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enzymes Flashcards

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1
Q

enzyme in Bilirubin enzymatic reaction

A

Bilirubin oxidase

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2
Q

highest elevation of CK >5xULN

A

Duchenne’s muscular dystrophy

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3
Q

CK tissue sources

A

BHS
Brain, heart (cardiac ), Skeletal muscle

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4
Q

CK Isoenzyme in severe tissue damage

A

CK-Mi

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5
Q

major CK isoenzyme in healthy individuals

A

CK-MM

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6
Q

CK Forward determination

A

Tanzer-Gilvarg

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7
Q

CK Reverse determination

A

Oliver-Rosalki

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8
Q

CK: Ref value:
Male: 15-160U/L (37°C)
Female : 15-130 U/L (37°C)

A
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9
Q

Tissue sources Of LDH

A

Heart, RBCS, Renal cortex, Lungs, Liver, Skeletal muscle

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10
Q

Normal pattern LDH

A

LD2>LD1

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11
Q

LDH pattern assoc. w/ AMI, Renal Infarction Hemolysis

A

LD1>LD2

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12
Q

highest elevation of LDH >5xULN

A

megaloblastic/pernicious anemia

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13
Q

Cold Labile LDH isoenzyme

A

LD5 (most) and LD4

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14
Q

ck enzyme labile when stored

A

ck 1

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15
Q

Forward method LDH determination

A

Wacker

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16
Q

Reverse LDH determination

A

Wroblewski-Ladue

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17
Q

Reference value LD
100-225 U/L @ 37°C

A
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18
Q

ck starts to increase AMI
a 4-6 hrs
b. 6-8 hrs
C. 12-24hrs

A

4-6 hrs

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19
Q

AMI,ck peaks at
a.48-72hrs
b. 12-24 hrs
c.18-24hrs

A

12-24 hrs

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20
Q

AMI,ck normalize in

a. 10 days
b. 4-5 days
C. 2-3 days

A

2-3 days

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21
Q

AMI, AST starts to elevate

a. 4-6 hrs
b.6-8hrs
C. 12-24hrs

A

6-8 hrs

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22
Q

AMI, AST peaks at

a.48-72hrs
b. 18-24hrs
C. 12-24 hrs

A

18-24 hrs

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23
Q

AMI, AST normalize in

a. 10 days
b.2-3 days
C. 4-5 days

A

4-5 days

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24
Q

AMI, LD starts to increase

a. 6-8 hrs
b. 4-6 hrs
C.12-24 hrs

A

12-24 hrs

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25
AMI, LDH peaks at a . 18-24 hrs b. 12-24hrs C.48-74 hrs
48-74 hrs
26
AMI, LDH Normalize a 4-5 days b. 10 days C.2-3 days
10 days
27
AST/SGOT tissue sources
Liver, heart, skeleletal muscle
28
ALT/SGPT tissue sources
Liver
29
ALT/SGOT >5XULN
Acute hepatocellular disorders
30
De Ritis Ratio
AST/ALT <1
31
no isoenzyme
ALT/SGPT
32
AST/ALT Determination
Reitman-Frankel w/ COD enzymatic reaction
33
AST: 5-35 U/L ALT: 7-45U/L
34
ALP tissue sources
liver, bone, placenta, intestine (Spleen, kidney)
35
Highest ALP elevation >5ULN
paget's disease/osteitis deformans
36
Electrophoresis ALP is oenzyme anodic - cathodic
Liver Bone PIacenta Intestine
37
Heat stability in ALP isoenzymes most heat stable -- heat labile
placenta, intestine, liver, bone
38
urea inhibits
Bone
39
phenylalanine inhibits
placental, intestinal, carcinoplacental isoenzyme
40
Levamisole inhibits
Liver and bone
41
Leucine inhibits
Nagao
42
Intestinal depends on the blood group and secretor status more likere to be found in
B and O secretor individuals
43
ALP ref. method
Bowers-McComb
44
ALP Ref value 30-90 U/L
45
Acute pancreatitis, AMS occur : 2-12hrs peak: 24hrs normalize 3-4 days
46
Acute pancreatitis, Lipase occur : 4-8 hrs peak: 24 hrs Normalize : 8-14 days
47
Ref. AMS determination
Saccharogenic Method
48
ams determination S accharogenic - I Inc. keducing sugar (Alcohol) A myloclastic - decrease color intensity C homogenic- Increas color intensity C OD Enzymatic mtd-Increase ABSORBANCE 340 nm
49
AMS NV Serum: 25-130 U/L Urine: 1-15 U/h (faster clearance due to small size)
50
Salivary AMS inhibited by
Wheat germ Lectin
51
Lipase determination
Cherry Crandall Method
52
Cherry Crandall Method uses: Olive Oil or Triolein as substrate Reagent: TAG measured: Titration
53
lipase Ref. value 0-1.0 U/mL (<38 U/L)
54
GGT increase
Alcoholic liver disease
55
GGT REF.value male: 55 U/L Female : 38 U/L
56
Increase 5 'Nucleotidase
Intrahepatic cholastasis
57
Increase ALP, GGT, 5'nucleotidase
LIVER
58
Increase ALP N° GGT, 5'nucleotidase
BONE
59
ACP tissue sources
HIGH: prostate glands Low: Bone, placenta, erythrocyte, platelets
60
ALP determination
Roy method
61
Roy method uses
Thymolphthalein monophosphate
62
Prostatic ACP is inhibited by
tartrate
63
RBC ACP is ihibited by
Formaldehyde and copper
64
Ref range ACP total ACP: male: 2.5-11.7U/L Female : 0.3-9.2 U/L prostatic ACP male: 0.2-5.0 U/L Female : 0.0-0.8U/L
65
G6PD tissue sources
RBCs, adrenal glances, thymus, lymph nodes, spleen
66
Drug Induced Gemolitic anemia def. in what enzyme
G6PD
67
Antimalarial drugs/primaquine can cause des in what enzyme
G6PD - drug induced hemolytic anemia

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