Energy/TCA

Question 1

4 Products from Pyruvate

Answer 1

Lactate (Cori cycle)
Alanine (Cahill cycle, for N transport from muscles back to liver)
OAA
Acetyl-CoA

Question 2

5 Cofactors for Pyruvate Dehydrogenase Complex

Answer 2

TLC For Noone:
Thiamine (B1, Pyrophosphate, TPP)
Lipoic Acid
CoA (B5, pantothenic acid)
FAD (B2, riboflavin)
NAD (B3, niacin)

Question 3

Arsenic

Answer 3

Inhibits lipoic acid, get gastroenteritis symptoms (nauseau/vomiting) with GARLIC BREATH

Question 4

3 Important Enzymes for TCA Cycle

Answer 4

Citrate synthase - incorporates Acetyl-CoA so makes Citrate
Isocitrate dehydrogenase (RL)
alpha-KG dehydrogenase (regulated by same 5 cofactors as PDH)

Question 5

3 Complex I Inhibitors

Answer 5

Amytal (barbiturate)
Rotenone (fish poison)
MPP (methyl-phenylpyridinium) - metabolite from MPTP from trying to make opiates

Question 6

Inhibitor of Complex II

Answer 6

Antimycin A

Question 7

2 Complex IV Inhibitors

Answer 7

CN, CO

Question 8

Complex V (ATP Synthase) Inhibitor

Answer 8

Oligomycin

Question 9

3 Uncoupling Agents (& what happens)

Answer 9

Increase permeability of membrane so H+ gradient decreases and O2 consumption increases. Produces heat
Aspirin
Thermogenin in brown fat
2,4-Dinitrophenol (2,4-DNP) wood preservative abused for weight loss