DNA extraction methods Flashcards
1
Q
Solid Extraction
A
for low levels of DNA in sample (swabs or touch DNA)
2
Q
Epithelial extraction
A
high source samples (direct amount of skin/ bodily fluids)
3
Q
Differential extraction
A
for samples containing sperm –> washes are done to remove female cells
4
Q
Extraction negative
A
Used to identify contamination –> a blank sample is run
5
Q
Quantification of DNA
A
determining how much is present, using dyes or probes to track it
use inter calating dyes (fit into grooves w/in structure)
6
Q
types of quantifiers
A
- quantifiler
- Pico green - not human specific but quick and easy
- HY - indicates how much male DNA is present
