Estrous Cycle Flashcards

(54 cards)

1
Q

Estrus (noun)

A

Period of sexual excitability when a female will accept the male for breeding

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2
Q

Estrous (Adjective)

A

relating to or a characteristic of estrus

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3
Q

Estrous Cycle

Continually polyestrous

A

Regular estrous cycles throughout the year

NOT responsive to photperiod

Bovine, Porcine, Some Ovine breeds

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4
Q

Estrous Cycle

Seasonal Polyestrous

A

Regular estrous cycles during part of the year

Responsive to photoperiod

Short Day Breeders: ovine, caprine, cervids

Long-day Breeders: Equine, Feline, Hamsters

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5
Q

Estrous Cycle

Diestrous

A

Two estrus per year

Domestic Canine

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6
Q

Estrous Cycle

Seasonally Monoestrous

A

One cycle per year

African breed dogs, bears, wolves, foxes

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7
Q

Bovine Estrous Cycle Periods

A

Estrus

Metestrus

Diestrus

Proestrus

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8
Q
A

Bovine Estrous Cycle

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9
Q

Bovine

Estrus

A

Standing ot be mounted

Lasts 20 hours

Progesterone levels are low

Estrogen levels are falling

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10
Q

Bovine

Metestrus

A

No longer “Standing”

Ovulation 10-15 hrs after estrus ends

Lasts 3-5 days

CL is being developed

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11
Q

Follicle Growth

1 day after onset of estrus

A

Concentrations fo FSH decrease after initial surge

Growth of numerous follicles contine

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12
Q

Bovine

Metestrus

Follicle growth Day 2 post estrus

A

Progesterone concentrations begin to increase

FSH concentrations remain above basal levels

Dominance of growing follicles begin to affect smallest follicles (inhibin)

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13
Q

Bovine Follicle Growth

Day 3 post estrus

A

Dominant and subordinate follicles secreting estradiol and inhibin

These two compounds are suppressing the growth of other follicles

If the largest follicle was ablated or aspirated, the largest subordinate would continue to grwo and become dominant

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14
Q

Bovine Follicle Growth

Day 4 post estrus

A

CL size and progesterone concentrations increase substantially

Divergence of largest follicle form subordinates between day 3-4

Subordiante follicles become atretic

Dominant follicle acquires LH receptors

GnRH (exogenous - injected by human) induced LH surge can ovulate the domiant follicle

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15
Q

Bovine Follicle Growth

Day 5 post estrus

A

Few Corpora Lutea responsive to exogenous PGF2a

GnRH induced LH surge can ovulate the dominant follicle

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16
Q

Diestrus

A

Period of the CL

Lasts about 12 days

High levels of Progesterone

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17
Q

Bovine Follicle Growth

Days 6-14 post estrus

A

Neally all Corpora Lutea are responsive to exogenous PGF2a

GnRH induced LH surge can ovulate the dominant follicle if follicle is not too mature

FSH suppressed

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18
Q

Bovine Follicle Growth

Days 15-17

A

Nearlly all Corpora Lutea are responsive to exogenous PGF2a

GnRH induced LH surge will ovulate the new dominant follicle

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19
Q

Bovine Proestus

A

CL is regressing

Lasts 2-3 days

Progesterone levels decreaseing

Estrogen levels Increasing

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20
Q

Bovine Follicle Growth

Day 18

A

CL regression induced endogneously

GnRH induced LH surge will ovulate the new dominant follicle

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21
Q

Bovine Follicle Growth

Day 19 & 20

A

CL regression induced endogneously

GnRH induced LH surge will ovlulate the new dominant follicle

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22
Q

Equine Cycle is divided into two parts

A

Estrus

Diestrus

*Anestrus

23
Q

Anestrus

A

Complete absence of estrous cycles during short days

24
Q

Equine

Estrus

A

Aka “Heat” or “in heat”

