DNA Profiling

Question 1

Gel electrophoresis

Answer 1

A type of chromatography that separates nucleic acid fragments or proteins by size using electric current

Question 2

VNTR (variable number tandem repeats).

Answer 2

A location in DNA where a short nucleotide sequence is organized as a tandem repeat with variations in lengths between individuals.

Question 3

Protease

Answer 3

An enzyme used to hydrolyse proteins

Question 4

DNA purification

Answer 4

DNA extraction is a method to purify DNA by using physical and/or chemical methods from a sample separating DNA from cell membranes, proteins, and other cellular components.

Question 5

Lysing

Answer 5

Cell lysis or cellular disruption is a method in which the outer boundary or cell membrane is broken down or destroyed in order to release inter-cellular materials such as DNA, RNA, protein or organelles from a cell.

Question 6

Ethanol

Answer 6

An alcohol used in DNA extraction which allows the precipitation of the DNA

Question 7

DNA profiling

Answer 7

A technique used to determine the pattens in the non-coding DNA of an individual. It involves five main stages: DNA extraction: Digestion; digestion; separation of DNA fragments; hybridisation; and observation

Question 8

PCR

Answer 8

An in vitro technique used to rapidly amplify fragments of DNA.

Question 9

DNA sequencing

Answer 9

Determining the entire nucleotide base sequence of an organism