DNA/PROTEIN SYNTHESIS Flashcards

(38 cards)

1
Q

what are the components of DNA

A

made up of nucleotides

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2
Q

what are the components of RNA

A

nucleotides

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2
Q

what is the function of RNA

A

decode messages from DNA

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3
Q

what enzymes are involved in DNA replication

A

helicase, DNA polymerase

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3
Q

what did Rosalin Franklin discover? what methods did she use

A

double helix in DNA, used x-rays

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4
Q

what is transcription

A
  • RNA molecules are produced by copying part of a nucleotide sequence of DNA into a complementary sequence in RNA
  • during transcription, RNA polymerase binds to DNA and separates the DNA strands
  • RNA polymerase then uses one strand of DNA as a template from which nucleotides are assembled into a strand of DNA
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4
Q

What are Chargaff’s rules?

A
  • the percentages of guanine (G) and cytosine (C) bases are almost equal in any sample of DNA
  • the percentages of adenine (A) and thymine (T) bases are almost equal in any sample of DNA
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4
Q

what can an error in DNA replication cause?

A

genetic mutation and disorders

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4
Q

Name three differences between DNA and RNA.

A
  • in RNA, the sugar is ribose rather than deoxyribose
  • RNA is single stranded
  • different bases: RNA: A-U, C-G, (Uracil)
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5
Q

What are the rules of base pairing?

A

A-T, C-G

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6
Q

TCGGCAC

A

AGCCTG

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7
Q

Where are proteins produced?

A

cytoplasm

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7
Q

ATGCCCGGAT

A

TACGGGCCTA

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8
Q

what is the replication fork

A

site where separation and replication occur

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8
Q

What are the functions of mRNA?

A

mRNA/messenger RNA: encodes amino acid sequence in polypeptide, decodes the information in DNA
carries copies of instructions for assembling amino acids into proteins

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8
Q

What is the name for each nucleotide triplet that codes for amino acids?

9
Q

What are the 3 types of RNA?

A

mRNA, tRNA, rRNA

9
Q

DNA replication:

A
  • S phase of interphase
  • before a cell divides, it replicates its DNA in a process called replication
  • during DNA replication, the DNA molecule separates into two strands
  • each strand of the double helix of DNA serves as a template for the new strand
9
Q

transcription and translation.

A

Now DNA is used by cells
Now the instructions in DNA are read and followed
DNA is a recipe for protein (sequence of amino acids)
Messages in DNA are decoded by RNA

9
Q

what are the functions of tRNA

A

tRNA/transfer RNA: brings amino acids to ribosomes during translation
during protein construction, tRNA transfers each amino acid to the ribosome

9
Q

function of DNA polymerase

A
  • principle enzyme involved in DNA replication
  • joins individual nucleotides together produce a DNA molecule and then “proofreads” each new strand of DNA
10
Q

INCOMPLETE DOMINANCE

A

Neither allele is dominant over the other
Heterozygous genotypes show an in-between phenotype
Heterozygotes will have a blend of traits
AKA intermediate inheritance
Example: snapdragon flower color
Homozygous: RR = red
Homozygous: rr = white
Heterozygous: Rr = pink

10
Q

what enzyme is involved in transcription

A

RNA polymerase

11
Q

components of nucleotide

A

monomer of nucleic acids, composed of five-carbon sugar (deoxyribose) a phosphate group, and a nitrogenous base.

12
how replication occurs
- dna is carried out by enzymes that "unzip" a molecule of DNA (helicase) - hydrogen bonds between the base pairs are broken and the two strands of DNA unwind
13
what is translation
Translation is the decoding of an mRNA message into a polypeptide chain Translation takes place on ribosomes During translation, the cell uses information from messenger RNA to produce proteins The ribosome binds new tRNA molecules and amino acids as it moves along the mRNA The process continues until the ribosome reaches a stop codon extra: Each tRNA molecule carries only one kind of amino acid In addition to amino acids, each tRNA molecule has three unpaired bases These bases, called anticodon, are complementary to one mRNA codon process: BEGINS when a mRNA molecule attaches to a ribosome As each codon of the mRNA molecules moves through the ribosome, the proper amino acid is brought into the ribosome by tRNA In the ribosome, the amino acid is transferred to the growing polypeptide chain
13
where does transcription occur
nucleus
14
where does translation occur
on ribosomes in the cytoplasm
15
mendelian dominance
One allele is dominant over the other Heterozygotes show the dominant phenotype Example: pea plant flower color: PP = purple Pp = purple pp = white
16
POLYGENIC INHERITANCE
Whenever multiple genes affect a characteristic, the variation of phenotypes within a population can become even greater When teo or more genes affect a single character, it is called Polygenic inheritance Examples: Eye color (8 different genes) Height Skin color (over 150 genes) Hair color
16
codominance
Neither allele is dominant over the other Heterozygous genotypes show both alleles Example: blood type in humans
16
BLOOD TYPES IN HUMANS
Letters in blood types relate to proteins attached to red blood cells Type A blood has A antigens (proteins that tell your immune system that you are you) on RBC → blood/plasma contains Anti-B antibodies Type B blood has B antigens on RBC → blood/plasma contains Anti-A antibodies Type AB blood has A and B antigens on RBC → no anti A or Anti B antibodies Type O blood has neither A or B antigens (Naked) → blood/plasma contains anti-A and anti-B antibodies Type A blood can be noted as I^A I^A or I^A i Type B blood can be noted as I^B I^B or I^B i Type AB can be noted as I^A I^B
17
GREGOR JOHANN MENDEL (1822-1884)
Father of genetics What is genetics: Field of biology devoted to understanding how characteristics are transmitted from parents to offspring Mendel observed 7 characteristics A characteristic is a heritable feature, such as flower color Each characteristic occurred in 2 contrasting traits A trait is a genetically determined variant of a characteristic, such as yellow flower color Mendel’s experiments The P generation → F1 generation → F2 generation Mendel hypothesis Something within the pea plants controlled the characteristics Called these controls factors (now known as alleles) Each trait was inherited by means of a separate factor A pair of alleles/factors controlled each trait
18
law of independent assortment
Multiple factors separate independently of one another during the formation of gametes Traits produced by dominant factors do not necessarily appear together Factors for individual characteristics are not connected
18
law of segregation
A pair of factors is segregated/separated during the formation of gametes Paired factors separate during the formation of reproductive cells Each gamete receives one factor of each pair When two gametes combine during fertilization, the offspring will have two factors for each characteristic
19
HOW MANY ALLELES DO WE GET FROM OUR PARENTS
During meiosis, gametes receive ONE chromosome from each homologous pair of chromosomes Thus, when the gametes combine in fertilization, the offspring receives from EACH parent ONE allele for a given trait
19
SEX DETERMINATION
Like homologous chromosomes, sex chromosomes also pair during meiosis I. as meiosis proceeds, the paired chromosomes separate and move to different cells. When an egg (always carries an X) is fertilized by a sperm cell (X and Y), the offspring will be either male or female
20
sex linked traits
Sex linked traits - a trait that is coded for by an allele located on a sex chromosome An X chromosome is much larger than a Y chromosome so there are MORE X-linked than Y-Linked traits Most X-linked traits have no homologous counterpart on the Y chromosome. In humans, the Y only contains the gene for “maleness” Because males only have one X chromosome, a male who carries a recessive allele on the X will exhibit that X-linked trait Example: colorblindness is an X-linked recessive trait, therefore most people who are colorblind are males