Enzyme Effects

Question 1

Effect of enzyme concentration on initial rate of reaction

Answer 1

• The initial rate of reaction is directionally proportional to enzyme concentration.
• This is because there are more active sites, greater chance of a collision between enzyme and substrate.
• More enzyme-substrate complexes formed in a given time.
• So reaction goes faster.

Question 2

What happens to initial rate of reaction at high enzyme concentration.

Answer 2

• The initial rate of reaction may stop increasing.

- This is because the substrate concentration becomes limiting.