EVERYTHING EVER Flashcards

I NEED TO PASS (577 cards)

1
Q

_________ is the scientific study of organisms, living and fossil.

A

Biology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q
  • Made up of a common set of chemical compounds
  • Made up of cells
  • Use molecules in the environment to make new molecules
  • Extract energy from the environment and use it to do work
    -Regulate internal environment (Homeostasis)
  • Contain genetic information that enables them to develop, function, and reproduce
  • Use a universal genetic code to build proteins
  • Exists in populations that evolve over time
A

The common characteristics of all life forms

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

All life’s similarities in chemical composition, cell structure, and genetic code are due to what?

A

Common Ancestry

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Earth formed ____________ - ______________ ago, but it was _______________ or more before life evolved.

A

4.6 billion and 4.5 billion years; 600 million years

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q
  • Nucleic Acids that reproduce themselves and serve as a template for protein synthesis
  • Enclosure of Biological molecules by memebranes made of fatty acids similar to liposomes
A

What are the elements that are essential to life?

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

A spherical structure contained by a membrane of phospholipids.

A

Liposomes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What are single-celled organisms called?

A

Prokaryotes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What are the two main groups of Prokaryotes?

A

Bacteria and Archaea

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What major category of life has membrane-enclosed organelles and nuclei?

A

Eukarya

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is the sum total of chemical reactions that occur in an organism, or some subset of a total (as in respiratory metabolism)

A

Metabolism

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What contains genetic information?

A

Nucleus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is the metabolic process carried out by green plants and some microorganisms by which visible light is trapped and energy is used to synthesize compounds such as ATP and glucose? This changed the nature of life on Earth life and the planet.

A

Photosynthesis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What are early photosynthetic cells that are similar to present-day prokaryotes?

A

Cyanobacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

This type of metabolism occurs in the presence of oxygen?

A

Aerobic Metabolism

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

This type of metabolism occurs in the absence of oxygen?

A

Anaerobic Metabolism

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Which is more efficient Aerobic Metabolism or Anaerobic Metabolism?

A

Aerobic Metabolism (most organisms use this type of metabolism)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Accumulating O2 lead to what?

A

The Ozone layer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What does the ozone layer absorb?

A

Damaging UV Radiation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

The division of labor is such that different cell types become responsible for different functions. This allowed larger and more efficient.

A

Cell Specialization

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

A group of similar cells is organized into a function unit.

A

Tissue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Different types of tissue come together to form ________ to serve a specific function; this is a body part

A

Organs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Organs can be grouped into ________________

A

Organ Systems

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

What is the hierarchy of biological organisms?

A

Atoms, small molecules, large molecules, cells, organism, population

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Movement; biochemical ____; electrical ____ in the nervous system

