA polymerase adds complementary nucleotides to the template strand and has proofreading ability to correct mistakes.
Describe the process of transcription
In transcription, a DNA strand is unwound by helicase, and mRNA is synthesized using one of the DNA strands as a template. The mRNA then exits the nucleus through nuclear pores.
What happens during translation?
During translation, mRNA attaches to a ribosome, tRNA brings amino acids, and they are assembled into a polypeptide chain. The polypeptide then folds into a functional protein.
Explain the role of the Golgi Apparatus in protein processing
The Golgi apparatus modifies, processes, and packages proteins into vesicles for transport outside the cell via exocytosis.
Describe the process of exocytosis
Exocytosis involves substances being enclosed in a vesicle within the cell. The vesicle moves through the cytoplasm, fuses with the cell membrane, and releases its contents outside the cell.
What is required for vesicle fusion in exocytosis?
Calcium ions (Ca²⁺) are required for the vesicle to fuse with the cell membrane during exocytosis.
Explain the structure of the phospholipid bilayer
The phospholipid bilayer consists of hydrophilic phosphate heads and hydrophobic fatty acid tails. The bilayer is flexible and semi-permeable, regulating the movement of substances.
Describe the role of choleserol in the phospholipid bilayer
Cholesterol stabilizes membrane fluidity by preventing the bilayer from becoming too rigid or too fluid.
define diffusion
Diffusion is the net movement of solutes from an area of high concentration to an area of low concentration, until equilibrium is reached.
describe the process of osmosis
Osmosis is the movement of water through a semipermeable membrane from an area of lower solute concentration (hypotonic) to higher solute concentration (hypertonic).
explain what happens to a cell in a hypotonic solution
In a hypotonic solution, water enters the cell, which may cause it to swell or burst.
what occurs to a cell in a hypertonic solution?
In a hypertonic solution, water leaves the cell, causing it to shrink.
explain active transport
Active transport is the movement of molecules against their concentration gradient (from low to high concentration) using energy in the form of ATP.
describe the process of active transport
Active transport involves a molecule binding to a transport protein, ATP hydrolysis provides energy, the protein changes shape to move the molecule across the membrane, and the protein resets after the molecule is released.
what is the role of sodium-potassium pumps in active transport?
Sodium-potassium pumps move sodium (Na⁺) out of the cell and potassium (K⁺) into the cell, maintaining proper ion balance and cellular functions.
explain facilitated diffusion
Facilitated diffusion is the transport of molecules across the cell membrane via specific carrier or channel proteins, moving down the concentration gradient without requiring ATP.
describe the difference between channel proteins and carrier proteins in facilitated diffusion
Channel proteins form pores for specific ions or molecules to pass through, while carrier proteins bind to molecules, change shape, and transport them across the membrane.
explain the role of DNA in protein synthesis and health
DNA stores genetic information and provides instructions for protein synthesis, which is essential for cellular function, growth, and repair in the body.