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Biochemistry > Enzymes > Flashcards

Enzymes Flashcards

1
Q

Theories of mechanism of activation?

A

1) Lowering of activation energy
2) Fischer’s template theory
3) Michaelis menten theory
4) koshland’s induced fit theory

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2
Q

Class 1 of enzymes?

A

Oxidoreductase

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3
Q

Class 2 of enzymes

A

Transferases

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4
Q

Class 3 of enzymes

A

Hydrolase

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5
Q

Class 4 of enzymes

A

Lyase

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6
Q

Class 5 of enzymes

A

Isomerases

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7
Q

Class 6 of enzymes

A

Ligases

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8
Q

Factors influencing enzyme activity?

A

1)Velocity proportional to enzyme Concentration
2) temperature:bell shaped curve
Increasing :with increase rate of reaction
Decreasing :dt denaturation of protiens
3) substrate concentration/saturation concentration.
Hyperbolic curve
4) Michaelis-menten equation
Velocity of reaction varies with substrate concentration
Vi=(vmax * s) / km + s
5)lineweaver-burk plot
Double reciprocal plot
Y= a x + b

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9
Q

Stating (CI)inhibits? And clinical use

A
  • Hmg coA

- lowering cholesterol

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10
Q

Dicumora(CI)l inhibits and use?

A

Vit k epoxide reductase

Anti coagulant

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11
Q

Allopurinol (CI)inhibits and use

A

Xanthine oxidase

Gout

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12
Q

Penicillin (CI)inhibits and use

A

Transpeptidase

Antibiotic

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13
Q

Sulfonamide (CI) inhibits and use?

A

Pteroid synthetase

Antibiotic

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14
Q

Trimethoprim(CI) inhibits and use?

A

Dihydrofolate

Antibiotic

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15
Q

Primethamine (CI)inhibits and use

A

FH2 reductase

Malaria

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16
Q

Methotrexate (CI)inhibits and use

A

FH2 reductase

Cancer

17
Q

6-mercapto purine (CI)inhibits and use?

A

Adenyl succinate synthetase

Cancer

18
Q

5-FU (CI)inhibits and use?

A

Thymidylate synthetase

Cancer

19
Q

Azaserine (CI) inhibits and use

A

Phosphorybosyl amifotransferase

Cancer

20
Q

Cytosine arabinoside (CI)inhibits and use?

A

DNA POLYMERASE

cancer

21
Q

Acyclovir (CI)inhibits and use

A

Dna POLYMERASE

Anti viral

22
Q

Neo stigmine (CI)inhibits and use

A

Ach -esterase

Myasthenia

23
Q

Alpha methyl dopa(CI) inhibits and use

A

Dopa decarboxylase

Htn

24
Q

Oseltamavir (tamiflu)(CI) inhibits and use

A

Neuraminidase

Influenza

25
Q

Digoxin (CI)inhibits and it’s use?

A

Na+ k+ ATPase

Heart failure

26
Q

Cyanide (nci) inhibits?

A

Cytochrome oxidase

27
Q

Iodoacetate (NCI) inhibits

A

Glyceraldehyde 4 phosphate

28
Q

Fluoride (NCI) inhibits

A

Enolase

29
Q

BAL(NCI) inhibits

A

SH group of many enzymes

30
Q

Fluoroacetate (NCI)inhibits

A

Aconitase

31
Q

Di-isopropyl fluorophosphate(NCI) INHIBITS

A

Serine proteases

32
Q

Arsenite (NCI) Inhibits

A

Alpha ketoglutarate dehydrogenase

33
Q

Disulfiram (antabuse)(NCI) INHIBITS

A

Aldehyde dehydrogenase

Biochemistry (2 decks)

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