Digestion Lab Flashcards
Enzyme
a protein catalyst that increases the rate of specific chemical reactions
substrate
the molecules that combine with the active sites of an enzyme and that are converted to products by catalysis of enzyme
Factors that might affect enzyme activity, WHY?
temperature, pH, enzyme concentration, substrate concentration
IT AFFECTS THE RATE AT WHICH ENZYMATIC REACTIONS PROCEED
temperature
the rate of an enzyme-catalyzed reaction increases as the temperature is raised
pH
Any change in pH above or below the Optimum will quickly cause a decrease in the rate of reaction
enzyme and substrate concentration
increases the rate of reaction b/c more substrate molecules will be colliding with enzyme molecules, so forming more product
Test Tube 1
starch, distilled(DI) water
Test Tube 2
starch, amylase
Test Tube 3
starch, amylase, HCL
Test Tube 4
starch, amylase, NaOH
Test Tube 5
starch, boiled amylase
Results of test tube 1
starch test was positive
no maltose present (negative) - no enzyme to digest the starch in this tube
results of test tube 2
both tests were positive - amylase digested the starch, producing the maltose
results of test tube 3
environment in this tube was acidic (not optimal for amylase function - amylase may have been denatured)
THEREFORE some digestion may have occurred (positive maltose test) but not as much as was seen in tube 2
results of test tube 4
environment in this tube was basic (which is not optimal for amylase function - amylase may have been denatured)
THEREFORE some digestion may have occurred (positive maltose test) but not as much as seen in tube 2
