DNA replication

Question 1

prokaryotic initiator protein

Answer 1

DnaA

Question 2

OriC

Answer 2

Single origin of replication in bacteria

Question 3

Composition of sequence adjacent to OriC

Answer 3

AT rich. Base pairing is less stable than GC so DNA can open more easily to start the replication bubble.

Question 4

DnaB

Answer 4

helicase in prokaryotes (DnaC helps)

Question 5

DnaG

Answer 5

primase in prokaryotes

Question 6

DNA polymerase I function

Answer 6

DNA polymerase I function

Question 7

DNA polymerase III function

Answer 7

Replicative chain elongation

Question 8

Exonuclease activity of DNA pol I

Answer 8

5’ to 3’ (unique to pol I) and 3’ to 5’

Question 9

Which prokaryotic DNA polymerase has higher processivity?

Answer 9

DNA polymerase III

Question 10

Beta subunit of DNA pol III

Answer 10

sliding clamp with low internal affinity for DNA. Responsible for high processivity of DNA pol III.

Question 11

Gamma complex of DNA pol III

Answer 11

clamp loader

Question 12

Core enzyme of DNA pol III

Answer 12

Theta, epsilon, and alpha subunits

Question 13

𝛂 subunit of DNA pol III

Answer 13

polymerase

Question 14

ε subunit of DNA pol III

Answer 14

proofreader with 3’ exonuclease ability

Question 15

ter sites

Answer 15

Indicate termination region. Binding site for TUR protein

Question 16

ek DNA pol for leading strand replication

Answer 16

DNA pol 𝛆

Question 17

ek DNA pol for lagging strand replication

Answer 17

DNA pol 𝛅

Question 18

ek DNA pol for repair

Answer 18

DNA pol β

Question 19

ek DNA pol with high processivity with PCNA

Answer 19

DNA pol 𝛅

Question 20

DNA polymerases highly sensitive to aphidicolin

Answer 20

alpha, delta, and epsilon

Question 21

PCNA

Answer 21

equivalent to the beta subunit of polymerase III–sliding clamp shaped like a ring.

Question 22

Origin of replication in yeast

Answer 22

ARS - autonomous replicating sequences

Question 23

Helicase in yeast

Answer 23

MCM - mini chromosome maintenance proteins

Question 24

Stabilize ssDNA in yeast

Answer 24

RPA - replication protein a

Question 25

Synthesizes DNA in mitochondria

Answer 25

DNA polymerase γ

Question 26

Removes RNA primers in yeast

Answer 26

Rnase H and Fen1 (flap endonuclease 1)

Question 27

What does the pre-replication complex include?

Answer 27

ORC, inactive helicase (MCM), other proteins

Question 28

pk DNA polymerase involved in SOS repair

Answer 28

DNA pol II, IV, and V

Question 29

What does the holoenzyme complex of DNA pol III consist of?

Answer 29

2x (α, ε, θ, β, and τ) and 1x (δ, δ’, γ, χ and ψ)

Question 30

Which ek DNA polymerases lack 3’ exonuclease activity?

Answer 30

Alpha and beta. These cannot proofread.

Question 31

What kind of topoisomerase separates interlinking strand of replicated DNA in prokaryotes.

Answer 31

Topoisomerase type II

Question 32

Licensing

Answer 32

Assembly of pre-replication complex

Question 33

Anticipation

Answer 33

Worsening of a disease with successive generations

Question 34

Protein component of telomerase

Answer 34

hTERT - human telomerase reverse transcriptase

Question 35

RNA component of telomerase

Answer 35

hTR - human telomerase RNA

Question 36

How is the 3’ overhang stabilized?

Answer 36

Loop structure with proteins called TTAGGG repeat binding factors (TRF-1 and TRF-2)