Entomology and Taphonomy Flashcards

(27 cards)

1
Q

What is forensic entomology?

A

the study of insects as it relates to criminal investigations, commonly used to estimate the PMI

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2
Q

In addition to the PMI, what else can insect activity indicate?

A

abuse/peri-mortem wounds and drug abuse

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3
Q

What is taphonomy?

A

the study of changes after death, often encompasses environmental factors

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4
Q

What is characteristic of an ante-mortem fracture?

A

Moist and greasy, moisture will be lost over time after death

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5
Q

What is characteristic of a post-mortem fracture?

A

Clean, brittle (bone is dry), often parallel to the cross section of the long-axis of the bone

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6
Q

What are the 4 stages of decay?

A
  • Death
  • Fresh
  • Bloat (more detail in another question)
  • Active Decay (more detail in another question)
  • Also the dry stage - bones
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7
Q

How does the bloat stage of decay occur?

A
  • bloating of abdominal cavity due to active proliferation of bacteria
  • Increased odor
  • marbling of skin, green-purple U shape
  • skin slippage on extremities (glove look)
  • perforation of abdominal cavity due to insect activity, gasses released
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8
Q

How does the active decay stage occur?

A
  • enhanced insect activity
  • deflation of body cavity due to perforation
  • wet appearance
  • very strong odor
  • total loss of 50% body mass
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9
Q

how are insects used to find buried remains?

A
  • survey soil for insect activity
  • blow fly can lay eggs in loose soil and larvae migrate to body
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10
Q

what are some field techniques to ID a grave?

A
  • roughly rectangular disturbance, visible months later
  • pile of soil and disturbed vegetation
  • grave surface forms a mound (mixed with air and body)
  • grave eventually becomes a depression with rain and time
  • plant succession
  • soil takes years to become uniform with the surroundings
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11
Q

What other cases utilize entomology?

A
  • livestock food contamination
  • Abuse and neglect (bugs in diapers, beg sore bugs)
  • can have toxins or chemicals in stomachs (indicate drug use)
  • tell if a corpse was moved
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12
Q

What was the first documented use of entomology?

A

Sun Tzu’s Washing Away of Wrongs 13th century. Details a murder that was solved by flies colonizing a sickle

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13
Q

When, and who, disproved the theory of spontaneous generation?

A

1626 Francesco Redi, using covered and uncovered jars with raw meat

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14
Q

How did Matthieu Orfilia contribute to entomology?

A

Mid-1800s, pathologist, systematized 30 types of insects and anthropods that feed on and oviposit in humans

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15
Q

How was entomology used in the Steven Truscott case?

A
  • Original PMI was wrong
  • proven that the species of fly does not lay eggs at night, thus, Truscott had an alibi for the night
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16
Q

What is the insect succession pattern in the first stage of decay: fresh?

A
  • necrophagous species
    1. Caliphordae (blow and green/blue bottle fly)
    2. Sarcophagidae fly
    3. Silphidin beetle (carrion-eating)
17
Q

What is the insect succession pattern in the second stage of decay: bloat?

A
  • predatory species (eat other insects)
    1. histeridae beetles
    2. Staphylinidae
18
Q

What is the insect succession pattern in the third stage of decay: active decay?

A
  • Hymenoptera
  • Parasitic Wasps
19
Q

What is the insect succession pattern in the fourth stage of decay: dry?

A
  • species that make use of dry remains
  • spiders, springtails, some mites, round worms
20
Q

What is the main challenge when estimating PMI?

A

Temperature as it effects growth rate - must record temp at scene

21
Q

What is the life cycle of a blowfly like?

A
  • Day 1: eggs
  • Day 2: larvae emerge (instar)
  • Day 3-7: larvae grow (pre-pupae)
  • Day 8-9: pre-pupation form hard cocoon shell, develop adult features
  • Two weeks: full adult emerges
  • Warmer temps could expedite this
22
Q

How are maggots aged?

A
  • Based on spiracles (breathing parts)
  • 1st Instar have 1, 2nd have 2, 3rd have 3
23
Q

How is blowfly colonization used to estimate PMI?

A
  • estimation of age is better than succession
  • temperature dependent growth (below 15 degrees they go dormant)
  • different species develop at different rates
  • useful 1-30 days post-mortem
24
Q

What are the 4 main problems with using entomology to estimate PMI?

A
  • Maggot mass: generate heat, increase growth rate
  • geography: species grow differently in different locations
  • Buried/clothed remains: may limit ability to colonize immediately
  • Larval crowding: competition for resources, affects growth rate
25
How are larval species identified?
- must be reared to adult for confirmation - leads to delayed results - sometimes molecular id is used (another question)
26
How are insects collected from a scene?
- collect a bunch, kill them and preserve them asap, sort into instar - collect live ones, rear to adult - collect from all 'wet' orifices and cracks (clothing folds)
27
How is molecular id used in insect species?
- uses 300bp of subunit 1 of cytochrome oxidase in mtDNA or nuclear region of the ITS2 - interspecies variation should be greater than 3% to positively ID