DNA Flashcards

(42 cards)

1
Q

how many strands in dna serve as a template for transcription

A

1 parental strand

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2
Q

describe deoxyribonucleotude

A

double stranded, dna, deoxyribose sugar, ATGC, phosphates

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3
Q

double helix, what does it provide

A

2 linked strands that wind around each other. provides a measure of protection

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4
Q

semi conservative

A

half of the original dna appears in the replicated dna

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5
Q

anti parallel

A

strands run in opposite directions

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6
Q

describe ribonucleotide

A

single stranded, ribose sugars, AUGC, RNA, phosphates

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7
Q

where is mRNA made, what does it do

A

made in the nucleus,
- carries genetic message from DNA in the nucleus
- to ribosome in the cytoplasm for protein synthesis

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8
Q

replication fork

A

Y shaped site where DNA is separated and synthesized

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9
Q

3’ and 5’ ends

A

DNA polymerase can only add nucleotides in the 5’ to 3’ direction (moves along the template strand in the 3’ to 5’ direction)

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10
Q

template strand

A

parental strand

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11
Q

leading strand

A

built continuously 5’ to 3’

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12
Q

lagging strand

A

synthesized in fragments

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13
Q

helicase

A

unwinds

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14
Q

polymerase

A

adds nucelotides

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15
Q

ligase

A

links fragments

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16
Q

ribosome

A
  • construction site
    translates mrna into polypeptide chains to assemble proteins.
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17
Q

trna

A

transfers amino acids to ribosme to make proteins

18
Q

what is a cells source of supply of amino acids?

A

diet (essential amino acids)

19
Q

codon

A

triplet of nitrogen bases on mrna. codes for amino acids

20
Q

anticodon

A

trna that carries specific amino acid to complimentary base pair with codon

21
Q

what happens to transfer rna after it drops off its amino acod

A

leaves the translation “e” site, recharged to find another amino acid to deliver

22
Q

silent substitution

23
Q

missense substitution

A

change in codon

24
Q

nonsense substitution

25
addition/deletion
frameshift: codons after mutation change
26
what are proteins, what is it made through
chains of amino acids that do jobs in our bodies made through translation and protein synthesis
27
Where is translation?
in the ribosome
28
initiation (translation)
Ribosome binds to the MRNA, entering the a site. tRNA to the start codon
29
elongation (translation)
- ribosome reads mrna codons - trna brings amino acids to the ribosome in the P site, exits with the E site - ribose forms a polypeptide chain
30
termination (translation)
ribosome reaches stop codon, chain released with hydrolysis from the release factor
31
separation (replication)
dna helicase unwinds double helix, breaking H bonds. - single stranded binding proteins prevent re joining
32
complementary base pairing (replication)
dna polymerase adds nucleotides. - rna primase begins replication by making primers. once primers are in place polymerase adds nucleotides
33
joining (replication)
dna ligase links fragments with phosphodiester bonds
34
site of replication for prokaryotes
no nucleus, replication in cytoplasm
35
transcription
copying genetic info from DNA to mRNA
36
initiation (transcription)
rna polymerase binds in the promoter region (TAA box), exposing template strand and attaches
37
elongation (transcription)
mRNA symthesized
38
termination (transcription)
rna polymerase and new rna are released, dna is reformed
39
how many genes are transcribed with transcription
one
40
rna polymerase
enzyme that does transcription
41
______ codons reduce errors
diverse
42
rna splicing
removes introns (pre rna) and reconnects exons