DNA Technology

Question 0

What do restriction enzyme do for bacteria?

Answer 0

Provide a defense mechanism for viruses

Question 1

What are short tandem repeats?

Answer 1

Short sequences of DNA that are repeated numerous times

Question 2

Use of restriction enzymes?

Answer 2

To cut dna in smaller fragments at specific nucleotide sequences

Question 3

Polymerase chain reaction?

Answer 3

Allows us to use vast quantities of DNA to trace samples

Question 4

Ingredients in a polymerase chain reaction

Answer 4

Template dna, dna primers, deoxynucleotide triphosphates, polymerase with a buffer

Question 5

What is a sticky end

Answer 5

A DNA fragment that has ends with exposed nucleotide bases at each en

Question 6

Steps for splicing DNA

Answer 6

1. extract plasmid DNA from bacteria
2. cut the plasmid DNA using restriction enzymes
3. hydrogen bond the plasmid DNA to non plasmid DNA frags
4. use ligase to seal plasmid DNA to non plasmid DNA
5. transform bacteria with q recombinant DNA molecule

Question 7

Steps of pcr

Answer 7

1. the mixture is heated to a high temperature to denature target DNA
2. the primers hybridize the DNA
3. DNA polymerase extends the primers to make a copy of the target DNA

Question 8

What separates restriction fragments from one another

Answer 8

Gel electrophoresis

Question 9

How does a gel separate out fragments

Answer 9

According to their size

Question 10

Reproductive cloning

Answer 10

A nucleus is inserted into an egg then into the female

Question 11

Therapeutic cloning

Answer 11

Normal DNA is inserted into a plasmid then human so the DNa works correctly

Question 12

DNA cloning

Answer 12

A period of DNA is cut from the specimen and inserted into a plasmid DNA then into a bacteria

Question 13

What are RFLP’s

Answer 13

Restriction format length polymorphism a, a DNA sequence that can be detected when the DNA is cleaves by restriction enzymes