Aka Follicular phase of the estrous cycle

Lasts 7 days

THe mare is receptive to the stallions advances

Progesterone levels in blood are low

Estrogen levels in blood are high

LH surge leasts longer in the mare than other species

Estrus Ends abotu 24-48 hours after ovulation

25
Equine Estrus Vaginal Speculum Examinaition
During Estrus, progesterone levels are low and the cervix is swollen with relaxed folds which hang down over hte open orifice
26
Equine Estrus Teasing
Obvious interest in the stallion, urination, vulva eversion, and squatting
27
Equine Diestrus
Aka Luteal Phase of the Estrous Cycle Time of progesterone dominance Lasts 14-15 days The mare refuses the stallion Progesterone comes from the CL Endogenous PGF2a is secreted by uterus if no maternal recognition of pregnancy by day 13-14 PGF2a causes CL lysis and a rapid decline in progesterone production the last few days of diestrus
28
Equine Diestrus Vaginal Speculum Examination
During diestrus, progesterone levels are high and the os cervix is tightly closed and surrounded by firm, well-defined folds
29
Equine Diestrus Teasing
Obvious hostility to the stallion, ears back, striking, biting, squealing
30
Canine cycle is divided into 4 parts
Anestrus Proestus Estrus Diestrus Interestrus = diestrus + anestrus
31
Canine Vaginal Cyctology
Evaluating vaginal cytology is an important clinical skill in canine repro management Use cotton-tipped swab to gently exfoliate cells form the vaginal vault Decrease estrogen means the bitch is not interested in breeding and the vaginal lining is thin. Vaginal cells are close to the blood supply and very healthy looking Increased estrogen means the bithc is interested in mating and the vagina needs to “toughen up” and become thicker. The vaginal cells on the surface are now far from the blood supply and are dead
32
Canine Anestrus
Lasts for a variable amount of time after diestrus ~ 90-150 days Anestrus is a time of mandatory endometrial repair Neither male nor female shows sexual interest the vulva appears normal, not swollen or edematous Progesterone is at baseline concentrations \<1ng/ml Prolactin secretion by the pituitary may promote anestrus, becuase prolactin inhibitors can be used to terminate anestrus
33
Canine Anestrus Vaginal Cytology
Relatively constant One sees primarily proabasal and small intermediate epithelial vaginal cells Neutrophils may or may not be present and RBCs are usually absent Bacteria may or may not be seen
34
Canine Proestrus
Averages 5-10 days Males are interested in the female, but female is not interested in males The vulva is swollen because of estrogen secretion by the growing follicles THere is serosanguinous discharge form the vulva that results form diapedesis of RBCs through urterine vessels The most reliable clinical sign of hte onset of proestrus is noting the first day of a bloody vaginal discharge LH has an increased pulse-frequency FSH suppressed Estrogen comes from the growing follicles and peaks at end of proestrus Testosterone is relatively high at the end of proestrus. This may result from a “backup” in estrogen synthesis. This may result in some masculine behavior by the bitch such as mounting other dogs
35
Canine Early Proestrus
Similar to bithc in anestrus except, for the presence of varying ninbers of red blood cells RBCs numerous parabasal cella nd intermediate cells Neutrophils are common although usually not abundant and bacterai the background is granual or “dirty”
36
Canine Mid-proestrus
THe first evidence of continued estrogen effect on the vaginal cytology is the disappearance of neutrophils Fewer parabasal and small intermediate cells, more large intermediate and superficial intermediate cells RBC may ro may not be present The background is dirty or clear
37
Canine Late proestrus
No neutrophils, RBC are variable background is clear Greather than 80% of the vagianl cells are superficial cells, with pyknotic nuclei or anuclear superficial celsl
38
Canine Estrus
Average 9 days THe make and female are both interested in each other The estrous behavior results from the abrupt decline of estrogen following the peak in leat proestrus
39
Canine Diestrus
