E1 Micrometry and Hemocytometer

Question 1

Objective of Micrometry

Answer 1

To measure size of specimen under microscope

Question 2

Micrometry makes use of what 2 key tools (aside from the microscope)

Answer 2

Ocular and stage micrometer

Question 3

This is usually 10um per division, goes with magnification. Acts as the standard

Answer 3

Stage Micrometer

Question 4

This is on the eyepiece and doesn’t change

Answer 4

Ocular Micrometer

Question 5

How will you calibrate for the ocular micrometer using the stage micrometer

Answer 5

Dimensional analysis
Eye micrometer divs/Stage micrometer divs

Question 6

What is the function of Hemocytometer

Answer 6

To count cells

Question 7

How will you calculate the number of cells?

Answer 7

DF10^4cells/[large] square

Question 8

In the central square method, how will you estimate cell count in the [large] square?

Answer 8

Get 5 random [small] squares and count the cells in them before multiplying by 5.

Question 9

True or False, Clumped cells should be counted individually

Answer 9

False, count as 1, to avoid clumping disturb the cells

Question 10

True or false, you should count all cells even those touching the lines per square

Answer 10

False, you will set an arbitrary guide for which sides you will not count, between top or bottom and left or right.