Energetics and Enzymes Flashcards Preview

MCD - Metabolism > Energetics and Enzymes > Flashcards

Flashcards in Energetics and Enzymes Deck (14):
1

Concept of free energy

The amount of energy within a molecule that could perform useful work at a constant temperature.

2

When is a reaction spontaneous?

When delta-G is negative

3

Why does glucose + fructose --> sucrose not take place spontaneously until coupled with ATP's reaction to form ADP?

Glucose + fructose = sucrose is +23KJ/mol - not spontaneous.
ATP -> ADP = -31KJ/mol = spontaneous
When coupled, overall delta G is 23-31= -8KJ/mol - spontaneous

4

How do enzymes act as catalysts?

Increase rate
Provide alternative reaction route with a lower activation energy for molecules to reach their transition state.

5

Graph of rate of reaction against increasing substrate concentration

Increases steadily then plateaus past point of saturation

6

Graph of enzyme activity against temperature

Slowly increases to peak at optimum temperature, steep decrease beyond this point

7

Graph of enzyme activity against pH

Increases from 0 steadily to peak at optimum pH, decreases steadily as pH increases away from optimum pH

8

Definition of a coenzyme

A non-protein molecule that is necessary for an enzyme to function but itself only functions after binding to a protein.

9

Role of NAD in reactions catalysed by dehydrogenases?

Catalyses the dehydrogenation of substrates by readily accepting a hydrogen atom and two electrons.

10

How can enzyme activity be measured using spectrophotometry?

Rate of reaction can be recorded by measuring and comparing absorbance at regular intervals throughout the reaction

11

What is Vmax?

The maximum rate once enzyme is saturated with substrate

12

What is Km?

The substrate concentration that corresponds to the rate of reaction equal to Vmax/2

13

Effect of a competitive inhibitor on Vmax and Km

Increases Km
Vmax remains the same

14

Effect of a non-competitive inhibitor on Vmax and Km

Km remains the same
Decreases Vmax