Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

CC 2 LEC > Enzymology > Flashcards

Enzymology Flashcards

(39 cards)

Start Studying
1
Q

Proteins produced by living cells that hasten chemical reactions in organic matter.

A

Enzymes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Measured in terms of their activity and not in terms of their absolute value

A

Enzymes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

T or F: The higher the enzyme concentration, the faster the reaction.

A

True (more enzyme is present to bind the substrate)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

10 Factors affecting enzyme reaction.

A
  1. Enzyme concentration
  2. Substrate concentration
  3. Cofactors
  4. Inhibitors
  5. Isoenzymes
  6. Temperature
  7. Hydrogen ion concentration or pH
  8. Storage
  9. Hemolysis
  10. Lactescense or milky specimen
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Example of activators. (CCMPZ)

A
  • calcium
  • chloride
  • magnesium
  • potassium
  • zinc
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Examples of metalloenzymes. (CC)

A
  • catalase
  • cytochrome oxidase
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

3 types of inhibitor.

A
  • competitive inhibitor
  • non-competitive inhibitor
  • uncompetitive inhibitor
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Physically binds to the active site of an enzyme

A

Competitive inhibitor

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Does not compete with the substrate but look for areas other than the active site.

A

Non-competitive inhibitor

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Binds to the enzyme-substrate complex

A

Uncompetitive inhibitor

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Enzymes are active on what temperature?

A

25C, 30C, 32C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Optimum temp. for enzymatic activity

A

37C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Rate of denaturation is usually significant at what temp.?

A

40 - 50C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Temp. that may result in activation of enzymes

A

60-65C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Most physiologic enzyme reaction occur in what pH?

A

range 7-8 pH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Ideal temp. for preservation of enzyme

17
Q

Ideal storage temp. for substrate and coenzymes

Study These Flashcards
A

2 - 8 degree C

18
Q

Ideal temp. storage for LDH (LD4 and LD5)

Study These Flashcards
A

22Cto room temp.

19
Q

Hemolysis decreases enzyme concentration. Lactescense/milky spx. increases enzyme concentration.

Study These Flashcards
A

Both statement is false.
Hemolysis - increases
Lactescence - decreases

20
Q

Catalyze the removal or addition of electrons (redox reaction)

Study These Flashcards
A

Oxidoreductases
- CO, LDH, MDH, ICD, G-6-PD

21
Q

Catalyze the transfer of a chemical group other than hydrogen from one substrate to another.

Study These Flashcards
A

Transferases
- CK, AST, ALT, OCT

22
Q

Catalyzehydrolysis or splitting of a bond by the addition of water (hydrolytic reactions)

Study These Flashcards
A

Hydrolases
- Esterases: ACP, ALP, CHS, LPS
- Peptidases: Trypsin, Pepsin, LAP
- Glycosidase: AMS, Galactosidases

23
Q

Catalyze removal of groups from substrates without hydrolysis. The product contains double bonds.

Study These Flashcards
A

Lyases
- Glutamate decarboxylase, Pyruvate decarboxylase, Tryptophan decarboxylase and Aldolase

24
Q

Catalyze the intramolecular arrangement of the substrate compound

Study These Flashcards
A

Isomerases
- Glucose phosphate isomerase and Ribose phosphate isomerase

25
Catalyze the joining of two substrate molecules, coupled with breaking of the pyrophosphate bonding ATP or similar compound.
Ligases - Synthase
26
Based on the premise that the shape of the key (substrate) must fit into the lock (enzyme)
Emil Fisher's/Lock and Key theory
27
Based on the substrate binding to the active site of the enzyme
Kochland's/Induced fit theory
28
An enzymes combines with only one substrate and catalyzes only one reaction is known as ________
Absolute specificity
29
Enzymes combined with all the substrates in a chemical group is called _______
Group specificity
30
Enzymes reacting with specific chemical bonds is known as _________
bond specificity
31
1 micromole of substrate/minute
International Unit (IU or U)
32
1 mole of substrate/second
Katal unit (KU)
33
Enzymes are measured in terms of:
1. Change in substrate concentration 2. Change in product concentration 3. Change in coenzyme concentration
34
The reaction rate depends only on enzyme concentration.
Zero-order reaction
35
the reaction rate is directly proportional to substrate concentration
the reaction rate is directly proportional to substrate concentration
36
It is a nonspecific enzyme capable of reacting with many different substrates.
Alkaline phosphate
37
functions to liberate inorganic phosphate from an organic phosphate ester with the concomitant production of an alcohol.
Alkaline phosphate
38
Major tissue sources of Alkaline phosphate (LBPI)
Liver, Bone, Placenta, Intestinal
39
Reference value of Alkaline Phosphate
30-90 U/L

CC 2 LEC (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions