DOC CHE 1 Flashcards
are specialized proteins
produced by the immune system in response
to the presence of foreign substances called
antigens.
aB
are molecules (often
proteins or carbohydrates) that are recognized
by the immune system as foreign or non-self.
Ag
measures the direct interaction
between Ag and Ab in vitro
Primary binding tests
(ELISA, IFA, RIA)
measures the consequences
of interaction between Ag and Ab in vitro
Secondary binding tests
(Agglutination,
CFT, Precipitation, Neutralization tests)
measures Ag and Ab
interactions in vivo (in body)
- Tertiary binding tests
two main ways of performing ELISA
Double antibody technique, to detect antigen
2. Indirect technique, to detect and assay antibody.
how many uL in CHW
10 ul then 3 drops buffer
how many uL for BP testing
20 Ul diluted sa buffer
qPCR uses a fluorescent Fluorescent dye
dye that only fluoresces
when bound to
double
stranded DNA (dsDNA).
The cycle at which fluorescence is first
observable above background is called
Ct for cycle threshold
In a PCR, the amount of target sequence
doubles every cycle. This means that if a
reaction reaches Ct one cycle earlier than
another reaction, it is expected to have
started
with twice as much target sequence.
If there is a two-cycle difference, you expect a
____ fold difference in starting DNA
concentration. A three-cycle difference, ____ fold and so on.
4
8
why use nuclease free water /
Nucleases such as DNAses and RNAses are enzymes that cleave DNA and RNA strands into smaller fragments, and if present can actively degrade DNA in samples, DNA extracts, reagents, PCR products and primers.
Brings the capillaries of the allanotic
into direct contact with the shell
membrane allowing calcium
reabsorrption
chorion
Embryonic respiratory organ
allantois
