Dna 16-18 Flashcards

(33 cards)

1
Q

Dna synthesize from

A

5’ to 3’

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2
Q

What is the term topoisomerase

A

It is when the DNa has many forms

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3
Q

Helicase function

A

Unzip dna for initial insertion

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4
Q

Exonuclease function

A

To clip out the primers

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5
Q

Primers are made from what

A

Rna

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6
Q

What connects the gaps between dna fragments

A

Ligase

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7
Q

Whats a dna cap

A

Telomere (shows the end of the strand)

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8
Q

Po3 function

A

Enlongation

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9
Q

Po1 function

A

Clean primers, and fill in the gaps

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10
Q

Common function between po1 and po3

A

Can both proofread

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11
Q

Types of mutation

A

Substitution, deletion and insertion

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12
Q

What checks for mutation

A

MuTL (checks the methylation of the dna)

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13
Q

What removes the mutation

A

MUTH (removes and ligase would connect the back)

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14
Q

Muts function

A

Proofread again

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15
Q

Uc effect on dna

A

They can cause the cross link of the thymine. Remove by phosphorylation

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16
Q

Whats translesion

A

Forcing the completion eventhough theres an error cause it cannot be fixed

17
Q

Po4 and 5 function

A

Po4 would overlook a damage whereas po5 would not

18
Q

Whats a double base strand repair

A

If the strand is homo= make a new strand
If the strand is hetero= ligase fix

19
Q

function of tata

A

dictate the replication direction

20
Q

steps to regulation of gene

A

sequence -> promoter -> tata

21
Q

positive control eg

22
Q

negative control

23
Q

what are opérons

A

a cluster of gene that is controlled via on or off switch. responsible for many related expressions

24
Q

name bacteria opérons

A

lac z, lac y, lac a

25
lac opérons should normally be
off
26
function of operator
the in or off switch
27
how does lac op. works
once lactose is present in the system, binds to the repressor to remove them. the synthesis runs and proteins are made to digest the lactose
28
can lac be positive
yes. according tô the amount of lac in the lac1
29
how many trp proteins are there
5
30
trp is normally on or off
on
31
transcription factor pathway
protein binds promoter then to enchaine then the synthesis can take place
32
what makes trp turns off
triptrophan
33
what happened when there not triptrophan
operator works (always on) and triptrophan is made