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Flashcards in Escherichia coli Deck (34):
1

Specifics

Lactose positive
oxidase negative
motile rods
O antigen 26, K capsule 60 (B6), F fimbrae (41) and H flagella (11)

2

E. coli in pigs

ETEC
VTEC
EPEC
UTI
MMA
Septicemia

3

ETEC Virulence factors neonatal

F5, F6, F41
non hemolytic
*everything passed neonatal is hemolytic

4

ETEC Virulence <4 weeks

F4, LT, EAST1

5

ETEC Virulence factors Weaning diarrhea

F4, F18, LT, EAST1

6

F4 Adhesion factor

pigs only, on plasmids,

7

F5 Adhesion factor

pigs and bovine, only receptor for very young animals (neonatal), T dependent expression

8

F6 Adhesion factor

extra chromosonal, neonatal

9

F41 Adhesion factor

pigs and bovine, chromosomal

10

F18 Adhesion factor

Edema disease

11

ETEC Heat labile toxin

high molecular weight, strong Ag and used to make vaccines

12

ETEC Heat stable toxin

low molecular weight, little antigenic

13

ETEC Clinical signs

watery diarrhea, very smell feces, dehydration

14

ETEC pathogenesis

ascend from lower bowel or oral uptake and then colonizes small intestine

15

ETEC epidemiology

infection get from intestine of healthy sows
equilibrium between maternal immunity and infection pressure
low hygiene

16

ETEC Tx and Prev

hydration
antimicrobials
vaccinate sows

17

VTEC in pigs age affect

<3 weeks after weaning

18

VTEC Clinical signs pig

Edema disease
swollen eye lids
hoarse voice
diarrhea with mixed and ETEC and VTEC

19

VTEC virulence factor in pig

F18
Exotoxin- VT2e and STX2e media necrosis

20

VTEC Prev and treatment in pigs

Affected pigs: fast to eliminate and then antimicrobial
Non affected pigs: antimicrobials and increase in 2-3 days
Prevent add zinc oxide

21

EPEC (enteropathogenic) in pigs

rare
weaning pigs - diarrhea
virulence factor T3SS (needle and injection)
Diagnostics- Isolation and PCR (Eae- intimin gene)

22

UTI in pigs

CS: anorexia, vaginal exudate
Tx/Prev: culling due to fertility problems, antibitotics for 3 weeks

23

MMA in pigs

48 hours after farrowing
post partum dysgalactiae syndrome- no milk production, colostrum and septicemia
Edx: hereditary, hormonal, feed, infectious agents

24

Septicemia in pigs

secondary to enteric infection
rarely primary

25

ETEC in cows

age affected less than 3 days
Dx: ELISA, culture and virulence factors (agglutination/PCR)
Virulence factors: F5, F41, CS31A, STa, Stb
Therapy: separate sick animals, hydration, antibiotics extremely high resistance, vaccinate dams, colostrum

26

EPEC (enteropathogenic)
EHEC (enterohemorrhagic)- Zoonotic
in cow

Age 1-8 weeks
CSS: mucoid diarrhea with/without blood
mainly large intestine
Therapy: antibiotics
Virulence factors:
EPEC- initimin eae, EHEC intimin eae + VT1 and VT2

27

Mastitis in cow

CS: udder swelling, redness, lesions
prev/tx: hygiene and systemic Antibiotics
no known virulence factors

28

Septicemia in cow

CS: hyperacute- acute mortality and shock,
acute- general symptoms, fast mortality,
slower- organ localization: polyarthiritis, pneumonia, pleuritis, meningo- encephalitis
Pathogenesis:
PO--> lymph tissue--> septicemia / umbilical cord-->septicemia

29

E. coli in dogs and cats (ETEC, EPEC, VTEC, EIEC)

Frequent with other pathogens
Virulence factor CNF1- Ecoli in puppies

30

Cysitis/ pyometra

Cystitis inflammation in bladder, pyometra uterine infection
cats seldom get cystitis, high osmolarity of urine
Virulence factors: F12, F13 (associated with human UTI)
Specific (o2,o4,o6,o83)

31

E. coli in birds

Clinical signs and Pathogensis

most important bacterial disease in broilers and layers
Neonatal colibacillosus --> contamination of egg, neonatal or manipulation of chick that leads to yolk rest infection called omphalitis--> sepsis/death/retardation
Respiratory colibacillosus--> excretion leads to increase of infection pressure--> inhaled--> cause infection or can damage resp epithelium
Peritonitis--> decrease egg production

32

Ecoli in birds- prevention

hygiene, decrease stress, control temp, vaccines, difficult to treat

33

Ecoli is broilers

Serotype O78, O2
CS: scabby hips/necrotic dermatitis in broilers
Use feathers for diagnosis, usually at slaughter
Prev: low density of animals

34

E coli in rabbits
RPEC/EPEC

virulence factor intimin eae
Dx- isolate and identify serotype
Tx- antibiotics