A

Work

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Requires nutrients from the enviroment
Organism
24
Energy can be stored ___
Fat
25
Organisms regulate their internal environment; Maintenance of the narrow range of conditions in the internal environment
Homeostatis
26
Regulation that requires information about the internal and external to be converted into neural signals
Sensory Mechanism
27
Adaptations that allow the organism to alter its physiological and behavioral state | Effect
Effector Mechanism
28
Integrate information and enable communication between sensors and effectors; transfer information between cells, organs, and organisms.
Signaling Mechanism
29
Hierarchy of the biological world
Population, Community, Ecosystem, and Biosphere
30
A major ecosystem that covers a broad geographic area with distinctive physical features and communities are known as _________
Biomes
31
All biomes make up the _______
Biosphere
32
Who showed that inherited traits exist in genes?
Gregor Mendel
33
_____ are the discrete units of inhertience
Genes
34
Sum total of all the DNA molecules in an organism; The complete DNA sequence for a particular organism and individual.
Genomes
35
DNA consists of long sequences called _____________; The basic chemical unit consists of a pentose sugar, a phosphate group, and a nitrogen-containing base
Nucleotides
36
The Discovery of _____ in the mid-1900s transformed biological science; The fundamental hereditary material of all living organisms.
DNA (Deoxyribonueclic Acid)
37
Change in the nucleotide sequence, are harmful, but lead to new adaptations.
Mutations
38
Group of individuals of the same species that interbreed
Population
39
Results in a change in the genetic makeup of populations over time; is the major unifying principle of biology.
Evolution
40
Compiled factual evidence for evolution and proposed that all organisms are descended from a common ancestor. Argued that differential survival and reproduction could acocunt for much of the evolution of life.
Charles Darwin
41
The differential contribution of offspring to the next generation by various genetic types belonging to the same population. Leads to adaptations: structural, physiological, or behavioral traits that enhance an organism's chances of survival and reproduction.
Natural Selection
42
Humans selectively breeding of plants and animals to obtain desired traits
Artificial Breeding
43
The scientific study of entire sets of genes and their interactions; can be used to compare organisms, leading to the field of ________
Genomics
44
The field of _____________ manages and analyzes sequencing data
Bioinformatics
45
A graphic representation of the lines of descent among organisms or their genes
Phylogenetic Tree
46
A taxonomic naming system in which each species is given a ________, a genus name followed by a species name.
Binomial (Homo sapiens)
47
A group of related, similar species recognized by taxonomists with a distinct name used in binomial nomenclature
Genus (Homo)
48
Quantified observations about a system under study
Data
49
1. Observation 2. Ask Question 3. Form Hypotheses 4. Make Predictions 5. Design and Conduct an experiment
Scientific Method
50
Uses observations or facts to develop a tentative answer or hypothesis
Inductive logic
51
Used to predict what facts would also have to be true to be compatible with the hypothesis
Deductive logic
52
A tentative answer to a question, from which testable predictions can be generated
Hypothesis
53
____________ manipulate one factor while holding other variables constant
Controlled experiment
54
____________ compare data gathered from populations that differ in multiple ways
Comparative Experiment
55
Include a small group of species that are the subject of extensive research. They are organisms that adapt well to laboratory situations, and findings from experiments on them can apply to a broad range of species (Zebrafish, Fruitflies)
Model Systems
56
A statistical test starts with a ________________ the premise that observed differences are the result of random variations that arise from drawing two finite samples from a population. | The claim that the effect being studied does not exist.
Null Hypothesis
57
- Science must be testable - Have the potential to be rejected - Depends on reproducible and quantifiable evidence
Characteristics that distinguish Science and Non-science
58
-Increasing knowledge of plant and animal biology - Recombinant gene technology - Biological Research led to higher yield crop varieties which has reduced hunger and famine - Current research to develop crops resistant to drought and flooding may help mitigate climate change - Understanding evolutionary theory allows biologists to devise strategies for pesticide application that minimize evolution of pest resistance
Modern Agriculture depends on Biology
59
-Biology helps us understand diseases and their causes, and develop treatments - Evolutionary principles help us to understand how diseased organisms evolve resistance to drugs - Development of Vaccines control major epidemics
Biology is the basis of practical medicine
60
Biologists are called to advise government agencies on many issues (ex. overfishing of bluefin tuna)
Biology can inform public policy
61
- Human activities are causing unprecedented rates of change in Earth's systems - Increasing atmospheric CO2 is responsible for climate warming, which is contributing to species extinction and spread of disease - Biodiversity helps us understand, enjoy, and appreciate our world - Biodiversity enriches our lives in many ways: enhancing our enjoyment of bird watching, gardening, hunting, and fishing, collecting mushrooms, hiking, camping, etc.
Biology is crucial for understanding ecosystems
62
The living together of two species in a prolonged and intimate relationship
Symbiosis
63
-All matter is composed of ______ -The smallest unit of a chemical element. Consists of a nucleus and one or more electrons
Atom(s)
64
Neglible Mass; negative charge
Electron
65
Have mass; positive charge; apart of the atomic nucleus
Proton
66
Have mass; no charge; apart of the atomic nucleus
Neutron
67
The quantity of measure of matter present; the greater the _____ the greater the quantity of matter.
Mass
68
