ECM

Question 1

IFs form polymers of 10nm diameter between what? microfilaments and microtubules

Answer 1

microfilaments and microtubules

Question 2

IF Encoded in genome by how many?

Answer 2

70 different genes (5 families by sequence homology).

Question 3

IF: Expressed in what kind of manner?

Answer 3

tissue specific manner.

Question 4

Is IFs are polarized or not?

Answer 4

NOT polarized

Question 5

structure of IF proteins

Answer 5

conserved rod domain, flanked by nonhelical N-and C-terminal domains. N and C domains different, so dimer is polarized.

Question 6

IF: structure of Rod domain

Answer 6

roughly 310 amino acids, and is a-helical, coiled-coil structure

Question 7

IF: a-helix in coiled-coil domain

Answer 7

characterized by heptad repeat sequence, with hydrophobic residues usually at positions 1 and 4. Helices of two proteins pack together, mediated by interactions between these hydrophobic side chains.

Question 8

IF dimers associate with other dimers in what fashion?

Answer 8

in anti-parallel fashion, forming a tetramer.

Question 9

4 roles of the basal lamina

Answer 9

1. tissue organization into distict compartments
2. tisseu function
3. tissue repair
4. cell migration

Question 10

what is basal lamina made of?

Answer 10

1. Type 4 collagen
2. laminins
3. perlecan
4. nidogen(or entactin)