Enzyme Cell and Membrane Test Flashcards

1
Q

What is a catalyst?

A

Substance that speeds reaction

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2
Q

Enzyme shape determines which reaction it catalyzes (same both sides)

A

Enzyme shape determines which reaction it catalyzes

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3
Q

Why are enzymes considered to be biological catalysts?

A

speed reaction, works under living organism conditions

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4
Q

Describe the model of induced fit

A

Enzyme changes shape/confirmation so enzyme can bind to substrate

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5
Q

Enzymes substrate complex and what happens at an active site

A

Enzyme binds to one or more substrate,
converts to one or more product, Catalyze reaction repeats and binds

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6
Q

How does an active site differ from an allosteric site

A

Active site: location for catalyse reaction, hydrophobic
Allosteric site: changes shape in enzyme

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7
Q

Cofactor

A

-non protein group
-binds to enzyme
-metals
-production of energy function requires cofactor

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8
Q

Coenzyme

A

-Shuttle molecules from one enzyme to another
-NAD is electron carrier of pathways
-Organic molecule acts as cofactor for enzyme

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9
Q

methods of enzyme inhibition

A

-Lowers catalyzes rate
-Decrease activity
-Fits in active site
-Block access to normal substrate thats why activity is slow

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10
Q

Competitive inhibition

A

-Concentration is high enough it stops completely
-They compete for a spot in the active site

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11
Q

Non competitive

A

-don’t compete for a spot
-bind to a location other than the active site
-Can’t bind easily because they change shape

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12
Q

Allosteric sites

A

-behave like non competitive reversible inhibitors.
-Bind to site thats not active site, changes shape

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13
Q

Allosteric regulation

A

-protein affected by a molecule binding to another site that’s not the active site

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14
Q

Role of feedback inhibition

A

-Product of allosteric regulation
-Prevents resources being wasted

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15
Q

Feedback inhibition examples

A

-thermostat connected to heater
-a cell does not need to break down glucose if there’s enough energy

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16
Q

Animal cells vs plant cells

A

-Animal cells have a centrosome and lysosomes
-Plant cells have a cell wall, chloroplasts, plastids, vacuole

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17
Q

Eukaryotes vs prokaryots

A

-Eukaryotes internal components organelles. cytoskeleton. Mitosis cell division
-Prokaryotes have no membrane. Nucleoid dna. Sexual and asexual

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18
Q

4 ROLES OF MEMBRANE PROTEINS *

A

Transport,
enzyme,
triggering signals,
Attachment and recognition

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19
Q

DEFINITION OF
Transport
Enzyme activity
Triggering signals
Attachment and recognition

A

Transport ions across membrane
Enzyme activity membrane proteins act as enzymes
Triggering signals binding chemicals trigger changes
Attachment and recognition internal and external membrane attachment point for cytoskeleton

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20
Q

main role of protien membrane 1/4
Transport

A

Transport ions across membrane

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21
Q

main role of protien membrane 2/4
Enzyme activity

A

Enzyme activity membrane proteins act as enzymes

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22
Q

main role of protien membrane 3/4
Triggering signals

A

Triggering signals binding chemicals trigger changes

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23
Q

main role of protien membrane 4/4
Attachment and recognition

A

Attachment and recognition internal and external membrane attachment point for cytoskeleton

24
Q

Integral membrane

A

Integral membrane protein embedded in lipid bilayer

25
Peripheral membrane
Peripheral membrane protein on surface
26
Passive transport
-moves substances across membrane without energy -diffusion drives passive transport
27
Active transport
Active transport uses energy to move molecules from low to high concentration, uses pumps
28
concentration gradient
Influence movement of solute, moves down gradient, energy required, atp
29
Diffusion
Diffusion is movement from high concentration to low
30
Osmosis
Osmosis diffusion of water across membrane
31
Hypotonic
Hypotonic lower solute concentration, can burst
32
Hypertonic
Hypertonic higher solute concentration, can shrink
33
Isotonic
Isotonic same concentration as others
34
Channel proteins
Channel proteins hydrophilic pathway, water molecules pass through
35
Carrier proteins
Protein carries and transport across bilayer
36
Primary active
Primary active directly utilizes energy, lower to high concentration, Na k pump,
37
Secondary Active
Secondary Active relies on primary energy, symport same direction, antiport opposite direction
38
** Na K
Na K pump sodium ions out, potassium ions in, ATP changes shape of protien, resembles nerve
39
Role of ATP in transport
-Atp used to move substances against their gradients, -immediate energy source -Used with exocytosis and endocytosis
40
Endocytosis (enter)
Endocytosis goes into the cell, carries proteins and large molecules into cell (enter)
41
Exocytosis (exit)
Exocytosis protein and waste out of cell (exit)
42
Phagocytosis
Phagocytosis cell eating
43
Pinocytosis
Pinocytosis cell drinking, water taken in
44
Receptor-mediated endocytosis
-Molecules taken in bound by receptors - forms vesicle fuses with lysosome
45
Role and process of exocytosis
Vesicle formation (waste is trapped), vesicle transport (waste moving towards membrane), membrane fusion, release
46
Lock and key model
Must have same shape for bonding
47
Enzymes function by
-lowering activation - TWISTING AND BENDING
48
Inducer and inhibitor
Inducer: increase activity Inhibit: slow things down
49
Enzyme activator
promotes enzyme function in turned off enzymes
50
** Selectivity Permeable
-allows certain molecules to pass through, large molecules cant pass without surgars
51
Protiens can be amphipathic
polar and non polar
52
Things that effect diffusion
high to low concentration and pressure
53
Tonicity
size of cell will change turgor
54
Result of osmosis
hypertonic
55
Phospholipid tails are.. Phospholipid heads are..
hydrophobic tail hydrophilic heads
56
Simple disfusion
small nonpolar substances move across membrane