Enzymes Flashcards

(37 cards)

1
Q

How does a ph that is very different to the optimum affect enzyme activity?

A

The change in ph will distort the enzymes active site denaturing it so that it can no longer fit the specific substrate

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2
Q

Describe the effect of the ph on the rate of an enzyme controlled reaction

A
  • the rate of enzyme catalysed reaction is the fastest at the optimum ph
  • if the ph is too high or low, the enzyme will work less efficiently and the active site may be denatured
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3
Q

If the temperature increases above the optimum, how does this affect enzyme function?

A

The active site will be distorted as the enzyme denatures and so it will no longer fit the substrate

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4
Q

Why doe the rate of an enzyme controlled reaction increase when the temperature increases?

A
  • as the temperature increases the particles have more kineti energy
  • this increases the chance of collisions between molecules being successful and leading to a reaction
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5
Q

Describe the effect of temperature on the rate of an enzyme controlled reaction

A
  • as the temperature increases, so doe he rate of reaction
  • once the temperature exceeds the optimum, the enzyme denatures and the rate of reaction decreases
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6
Q

What are the fur factors that affect the rate of enzyme controlled reactions

A
  • ph
  • temperature
  • substrate concentration
  • enzyme concentration
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7
Q

What does the statement enzymes are highly specific mean?

A

The active site of the enzyme must bind to a substrate that has a complementary shape and so only specific reactions can be catalysed.

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8
Q

Describe enzyme action

A
  • a substrate that fits the specific active site of the enzyme binds to create and enzyme-substrate complex, a reaction occurs catalysed by the enzyme and the products are released
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9
Q

Why are enzymes necessary in biological systems?

A

They speed up reactions so that less energy is needed for the reaction to occur

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10
Q

Describe the structure of enzymes

A
  • enzymes are proteins that contain a highly specific active site that fits a specific substrate
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11
Q

What are enzymes?

A

Biological catalysts that speed up the rate of reactions

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12
Q

What is a catalyst?

A

A substrate which increases the rate of reaction without being used up

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13
Q

Pepsin

A

Breaks proteins into polypeptides.
Protein digesting enzymes produced by the pancreas

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14
Q

Three types of digestive enzymes

A
  • Carbohydrates
  • proteases
  • lipeases
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15
Q

Example of a carbohydrate enzyme

A

Amylase

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16
Q

Where is amylase made?

A
  • Salivary glands
  • pancreas
  • small intestine
17
Q

What are carbohydrates such as starch broken down into?

A

Simple sugars such as glucose

18
Q

Where are proteases made?

A
  • stomach
  • pancreas
  • small intestine
19
Q

What are the products of protein digestion?

20
Q

Where do proteases work?

A

Stomach and small intestine

21
Q

What are the products of lipid digestion?

A

Glycerol and fatty acids

22
Q

Where are lipeases made/

A

Pancreas and small intestine

23
Q

Where is bile made?

24
Q

Where is bile stored?

A

The gall bladder

25
Two functions of bile
- neutralises hydrochloric acid from the stomach - emulsifies fat
26
What does the term emulsifying fats mean?
- break fats into tiny droplets
27
Why does emulsification of fats make digestion faster?
Gives a bigger surface area for lipeases enzymes to work on
28
Why is it important that bile is an alkaline?
To neutralise stomach acid and create optimum conditions for enzymes in small intestine
29
What is the test for starch? And colour change
- Iodine solution - brown/orange - blue/black
30
Test for presence of proteins and colour change?
- biuret - blue - purple
31
Test for lipids and colour change
- Sudan III / ethanol - Sudan III - mixture will separate into two layers top one is red - ethanol - cloudy white emulsion
32
Test for presence of reducing sugars and colour change
- Benedict’s test - blue/green - yellow - brick red with increasing amounts of sugar
33
What is the name of the protease that is found in the stomach called?
- pepsin
34
Where does the gall bladder release bile into?
- small intestine
35
Where does the absorption of products of digestion happen?
- small intestine
36
Function of hydrochloric acid
- kills bacteria - makes acidic conditions for pepsin - works best at ph 2
37
Function of digestion
- breaks big molecules into smaller ones tat can easily be absorbed