In cells, the centrally located compartment of eukaryotic cells that is bounded by a double membrane and contains chromosomes. In the brain, an identifiable group of neurons that share common characteristics or functions
Nucleus
69
The mass of the proton serves as the standard unit of measure called ______. A measure for the mass of atoms equal to the atomic mass unit. 1.7 x 10^-24
Dalton
70
A fundamental substance that contains only one type of atom; a substance that can't be converted into a simpler substance by ordinary chemical means.
Element
71
The number of protons in the nucleus of an atom; also equals the number of electrons around the atom. Determines the chemical properties of an atom
Atomic Number
72
The arrangement of elements in order of increasing atomic number, with vertical groupings of similar valence electrons.
Periodic Table
73
The sum of the protons and neutrons in an atom's nucleus
Mass number
74
Forms of a given chemical element that have the same number of protons in their nuclei (and are in the same position on the periodic table) but differ in the number of neutrons.
Isotope
75
Carbon atoms in biological molecules are ____, but some are ___
12C;13C
76
The average of the mass of numbers of a representative sample of atoms of an element, with all the isotopes in their normally occurring properties
Atomic Weight
77
A radioactive isotope of an element. ex. carbon-14 and hydrogen-3. They give off energy in the form of alpha, beta, and gamma radiation. The atom is sometimes transformed into a different element.
Radioisotopes
78
The spontaneous disintegration of a substance with the emission of ionizing radiation
Radioactive decay
79
A chemical substance is made up of two or more atoms joined by covalent bonds or ionic bonds. A stable association of atoms.
Molecule
80
___________ in an atom determines how it combines with other atoms
Number of electrons
81
A region in space surrounding the atomic nucleus in which an electron is most likely to be found (90% of the time).
Orbital
82
The region surrounding the atomic nucleus at a fixed energy level in which electrons orbit. The farther from the nucleus the more energy. - First Shell: 1 orbital; holds 2 electrons - Second Shell: 4 orbitals; holds 8 electrons - Additional Shells: 4 orbital; holds 8 electrons
Electron Shell
83
-_____________ atoms have unpaired electrons in their outermost shell; can share electrons, or lose or gain electrons
Reactive
84
Tendency of atoms to form stable molecules resulting in a full valence shell | 8
Octet Rule
85
The outermost energy shell of an atom, contains the valence electrons involved in chemical reactivity. The outermost shell determines how the atom behaves; if full it's stable
Valence Shell
86
An attractive force stably linking two atoms to form molecules | C____________
Chemical Bonds
87
A chemical bond is based on the sharing of electrons between two atoms. The outer shells are filled. Each atom contributes one member of each electron pair. - Single, sharing 1 pair of electrons - Double, sharing 2 pairs of electrons - Triple, sharing 3 pairs of electrons Bond energies are higher in multiple ___________
Covalent Bonds
88
A substance is made up of atoms of more than one element. Made up of many units, as in the compound eyes of arthropods.
Compound
89
The sum of the atomic weights of the atoms in a molecule.
Molecular Weight
90
The tendency of an atom to attract electrons when it occurs as a part of a compound.
Electronegativity
91
A covalent bond between atoms that has equal sharing of electrons (atoms have similar electronegativity)
Nonpolar Covalent Bonds
92
A covalent bond in which the electrons are drawn to one nucleus more than the other, resulting in an equal distribution of charge. (atoms have very different electronegatively; positive one end and negative other end)
Polar Covalent Bonds
93
Referring to a molecule with separate electric charges at two ends, or poles; water molecule
Polar
94
An electrically charged particle that forms when an atom gains or loses one or more electrons
Ions
95
An ion with one or more positive charges
Cation
96
An ion with a negative charge
Anion
97
Groups of Covalently bonded atoms that carry an electric charge (NH4+)
Complex Ion
98
An electrostatic attraction between positively and negatively charged ions. A complete transfer of electrons may occur, resulting in two ions with full outer shells. (ex. Salts) Ions interact with polar molecules (e.g. salts dissolving in water)
Ionic Bonds
99
A weak electrostatic bond which arises from the attraction between the slight positive charge on a hydrogen atom and a slight negative charge on a nearby oxygen or nitrogen atom
Hydrogen Bonds
100
Water loving; Having an affinity for water
Hydrophilic
101
Water hating; Having no affinity for water; Uncharged and nonpolar groups of atoms are ___________
Hydrophobic
102
A molecule containing only hydrogen and carbon atoms; Nonpolar molecule and hydrophobic
Hydrocarbons
103
A weak chemical interaction is caused when molecules that are not attracted to water interact to exclude water.
Hydrophobic Interactions
104
Weak attractions between atoms resulting from the interaction of the electrons of one atom with the nucleus of another. This type of attraction is about one-fourth as strong as a hydrogen bond. Can be substantial when summed over a large molecule.
Van der WaalsForces
105
The changes in the composition or distribution of atoms of a substance, with consequent attractions in properties. Occurs when atoms collide with enough energy to combine or change their bonding patterns
Chemical Reactions
106
A chemical substance that enters into chemical reaction with another substance
Reactant
107
A molecule that results from the completion of a chemical reaction
Product
108
A reaction in which one substance transfers one or more electrons to another substance. ex. Combustion. They both take place at the same time - The electron acceptor (oxidizing agent) gains electrons and becomes reduced (oxygen) - The electron donor (reducing agent) loses electrons and becomes oxidized (propane)
Oxidation-Reduction (Redox) Reactions
109
The capacity to do work or move matter against an opposing force. The capacity to accomplish change in physical and chemical systems. Usually changes during chemical reactions.
Energy
110
