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Flashcards in enzymes Deck (11)
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1
Q

what do R groups do

A

puts strain on the substrate bonds; helps reaction along!

2
Q

extracellular eznymes

A

break down polymers/ polysacharides that are otherwise too large to enter the cell

3
Q

temperature coefficient

A

qa0-> measure of how much rate of reaction increase with 10 degree rise in temperature

4
Q

differences in optimum temperature enzymes between different organisms

A

organism in hot conditions have higher optimum temperatures

vice versa

5
Q

why is there a rapid decline in rate ?

A

there only needs to be a slight changei n active sit after 40 degree temperature

6
Q

percusor enzymes

A

inactive; can damage cells and only acitvatedu nder standard coditions

need to undergo shape change to active site via cofactor addition

percussor protein=- apoenzyme , cofactor activates apoenzyme to holoenzyme

7
Q

coenzymes enzsure that

A

active sites are correctly formed !

8
Q

Q10=

A

R2/R1

9
Q

green box ( practical) : investigation into effects of differetn factors on enzyme activity!

A

use catalse :

catalyses breakdown ofhydrogen peroxide to water and oxygen - vol of oxygen gas collected used as measure of rate.

  1. liver tissue put into h2o2 solution - vol of oxygen released every 5 seconds measured

2nd experiment liver boiled for 5 minutes- then placed into h2o2 solution

(boiled liver rekeased much elss oxygen that normal liver )

10
Q

green box: serial dilutions

A

done in factors of 10 to produce range of concentrations .

useful even if conc of initial solution= unknown as htey give relative concentrations

.used to investgate effect ofchanging conc on enzyme activity, determinign numberso f microorganisms in culture

eg + 1 ml water to 9ml stock, gives 10 ml dilute solution- hence 1/10 dilution. repeat to get more dilution

11
Q

how can serial dilution be used for liver experiments ?

A

liver or potato has lots of catalse- broken down to make solution- solution contains catalse released from cells. serial dilutions give diff concs. effect of diff concs on rate of reaction- investigated by adding equal vol of h2o2 to each solution