DREADDs

Question 1

what does DREADDs stand for?

Answer 1

Designer receptors exclusively activated by designer drugs

Question 2

what is the activation form of DREADD?

Answer 2

hM3Dq

Question 3

what is the inhibitory form of DREADD?

Answer 3

hM4Di

Question 4

describe steps for AAV vector with DREADD to infect a cell

Answer 4

AAV attaches to a cell membrane, undergoes receptor-mediated endocytosis and endosomal trafficking
AAV then escapes from late endosome or lysosome and translocates to the nucleus.
virus uncoats in the nucleus.
dsDNA is produced via host cells polymerase
fate of AAV depends on presence of a helper virus, such as HSV.

Question 5

what promoter is needed for neuron expression of DREADD and for general expression of DREADD

Answer 5

Neuron: hSyn
General: CAG

Question 6

three factors that determine expression of DREADD

Answer 6

promoter, serotype of AAV, cre-dependent

Question 7

BBB penetrant DREADD ligands

Answer 7

JHU 37152 and JHU 37160

Question 8

Why CNO and not clozapine?

Answer 8

CNO has low potency at endogenous receptors

Question 9

“Rapid, reversible activation of ____ neurons drives feeding behaviour in mice”

Answer 9

agouti-related protein

Question 10

other DREADD ligands

Answer 10

compound 21, JHU37152, JHU 37160, perlapine