The amount of energy that must be absorbed by a gram of a substance to raise its temperature by one-degree centigrade. By convention, water is assigned a specific heat of one. Water has a high specific heat capacity because of hydrogen bonding.
Specific Heat
111
The energy that must be supplied to convert a molecule from a liquid to a gas at its boiling point
Heat of Vaporization
112
The transition of water from liquid to the gaseous phase
Evaporation
113
The tendency of molecules (or any substance) to stick together. Hydrogen bond between water molecule causes them to stick together.
Cohesion
114
The binding of one cell or substance to another. Attraction of water molecules to other molecules of a different type.
Adhesion
115
A liquid ( the solvent) and it's dissolved solutes.
Solution
116
A substance that is dissolved in a liquid (solvent) to form a solution.
Solute
117
The liquid in which a substance (solute) is dissolved to form a solution.
Solvent
118
A quantity of a compound whose weight in grams is numerically equal to its molecular weight expressed in atomic mass units. Avagoadro's number of molecules: 6.023 x 10^23 molecules.
Mole
119
The number of atoms in a mole (weighed out in grams) of a substance calculated to be _____________
6.023 x 10^23; Avagadro's Number
120
A substance that can release a proton (H+) in solution
Acids
121
A substance that can accept a hydrogen ion (H+) in solution.
Base
122
A chemical transformation that can occur in either direction, so that reactants become products and vice versa
Reversible Reaction
123
The negative logarithm of the hydrogen ion concentration; is a measure of the acidity of a solution. A solution with a __ = 7 is said to be neutral; ___ values higher than 7 characterize basic solutions, while acidic solutions have __ values less than 7.
pH
124
A substance that can transiently accept or release hydrogen ions and thereby resist changes in pH
Buffer
125
The principle that the rate of a chemical reaction is directly proportional to the concentrations of the reacting substances.
Law of Mass Actions
126
A large molecule made up of similar or identical subunits called monomers. Proteins, Carbohydrates, and Nucleic Acids are _______
Polymers
127
A small molecule, two or more of which can be combined to form oligomers (consisting of a few __________) or polymers (consisting of many ___________).
Monomers
128
A giant (molecular weight > 1,000) polymeric molecule. The ___________ are proteins, polysaccharides, and nucleic acids. Large lipids are also _______
Macromolecules
129
A characteristic combination of atoms that contributes specific properties (such as charge or polarity) when attached to larger molecules (e.g., carboxyl group, amino group). A group of species that function in similar ways, whether or not they use the same resources.
Functional Group
130
Molecules consisting of the same numbers and kinds of atoms, but differing in the bonding pattern by which the atoms are held together.
Isomers
131
Molecules made up of the same kinds and numbers of atoms, in which the atoms are bonded differently.
Structural Isomers
132
In molecules with a double bond, (typically between two carbon items), identifies on which side of the double bond similar atoms or functional groups are found. If they are on the same side, the molecule is a ___ isomer, in a _____ isomer, similar atoms are on opposite sides of the double bond
Cis;trans Isomer
133
Two molecular isomers that are mirror images of each other
Mirror Isomers
134
A chemical reaction in which two molecules become connected by a covalent bond and a molecule of water is released. Energy is used to make covalent bonds between monomers to make a polymer; a water molecule is removed or produced.
Condensation Reaction
135
A chemical reaction that breaks a bond by inserting the components of water. A polymer is broken down into monomers; energy is released and water is consumed.
Hydrolysis Reaction
136
A long-chain polymer of amino acids with 20 different common side chains. Occurs with its polymer chain extended in fibrous ________, coiled into a compact macromolecule in enzymes and other globular ________. The component amino acids are encoded in the triplets of messenger RNA, and proteins are the products of genes.
Proteins
137
An organic compound containing both NH2 and COOH groups. Proteins are polymers of _______________
Amino Acid
138
The distinguishing group of atoms of a particular amino acid. Also called a side chain
R group (side chain)
139
The covalent bond between two sulfur atoms (-S--S-) linking to molecules or remote parts of the same molecule
Disulfide Bridge
140
A short polymer of 20 or fewer amino acids. Also called and
Peptides;Oligopeptide
141
A large molecule made up of 20 or more amino acids joined by peptide bonds. Large ________ are called proteins.
Polypeptide
142
The bond between amino acids in a protein; formed between a carboxyl group and amino group with the loss of water molecules.
Peptide Bond
143
The specific sequence of amino acids in a protein.
Primary Sequence
144
In a protein, the localized regularities of structure, such as the ⍺ helix and the β pleated sheet. Things that affect this structure: High Temp, pH changes, High Concentration, and Non-polar substances.
Secondary Structure
145
A prevalent type of secondary protein structure; a right-handed spiral.
⍺ (alpha) helix
146
A type of protein secondary structure; results from hydrogen bonding between polypeptide regions running antiparallel to each other.
β (beta) pleated sheet
147
In a protein, the relative locations in three-dimensional space of all the atoms in the molecule. The overall shape of protein. Things that affect this structure: High Temp, pH changes, High Concentration, Non-polar substances.
Tertiary Structure
148
The loss of activity of an enzyme or nucleic acid molecule as result of structural changes induced by heat or other means
Denatured
149
Aggregates of many individual molecules of a protein that self-assemble into large fibers. Associated with human diseases, such as Alzheimer's disease.
Amyloids
150
The specific three-dimensional arrangement of protein subunits. Results from interaction of subunits by hydrophobic interactions, van der Waals forces, ionic attractions, and hydrogen bonds.
Quaternary Structure
151
