Environmental microbiotechnology Flashcards

(31 cards)

1
Q

what are organohalogens

A

class of organic compounds that contain at least one halogen (fluorine [F], chlorine [Cl], bromine [Br], or iodine [I]) bonded to carbon.

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2
Q

what are DTDs

A

pesticides - bad

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3
Q

what are PCBs

A

industrial products/chemicals - bad

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4
Q

an example of a bed chemical in water that is removed

A

phenols

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5
Q

what else is removed from water in waste water treatment

A

remove high BOD compounds - biological oxygen demand - measures the amount of organic carbons that bacteria can oxidise

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6
Q

what is nitrification

A

oxidation of ammonia to nitrite, then nitrite to nitrate

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7
Q

what is denitrification

A

organic matter is oxidised to produce nitrogen gas - harmless to the atmosphere
nitrate reduced to nitrogen

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8
Q

what is the Anammox process

A

nitrification and denitrification where you start with ammonia and end with nitrogen (giving out water)
nitrite can be used to oxidise ammonium - you don’t need oxygen - use the intermediate

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9
Q

what is kuenenia stuttgartiensis

A

a bacterium that contains an anammoxosome

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10
Q

what happens in the anammoxosome

A

anaerobic oxidation of nitrite

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11
Q

what are the advantages of the anammox process

A

don’t need oxygen
88% less co2 produced
90% reduction in operation costs

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12
Q

what are xenobiotics

A

synthetic compounds with no obvious counterparts in the natural world - we make them - foreign - introduced

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13
Q

3 examples of xenobiotics

A

pesticides, fertilisers, PAHs - from combustion processes

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14
Q

what is biomagnification

A

when a substance accumulates in the fats of larger animals as it moves through the food chain eg DDD in ducks from the water x1000 conc

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15
Q

why is a mixture of microos better at degrading organic compounds rather than a pure culture

A

all have different degradation properties
one could partially degrade, then another could take over
some might be more sensitive than others

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16
Q

many xenobiotics are mixed with heavy metals, what does this mean for the degradation

A

slows down the degradation process as gram +ive and -ive are sensitive to toxic metals

17
Q

what is bioremediation

A

organisms used to eliminate environmental contamination eg. oil spills

18
Q

what does in situ mean

A

relies on microorganisms indigenous to the site of contamination - organisms are natural - found there anyay

19
Q

what are common limiting factors to the microorganisms doing the degrading

A

nitrogen, phosphorus, oxygen - fertilisers added to encourage degradation

20
Q

what is Ab

A

a bacterium that preferably uses alkane hydrocarbons as substrates - likes exclusively on alkanes

21
Q

name the alkanes

A

methane
ethane
propane
butane

22
Q

what are pseudomonas

A

gram -ive bacteria

abundant in soil, very metabolically versatile, inc. hydrocarbons

23
Q

what do pseudomonas contain

A

a catabolic plasmid that is self transmissable

24
Q

what is the benzine ring

A

a major building block in nature, found in lignin

25
what are AHs and an example
Aromatic Hydrocarbons eg benzene
26
what are AH's degraded to
catechol
27
what are the two pathways that can be induced depending on different benzene and catechol derivatives and where do the products feed into
ortho-cleavage pathway - Acetyl CoA + succinate meta-cleavage pathway - Acetaldehyde + pyruvic acid feed into central metabolism - TCA cycle (Krebs cycle)
28
where would anaerobic degradation have to occur
in waterlogged places/ rubbish tips
29
what is toluene and how is it degraded
an aromatic hydrocarbon | oxidised to Co2 using iron as the e- acceptor
30
what technique is used to recover metals from their ore
bioleaching - solubilisation by acid production
31
what technique is used for recovery of precious metals
bioaccumulation - microos accumulate them due to -ive surface charge