ENZYME CALCULATIONS Flashcards
1
Q
Equation for normal graph of substrate (x) against velocity (y)
A
this is called the michaeles menton equation
V0= Vmax [S] / KM + [S]
2
Q
Linear equation for the lineweaver- burk plot
A
1/ V0 = Km/ Vmax x 1/[S] + 1/Vmax
this can be put into
y = mx + c
the x= 1/ [S]
3
Q
What is the equation for the slope on the lineweaver burk plot?
A
Km / Vmax
4
Q
What is the x intercept on the lineweaver- burk plot?
A
-1/km
5
Q
What is the y intercept on the lineweaver- burk plot?
A
1/ Vmax
6
Q
What is the x axis on the lineweaver - burk plot?
A
1/ [S]
7
Q
what is the y axis on the lineweaver- burk plot?
A
1/ V0