A protein that guards other proteins by counteracting molecular interactions that threaten their three-dimensional structure.
Chaperones
152
Organic Compounds containing carbon, hydrogen, and oxygen in the ratio 1:2:1. Common examples are sugars, starch, and cellulose.
Carbohydrates
153
A simple sugar sugar. Oligosaccharides and Polysaccharides are made up of ________________.
Monosaccharides
154
A carbohydrate made up of two monosaccharides
Disaccharides
155
A polymer containing a small number of monosaccharides
Ogliosaccharides
156
A macromolecule is composed of many monosaccharides. Cellulose and Starch
Polysaccharides
157
A sugar containing five carbon atoms.
Pentoses
158
A sugar containing six carbon atoms
Hexoses
159
A bond between carbohydrate (sugar) molecules through an intervening oxygen atom
Glycosidic Bonds
160
An energy-storage polysaccharide found in animals and fungi; a branched-chain polymer of glucose, similar to starch.
Glycogen
161
A straight-chain polymer of glucose molecules, used by plant as strcutual supporting material.
Cellulose
162
Non-polar, hydrophobic molecules that include fats, oils, waxes, steroids, and the phospholipids that make up biological membranes.
Lipids
163
A simple lipid in which three fatty acids are combined with one molecule of glycerol
Triglycerides
164
A triglyceride that is solid at room temperature. Adipose tissue, one type of connective tissue.
Fats
165
A triglyceride that is liquid at room temperature
Oils
166
A three-carbon alcohol with three hydroxyl groups; a component of phospholipids and triglycerides
Glycerol
167
A molecule that is made up of a long nonpolar hydrocarbon chain and a polar carboxyl group. Found in many lipids
Fatty Acid
168
A condensation (water-releasing) reaction in which the carboxyl group of fatty acid reacts with the hydroxyl group of an alcohol. Lipids, including most membrane lipids, are formed in this way.
Ester linkage
169
A fatty acid in which all the bonds between carbon atoms in the hydrocarbon chain are single bonds- that is, all the bonds are saturated with hydrogen atoms.
Saturated Fatty Acids
170
A fatty acid whose hydrocarbon chain contains one or more double bonds. This results in kinks that prevent packing (plant oils; liquid at room temperature)
Unsaturated Fatty Acids
171
An unsaturated fatty acid with a 2-carbon double bond at position 3 in the fatty acid chain. They protect against heart disease and stroke
Omega-3 fatty acids
172
Of a molecule, having both hydrophilic and hydrophobic regions.
Amphipathic
173
A lipid containing a "head" phosphate group that is hydrophilic and a "tail" that are fatty acid chains that are hydrophobic: an important constituent of cellular membranes. they also have glycerol and the two fatty acid tails can be either saturated or unsaturated.
Phospholipids
174
A structure that is two layers in thickness. In biology, most often refers to the phospholipid ______ of membranes.
Bilayer
175
Tightly together their hydrophilic, phosphate-containing "heads" face outward. Also called a lipid bilayer.
Phospholipid Bilayer
176
Lipids packages inside a covering of protein, which allows them to be circulated in the blood.
Lipoproteins
177
Light-absorbing pigment, e.g. β - carotene traps light energy for photosynthesis. In humans, β - carotene breakdown into Vitamin A.
Carotenoids
178
Multiple rings share carbons. Cholesterol is important in membranes; other _________ are hormones.
Steroids
179
Long-chain alcohol bound to an unsaturated fatty acid
Waxes
180
A polymer made up of nucleotides, specialized for the storage, transmission, and expression of genetic information. DNA and RNA are ___________
Nucleic Acids
181
The fundamental hereditary material of all living organisms. In eukaryotes, stored primarily in the cell nucleus. A nucleic acid using deoxyribose rather than ribose. Two strands form a double helix. All ___ molecules have the same structure. Genetic information is carried in the sequence of base pairs. ___ can reproduce itself (replication).
DNA (Deoxyribonucleic Acid)
182
Often single-stranded nucleic acid whose nucleotides use ribose rather than deoxyribose and in which the base uracil replaces thymine found in DNA. Serves as genome for some viruses. Single-stranded. Base pairing can occur between different regions of the molecule which results in 3-D structure
RNA (Ribonucleic Acid)
183
In nucleic acids, the purine or pyrimidine that is attached to each sugar in the sugar-phosphate backbone.
Nitrogenous Base
184
A nucleotide without the phosphate group; a nitrogenous base attached to a sugar.
Nucleoside
185
One of the two types of nitrogenous bases in nucleic acids; cytosine, thymine, and uracil. Single ring
Pyrimidine
186
One of the types of nitrogenous bases in nucleic acids; adenine and guanine. Fused double ring
Purines
187
The connecting in nucleic acid strand is formed by linking two nucleotides. Two covalent bonds through a phosphate group link the sugars of adjacent nucleotides.
Phosphodiester Bond
188
A nitrogen-containing base found in nucleic acids, ATP, and other Compounds
Adenine (A)
189
A nitrogen-containing base found in DNA and RNA
Cytosine (C)
190
A nitrogen-containing base found in DNA, RNA, and GTP
Guanine (G)
191
A nitrogen-containing single-ringed base found in DNA
Thymine (T)
192
A pyrimidine base found in nucleotides of RNA
Uracil (U)
193
The A-T (or A-U), T-A (or U-A), C-G, and G-C parings of bases in double-stranded DNA< in transcription, and between tRNA and mRNA.
Complementary Base Pairing
194
The creation of a new strand of DNA which DNA polymerase catalyzes the exact reproduction of an existing (template) strand of DNA.
DNA replication
195
The synthesis of RNA using one strand of DNA as a template
Transcription
196
The synthesis of a protein (polypeptide). Takes place on ribosomes, using the information encoded in the messenger RNA/
Translation
197
The process of transcription and translation into a protein of the information (nucleotide sequence) contained in a gene.
Gene Expression
198
A unit of hereditary, Used here as the unit of genetic function that carries the information for a polypeptide or RNA.
Genes
199
The idea is that under ordinary circumstances, living organisms can form from nonliving materials.
Spontaneous Generation
200
