dna Flashcards

(39 cards)

1
Q

nucleotide

A

monomer of nucleic acid

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2
Q

What are the components of a nucleotide?

A

Pentose sugar, phosphate plus organic base which contains nitrogen.

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3
Q

pentose sugar in dna vs rna

A

dna=deoxyribose
rna=ribose

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4
Q

nucleotide bases are either

A

purine(two rings)
pyrimidine(one ring)

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5
Q

pyrimidine bases

A

thymine
cytosine
uracil(rna)

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6
Q

purine bases

A

adenine
guanine

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7
Q

how are nucleotide bases linked to other molecules

A

by condensation reactions to form polymers

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8
Q

dna structure

A

composed of 2 chains linked by base pairs which are complementary
forms a double helix which is only 2nm in diameter

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9
Q

in a chromosome how long is dna

A

85mm long

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10
Q

dna antiparallel

A

one srand goes from 5’ to 3’, another goes from 3’-5’, this makes the double helix

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11
Q

functions of dna

A

replication- has two complementary strands; if two separate 2 more form and each is a template
protein synthesis-sequence of bases represents dna info and determines aa sequence

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12
Q

cell division , daughter cell

dna replication allows

A

accurate copying for cell division, each daughter cell has an exact copy of genetic info

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13
Q

semi conservative replication

A

dna is unwound and nucleotides are separated by dna helicase
nucleotide opposite complementary bases on template, hydrogen bonds form and nucleotides link by dna polymerase
each replica is a hybrid of parent and new strand

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14
Q

meselson and stahl

A

ecoli cultured in 15n and amino acids providing evidence for semi conservative replication

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15
Q

meselson and stahl first gen

A

dna produced 14n and 15n as it divided once
band produced of a mixture of 14 and 15, but rose a bit because of this

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16
Q

meselson and stahl second gen

A

two bands produced as the relative abundance of the 14n increases

17
Q

meselson and stahl third and fourth gen

A

lighter band gets stronger
hybrid band gets fainter

18
Q

DNA is a store of

A

genes, genetic info coded into bases

19
Q

how does amino acid sequence determine characteristics of an organism

A

sequences direct amino acids which determine proteins and enzymes which direct reactions which make up characteristics of an organism

20
Q

dna is a triplet code

A

it comes in 3s which code foe specific amino acids

21
Q

how many dna codes are possible

22
Q

dna code is non overlapping

A

each base only is in one triplet, and you read it separately

23
Q

dna code is degenerate

A

more than one triplet can encode each amino acid

24
Q

dna code is unambiguous

A

same codon does not code for 2+ amino acids

25
dna code is universal
all organisms have the same triplet codes
26
dna code is punctuated
there are 3 codons not for amino acids but for STOP CODONS
27
codons vs triplet of bases
codons is in mrna triplet of bases is in dna
28
exons
regions of dna that code for proteins/are expressed
29
introns
regions of non coding dna that contain blocks of repeated nucleotides
30
mrna function
transcribes instructions in dna and carries these to ribosomes
31
trna
clover shape, provides linkage between mrna and amino acids transfers amino acids to ribosomes
32
most prokaryotes are continuous
they have no introns/non coding sequences their entire gene is transcribed and translated
33
one gene one polypeptide argument
one gene can have a variety of polypeptides because various exons produced by splicing can be cut and stuck in many ways
34
gene
portion of dna that codes for a polypeptide chain
35
post translational modification
modification of a polypeptide- can be modified by combination with non proteins
36
carbohydrates lipids phosphates
glycoproteins lipoproteins phospho-proteins
37
example of post translational modification
haemoglobin has secondary, tertiary and quaternary structure
38
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