Enxymes During Replication

Question 1

Unzips double strands at Ori Site

Answer 1

Helicase

Question 2

Relives TENSION and STRESS on unzipped DNA (Prevents from supercoiling)

Answer 2

Topoisomerase

Question 3

Prevents unzipped strands from re-attaching

Answer 3

Single Strand Binding Protein

Question 4

Provides RNA primers to initiate replication. Supplies RNA primers in the Okazaki fragments

Answer 4

Primase

Question 5

Synthesize majority of DNA in the 5 to 3 direction. Main enzyme for replication in bacteria

Answer 5

DNA polymerase III

Question 6

DNA repair and exonuclease activity. Removes RNA primers

Answer 6

DNA polymerase I

Question 7

Acts as a glue. Connects Okazaki fragments not fused by polymerase I

Answer 7

Ligase