________ and _____ (1950s) experiment with reconstructing those primitive conditions using gases thought to be in the early atmosphere: H2, NH3, CH4, H2O
Miller and Urey
201
The theory that life originated through the chemical transformation of inanimate substances.
Chemical Evolution
202
The simplest structural unit of a living organism. In multicellular organisms, many individual ___ serve as the building blocks of tissues and organs.
Cells
203
A phospholipid bilayer forming a barrier that separates the internal contents of a cell from the nonbiological environment or encloses the organelles within a cell. The membrane regulates the molecular substances entering or leaving a cell or organelle.
Membrane
204
A self-organized sphere of lipids proposed as a stepping-stone to the origin of life.
Protocells
205
The theory that all cells are the basic structural and physiological units of living organisms, and that all cells come from pre-existing cell
Cell theory
206
For any cell, organism, or geometrical, solid, the ratio of__________________; this is an important factor in setting an upper limit of the size a cell or organism can attain
Surface area to volume ratio
207
The membrane that surrounds the cell, regulating the entry and exit of molecules and ions. Every cell has a cell membrane. The ______________ is composed of phospholipids.
Cell membrane
208
The fluid portion of the cytoplasm, excluding organelles, and other solids
Cytosol
209
The contents of the cell excluding the nucleus
Cytoplasm
210
Unicellular organism that do not have nuclei or other membrane-enclosed organelles. Includes bacteria and archaea.
Prokaryotes
211
Organism whose cells contained their genetic material inside the nucleus, includes all life, other than viruses, archaea, bacteria.
Eukaryotes
212
Any of the membrane enclosed structures with a eukaryotic cell. Examples include the nucleus, endoplasmic reticulum, and mitochondria.
Organelles
213
In cells, the centrally located compartment of eukaryotic cells that is bounded by a double membrane, and contains the chromosomes
Nucleus
214
The region that harbors, the chromosomes of a prokaryotic cell. Unlike the eukaryotic nucleus, it is not bounded by a membrane.
Nucleoid
215
A small particle in the cell that is the site of protein synthesis
Ribosome
216
A relativel structure, that encloses cells of plants, fungi, many protists, and most prokaryotes, and which gives the cells their shape and limits their expansion in the hypotonic media.
Cell wall
217
In organelles that have two membranes, such as chloroplast and mitochondria, the ________________ encloses the entire organelle
Outer Membrane
218
In some prokaryotes, dense layer of polymers that surrounds the cell wall
Capsule
219
A membrane that is enclosed within a membrane bound organelle examples include membranes around thylakoids and chloroplast, and the mitochondrial membrane
Internal membrane
220
A whip-like appendage propels cells. Prokaryotic differ greatly from those found in eukaryotes. ________ are about 100 to 200 µm and occur singly or in pairs; composed of microtubules, which are arranged in a 9+2 array.
Flagella
221
A structure that links prokaryotes cells at the beginning of conjunction
Pili
222
The network of microtubules and microfilaments that give a eukaryotic cell its shape, and its capacity to arrange its organelles and to move. Support cells, hold cells and organelles together, moves organelles, moves cytoplasm, and interacts with extracellular structures.
Cytoskeleton
223
A small, generally spherical body found within the nucleus of eukaryotic cells, the site of synthesis of ribosomal RNA
Nucleolus
224
A double membrane that includes the cell nucleus
Nuclear envelope
225
Nucleic acid protein complex that makes up eukaryotic chromosomes
Chromatin
226
In bacteria and viruses, the DNA molecule that contains most or all of the genetic information of the cell or virus. And eukaryotes, a structure, composed of DNA and proteins, that bears part of the genetic information of the cell.
Chromosome
227
System of intracellular membranes, that exchange material with one another, consisting of the Golgi apparatus, endoplasmic reticulum, and lysosomes when present
Endomembrane system
228
Within the cytoplasm, a cell membrane-enclosed compartment that is associated with other organelles; the Golgi apparatus is one example
Vesicles
229
A system of membranous tubes and flatten sacks in the cytoplasm of eukaryotes. Exist in two forms: rough ER, studied with ribosomes; and smooth, ER, lacking ribosomes.
Endoplasmic reticulum
230
The portion of the endoplasmic reticulum, whose outer surface has attached ribosomes
Rough endoplasmic reticulum
231
The portion of the endoplasmic reticulum that lacks ribosomes, and has a tubular appearance
Smooth endoplasmic reticulum
232
A system of concentrically folded membranes found in the cytoplasm of eukaryotic cells; functions in secretion from the cell by exocytosis. The Golgi apparatus receives protein containing vesicle, modifies, concentrates, and packages proteins, add carbohydrates to proteins and modifies other carbohydrates, it's where some polysaccharides or plant cells are made, and cuts certain large precursor proteins into smaller pieces.
The Goldie apparatus
233
The lysosome before fusion of an endosome
Primary lysosomes
234
The process of cell uses its cell membrane to enclose and engulf a macromolecule or particle in the extracellular environment
Phagocytosis
235
A membrane organelle formed by the fusion of a primary lysosome with a figure zone, in which molecules taken up by phagocytosis or hydrolyzed into monomers
Secondary lysosome
236
A process by which a vesicle in a cell fuses with the cell membrane and releases its contents to the outside
Exocytosis
237
The programmed destruction of cell's components
Autophagy
238
A class of human diseases, invariably harmful or fatal, that are caused by the failure of lysosomes to digest specific cellular components. An example is Tay-Sachs disease.
Storage Diseases
239
Energy-generating organelles in eukaryotic cells that contain the enzyme of the citric acid cycle, the respiratory chain, and oxidative phosphorylation.
Mitochondria
240
