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BACTE LAB > ENTEROBACTERIACEAE > Flashcards

ENTEROBACTERIACEAE Flashcards

1
Q

RAPID LACTOSE FERMENTERS

A

Escherichia coli
Klebsiella spp
Enterobacter spp.

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2
Q

NON-LACTOSE FERMENTERS

A

Salmonella spp except for S. arizonae
Shigella except S. sonnei
Proteus
Providencia
Morganella
Edwardsiella

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3
Q

LATE LACTOSE FERMENTERS

A

Salmonella arizonae
Shigella sonnei
Serratia spp
Hafnia spp
Yersinia spp
Citrobacter

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4
Q

Triple Sugar Iron (TSI) pH indicator

A

Phenol Red

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5
Q

TSI H2s indicator

A

Sodium thiosulfate is reduced to Sulfur Dioxide and Hydrogen Sulfide which reacts with Ferrous Sulfate
to produce black ferric sulfide

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6
Q

To determine if a g- bacilli
decarboxylates or
deaminates lysine and forms
H2S

A

Lysine Iron Agar (LIA)

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7
Q

If oxidative deamination of lysine
occurs

A

it will form a burgundy (red) color on
the slant in the presence of ferric ammonium
citrate and flavin mononucleotide.

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8
Q

If deamination does not occur

A

LIA slant remains purple

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9
Q

When glucose is fermented, the butt of
the medium becomes

A

acidic (yellow)

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10
Q

If the organism produces lysine
decarboxylase

A

cadaverine (purple) is
formed

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11
Q

If the decarboxylase is not produced,
the butt remains

A

acidic (yellow)

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12
Q

check organism (+) for
deamination

A

Slunt part

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13
Q

check organism (+) for
deamination

A

Slunt part

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14
Q

check organism (+) for
decarboxylation

A

Butt part

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15
Q

Organisms that involved deamination

A

Proteus, Morganella and Providencia

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16
Q

if the bacteria can produce sulfide
it will result of blackening

A

SULFIDE (H2S+)

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17
Q

INDOLE Result

A

Pink ring formation (+)
need Kovac’s reagent/Ehrlich’s reagent

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18
Q

POSITIVE IN INDOLE

A

Proteus vulgaris

19
Q

NEGATIVE IN INDOLE

A

Proteus mirabilis

20
Q

 SIM would have heavy turbidity.
 Can also form a brush like formation.
 If the organism shows this it is
automatically

A

MOTILITY

21
Q

 To determine if the m/o is capable
of utilizing citrate as its sole source
of carbon.

A

CITRATE UTILIZATION TEST

22
Q

POSITIVE REACTION FOR CITRATE

A

BLUE

23
Q

NEGATIVE REACTION FOR CITRATE

A

GREEN CITRATE

24
Q

Citrate useful in identification of lactose
fermenters

A

E, K, E

25
Q

Positive organisms in Citrate Utilization Test

A

Klebsiella and
Enterobacter

26
Q

Negative organisms in Citrate Utilization Test

A

Escherichia coli

27
Q

To identify the rapid urease
producers and weak urease
producer

A

Urease Test

28
Q

POSITIVE RESULT FOR UREASE

A

Pink/Red formation

29
Q

NEGATIVE RESULT FOR UREASE

A

Yellow color

30
Q

BROTH USE IN UREASE TEST

A

Christensen’s agar/
Stuart broth

31
Q

Rapid Urease producers within 2-4 hrs

A

Proteus, Providencia and Morganella

32
Q

Weak Urease producers more than 4 hrs

A

Kleibsiella pneumoniae and
Enterobacter spp, Yersinia spp, Serratia
and Citrobacter

33
Q

Sugars in TSI

A

Lactose= 10
Sucrose=10
Glucose=1

34
Q

pH indicator of MacConkey agar

A

neutral red

35
Q

only media for detecting gram negative
in hospital settings
 Selective for gram negative organisms
 Carbohydrate present: Lactose
 Differential media: it differentiate
lactose fermenters and non lactose
fermenter

A

MacConkey agar

36
Q

If organism is lactose fermenters

A

pink
colonies

37
Q

ENTEROBACTERIACEAE
GENERAL CHARACTERISTICS

A

Gram (-)
 Facultative anaerobic
 Glucose fermenters
 Oxidase (-)

38
Q

Motile with peritrichous flagella
(except

A

Klebsiella, Shigella, Yersinia

39
Q

Non-encapsulated (except

A

Klebsiella
and Enterobacter)

40
Q

Catalase (+) (except

A

Salmonella
dysenteriae)

41
Q

Nitrate reducers (except

A

Erwinia and
Pantoea agglomerans)

42
Q

Often with Gas production (except

A

Shigella, Salmonella, Proteus and
Providencia)

43
Q

Commensal flora in the intestinal
tract (except

A

Salmonella, Shigella and Yersinia

44
Q

Nonhemolytic except

A

In BAP: Escherichia coli- beta hemolysis

BACTE LAB (4 decks)

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