DNA Replication Flashcards
DNA Replication is
Semiconservative replication
Each dna is half
Half old and half new
Weakest bonds that can be seperated easily
Hydrogen bonds
Goal of replication
To replicate, duplicate, recreate DNA
Where does replication happen
Nucleus
Uncoils and unwinds dna
Topoisomerase
Unzips DNA, realing the hydrogen binds between nitrogenous bases
Helkcase
Stabilizes the single strands
Single Stranded Binding Protein
Signals polymerase where to go
Primase
Initiating enzyme
Primase
Makes a new strand to partner the left strand
Dna polymerase
5’ to 3’
Leading strand
3’ to 5’
Lagging strand
In what prime direction does DNA polymerase builds bases
5’ to 3’
Seals the break in the new DNA strand
Ligase
Okazaki fragments are only found on
Lagging strand
Cells need to make identical copies of their genetic information for
Growth and repair
When does replication happen
Before cell division
Process of DNA Replication
- Unwinding
- Base Pairings
- Joinings
Unwinding
- DNA Helicase breaks the H bond between nucleotides
- Divides DNA into two single strand
Base Pairings
- DNA Polymerase adds complementary base pairs
- Proofreads
Joining
- Ligase seals any breaks
Repairs the dna dtrand when damaged by harmful radiation or toxic chemicals
DNA Polymerase and Ligase
other enzymes remove and replace incorrectly paired nucleotides that have resulted from replication errors
Mismatch repair