Time of progesterone dominance the hormonal events of diestrus are essentially the same in both pregnant and non-pregnant bitches The female refuses male advances Progesterone comes form the CL and rises through the first half of diestrus Endongenous PGF does not appear to be luteolytic in the bitch Exogenous prostaglandins are luteolytic in the bitch as early as 5 days after the beginning of diestrus
40
Canine Diestrus Vaginal cytology
clearly demarcated from that from a bitch that is nearing the end of estrus within 24-48 hours at the end of estrus the percentage of superficial cells falls to approxiamately 20%, with the remainder of cells usually being intermediate cells neutrophils occasionally reappear ovulation happend 6 days ago
41
Feline Proestrus
averages 1 day or less rejects males, but displays rolling, head rubbing, vocalization, crouching
42
feline estrus
averages 6 to 7 days accepts male - deviation of tail to one side and allows male to grasp dorsum of neck Intestsification of proestrus behavior: rolling, head rubbing, vocalizing, crouching INDUCED OVULATOR
43
Induced ovulator
if mating does not occur, LH is not released Multiple matings results in higher and more prolonged LH release Ovulation occurs about 50 hours after LH peak
44
Feline Metestrus
lasts about 1-2 weeks if not mated and LH is not released - no ovulation occurs and follicles regress during metestrus If queen does not ovulate - progesterone does not rise above base-line
45
Stage of Estrous Cycle Determination Bovine
One time palpation of ovarian structures will NOT determine chates of cycle Uterine tone differs based on dominate steroid homrone: relaxed with progesterone, turgid with estrogen; not reliable Progesterone concentration in milk Estrus detection - standing to be mounted
46
Stages of estrous cycle determination bovine Estrus detection
breed AI 12 hours after first detection in estrus secondary signs: riding other cows, mud on back, increased activity Gomer (penile deviation) bull, androgenized cows Color-change patches or tail-head paint ot indicate riding Electronic mounting detector Electronic behavior monitoring
47
Stage of Estrous Cycle determination Ovine
Estrus detection: Ewes are subtle - difficult to detect in estrus To detect estrus in order to utilize AI - need a vasectomized ram
48
Stage of estrous Cycle determination Caprine
Estrus Detection Does will vocalize more and increased tail wagging Vulva becomes reddened and vaginal discharge may be noted To detect estrus in order to utilize AI - need a vasectomized buck
49
Stages of Estrous Cycle Determination Equine
One-time palpation of ovarian structures will NOT determine stages of cycle Serial ovarian palpation of folicle size can be used to determine timing of ovulation Cervical tone differs based on dominate steroid hormone Estrus detection - teasing witha stallion
50
Stages of Estrous Cycle Determination Equine Estrus Detection
daily during breeding season take at least 15-20 minutes Estrus behavior may be suppressed in: maiden mares, timid mares, mares with foals, during inclement weather, or early in the breeding season
51
Stage of Estrous Cycle Determination Porcine
Standing to be mounted Twice daily is recommended Boars and females brought to neutral pen or boar is walked next to femal pen Boar-odor sprays or audio recordings of boar “chant” can be used in absence of a live boar Handler applies pressure to females back in the presence of a boar - females in heat will stand firmly
52
Stage of Estrous Cycle Determination Canine
Estrus detection - most common method for natural mating Vaginal Cytolog - NOT precise enough to use alone for AI Serum Progesterone
53
Stage of Estrous Cycle determination canine serum progesterone levels
First rise in P4 \>1ng/ml is correleated with LH peak Ovulation begins 24-48 hours later, and oocytes are fertile 24-48 hours after ovulation Measure every 2 days starting on day 5 of proestrus Breed 4-6 days after first rise in P4 Breed first day P4\>5-7.5ng/ml Breed 72hrs after first day P4 \>5-7.5ng/ml Frozen semen)
54
Stage of Estrous cycle determination Canine Serum LH
used in conjunction with P4 measurments Typically measured every day starting on Day 5 of proestrus There are several rises in LH prior to the ovulatory peak This assay is not always used