The catabolic pathways by which electrons are removed from various molecules and passed through intermediate electrons carries O2, generating H2O and releasing energy; Highly exergonic; drives endergonic formation of many ATP molecules
Cellular Respiration
241
An organelle bounded by a double membrane containing the enzymes and pigments that perform photosynthesis.____________ occur only in eukaryotes.
Chloroplasts
242
A flattened sac within a chloroplast. _________ membranes contain all of the chlorophyll in a plant in addition to the electron carries of phosphorylation. _____________ stack to form grana
Thylakoids
243
An organelle that houses reactions in which toxic peroxides are formed and then converted into water.
Peroxisome
244
A plant organelle in which stored lipids are converted to carbohydrates.
Glyoxysomes
245
A membrane-enclosed organelle in plant cells that can function for storage, water, concentration for turgor, or hydrolysis of stored macromolecules. Provides structure, Contains pigments that help the plant with reproduction, and is used for storage.
Vacuoles
246
In eukaryotic cells, a fibrous structure is made up of actin molecules monomers. ____________________ play roles in the cytoskeleton, in cell movement, and in muscule contraction.
Mircofilaments
247
A protein that makes up the cytoskeleton, microfilaments, and eukaryotic cells, and one of the two contractile proteins in muscle
Actin
248
A part of the cytoskeleton that includes filaments, ______________ in diameter between microtubules and microfilaments
Intermediate filaments
249
Tubular structures found in the centrioles, spindle apparatus, cilia, flagella, and cytoskeleton of eukaryotic cells. ___________ roles in the motion and maintenance of shape of eukaryotic cells.
Microtubules
250
A protein that polymerizes to form microtubules
Tubulin
251
Specialized proteins that use energy to change shape, and move cells or structures within cells | Membrane protein involved in the movement of ions, small molecules;move
Membrane transport proteins
252
A type of protein that binds to microtubules and also vesicles. By moving along the microtubules, _________ act as motor proteins to move attached vesicles. Moves materials to the plus end
Kinesins
253
A type of protein that binds to microtubules and causes, bending in cilia and flagella. Move materials towards the minus end.
Dyneins
254
Hair like organelles used for locomotion by many unicellular organisms, and for moving water and mucus by many multicellular organisms, generally shorter than flagella; ____ are about 0.25 µm in length; composed of microtubules, arrange in a 9+2 array
Cilia
255
In Plant cells, membrane line channels that extend through the cell walls and connect the cytoplasm of adjacent cells
Plasmodesma
256
A material of heterogeneous compositions surrounding cells, and performing many functions, including adhesions of cells.
Extracellular matrix
257
A fiber protein found exclusively in bone and connective tissue
Collagen
258
A glycol protein, containing a protein core with attached long, linear carbohydrate chains
Proteoglycans
259
A living together of two or more species in a prolonged and intimate state
Symbiosis
260
The theory that the eukaryotic cell evolved via the engulfing of one prokaryotic cell by another
The theory of Endosymbiosis
261
A molecular model for the structure of biological membranes, consisting of fluid phospholipid bilayer in which suspended proteins, are free to move in the plane of the bilayer
Fluid mosaic model
262
- the cell membrane is full of motion - cholesterol can be added to decrease the fluidity of the membrane - cells can also change the saturation of the fatty acids on the fossil lipids to increase or decrease _____________ - fluid: unsaturated, hydrocarbon tails with kinks - viscous: saturated hypocarbon tails that are straight
Membrane Fluidity
263
__________ is a flat (planer) hydrophobic molecule. And eukaryotic cells membrane prevent "close packing" of phospholipids. At colder temperatures, membrane still maintain fluid like motion. Temperature adaptation, and some animals involves adjusting the amount of __________ in their membranes.
Cholesterol
264
Proteins that are at least partially embedded in the cell membrane
Integral membrane proteins
265
Proteins associated with, but not embedded within the cell membrane
Peripheral membrane proteins
266
Proteins that are associated with the membrane by covalent attachments to lipids | attachments
Anchor membrane proteins
267
An integral membrane protein that spans the phospholipid bilayer
Transmembrane protein
268
A protein region, rich in hydrophobic amino acids, that spans the phospholipid bilayer
Transmembrane domains.
269
A lipid which sugars are attached to
Glycolipid
270
Protein to which sugars are attached
Glycoprotein
271
The specific binding of cells to one another mediated by membrane proteins or carbohydrates | cell binding, the r_____
Cell recognition
272
The binding of one cell to another, often mediated by noncovalent forces
Cell adhesion
273
Pertaining to adhesion of cells of the same type
Homotypic
274
Pertaining to the binding of the same types of cells, due to the interaction of the same molecules on the cell surfaces
Heterotypic
275
Specialized structures associated with the cell membranes of epithelial cells. Some contribute to cell adhesion, others to intercellular communication. | together;adhesion
Cell junctions
276
Adjunction, epithelial cells in which there is no gap between adjacent cells
Tight junctions
277
And adhering junction between animal cells | a structure; somes
Desmosomes
278
A 2.7 nm gap between cell membranes of two animal cells, spanned by proteins protein channels. ___________ allow chemical substances or electrical signals to pass from cell to self.
Gap junctions
279
In animals, a transmission protein that mediates the attachment of epithelial cells to the extracellular matrix | In______
Integrin
280
The characteristics of membranes that allows certain substances to pass through, while other substances are excluded
Selective permeability
281
Diffusion across a membrane; may or may not require a channel or carrier proteins. Occurs by diffusion.
Passive transport
282
The dependent transport of a substance across a biological membrane against a concentration gradient that is, from a region of low concentration (of that substance) to one high concentration
