Enzyme Kinetics Flashcards
(8 cards)
1
Q
Rate equation
A
rate = v = a[S]/(b+[S])
2
Q
The energy of the enzyme and product should be _ than that of the enzyme and substrate.
A
Lower
3
Q
Which step governs the rate of reaction?
A
The step with the greatest energy barrier ES -> EP
4
Q
What is Km?
A
The substrate concentration needed to reach half of the maximum rate
5
Q
What happens when [S] < KM
A
Very few binding sites are occupied observed rate increases linearly with substrate concentration.
6
Q
What happens when [S] > KM?
A
Binding sites are saturated observed rate is independent of substrate concentration in this case vP = vmax
7
Q
Lineweaver-Burke plot
A
Plot 1/v against 1/[S]
Gradient is KM/Vmax
Intercept is 1/Vmax
8
Q
Km can also be considered a measure of the _ between the substrate and the enzyme
A
binding affinity
