DNA Flashcards Preview

Higher Human Bio - Unit 1 (Cell Biology) > DNA > Flashcards

Flashcards in DNA Deck (15)
Loading flashcards...
1

A double helix is

made up of 2 strands of DNA

2

The 4 DNA bases are

Guanine, Cytosine, Adenine, Thymine

3

Adenine pairs with

Thymine

4

Guanine pairs with

Cytosine

5

Deoxyribose sugar can be found at the

3' end

6

Phosphate can be found at the

5' end

7

What bonds break after DNA unwinds?

Hydrogen bonds

8

What is needed to start replication

A primer

9

What enzyme joins fragments of DNA together?

Ligase

10

What enzyme adds the complementary nucleotides during replication?

DNA Polymerase

11

What end can nucleotides be added to?

The 3' end

12

Why is DNA initially heated to 92-98oC during PCR?

To break the hydrogen bonds and separate the strands

13

What doe PCR make?

Amplified copies of DNA

14

During PCR, What temperature is the DNA cooled to and why?

It is cooled to 50-65oC to allow primers to bind

15

What can PCR be used for?

To solve crimes, settle paternity suits and diagnose genetic disorders