Active transport
283
A difference in concentration of an ion or other chemical substance from one location to another, often across a membrane
Concentration gradient
284
The random movement of molecules, or other particles, resulting in the even distribution of the particles, when no barriers are present; solute moves from areas of high concentration to areas of low concentration movement continues until equilibrium has been reached
Diffusion
285
When the net movement of molecule stops, because the concentration of solute is equal on both sides of the membrane
Equilibrium
286
- the size and mass of the molecules are ions - the temperature of the solution - the density of the solution - the concentration gradient in the system - the area across which a substance diffuses and the distance it diffuses
Things that determine how fast a substance diffuses
287
Diffusion that does not involve a direct input of energy or assistance by carrier proteins
Simple diffusion
288
The movement of water across differentially permeable membrane, from one region to another region where the water potential is more negative
Osmosis
289
-small hydrophobic molecules, O2, CO2, N2, benzene, and small and charged polar molecules, H2O, glycerol, and ethanol: are able to pass through the lipid bilayer - large, uncharged, polar molecules, amino acids, glucose, nucleotides, and ions: H+, Na+, HCO3-, K+, Ca 2+, Cl-, Mg 2+: are unable to pass through
Membrane permeability
290
Having the same solute concentration; said of two solutions
Isotonic
291
Having a lower solute concentration; said of one solution, and comparing it with another
Hypotonic
292
Having a higher solute, concentration: set of one solution, and comparing it with another
Hypertonic
293
An integral membrane protein, that forms in aqueous passageway across the membrane in which it is inserted in which specific solutes may pass
Channel proteins
294
Proteins that bind another substance to transport it from one location to another; carriers bind, small molecules, and transport them across membranes
Carrier proteins
295
Passive movement through a membrane involving specific carrier proteins; is not proceed against the concentration gradient
Facilitated diffusion
296
And integral membrane protein that allows ions to diffuse across the membrane in which it's embedded
Ion channels
297
A membrane protein, that changes, its three-dimensional shape, and therefore its ion conduct, in response to a stimulus. When open, it allows specific ions to move across the membrane.
Gated channel
298
Any molecule that binds to a receptor site of another
Ligand
299
Transport protein in plant and animal cell membranes through which water passes in osmosis.
Aquaporins
300
A membrane transport protein that carries a single substance in one direction
Uniporter
301
A membrane transport protein that carries two substances in the same direction
Symporter
302
A membrane transport protein that moves one substance in one direction, and another in the opposite direction
Anti-Porter
303
Membrane proteins that transport, two substances across a membrane, often with the transport of one energetically driving the transport of another | two
Coupled transporters
304
Actor transport, in which ATP is hydrolyzed, yielding, the energy required to transport an ion or molecule against its concentration gradient
Primary active transport
305
The form of active transport, that does not use ATP as an energy source; rather, transport is coupled to ion diffusion down a concentration gradient, establishing a primary active transport. Also called transport.
Secondary active transport (co-transport)
306
A process by which lipids or solid particles are taken up by so through invagination of the cell membrane
Endocytosis
307
Mitosis by a cell of liquid containing dissolved substances, within vesicles
Pinocytosis
308
Endocytosis initiated by macromolecular binding to specific membrane receptor
Receptor-mediated endocytosis
309
A protein that combine to a specific molecule, or detect a specific stimulus, within the cell or the cells external environment
Receptor proteins
310
The series of biochemical steps, whereby a stimulus to a cell (such as a hormone or nerve transmitter binding into a receptor) is translated into a response of the cell | st pathway
Signal transaction pathway
311
Unicellular and multicellular organisms must have ____________. It's important for coordinating multiple cell actions within the organism. ____________________ is usually done by chemical signaling: examples, fighter flight response, hunger, satiation, embryonic development.
Cell communication
312
1. Synthesis of signaling molecule. 2. Release of signaling molecule. 3. transport of signaling molecule to target cell 4. Reception of signal by target cell 5. Transduction of signal within target cell 6. Response by the target sell.
Steps in cell communication
313
_________________ are any molecules that can be used for communication. Can be proteins, small polypeptides, gases, and steroids (hydrophobic), which are also called ligands.
Signaling molecules
314
Can diffuse across the cell membrane, ex. steroid hormones- usually has an internal receptor; Ligand
Small hydrophobic molecules
315
Cannot pass through the membrane. ex. Gross factors - must have an external receptor; Ligand
Water soluble (hydrophilic) molecules
316
Cannot pass the cell membrane. ex. prostaglandins - must have an external receptors | ______loving
Large lipophilic hormones
317
Pertaining to a chemical signal that binds to add effect the cell that makes it
Autocrine
318
Pertaining to a chemical signal that binds to, and affects only cells adjacent to it, and which it is in direct contact
Juxtacrine
319
Pertaining to a chemical signal that binds to, and affects nearby cells
Paracrine
320
A chemical signal produced in minute amounts at one site in a multicellular organism and transported to another site where acts on target cells
Hormones
321
Interactions between signal transduction pathways | between
Cross talk
322
A molecule in the target cell that binds to a ligand with high affinity and high specificity
Receptor
323
Strength of the bond
Affinity
324
A ______________________ Larger polar, Ligands cannot cross the lipid bilayer example insulin | type of something;M____ R_____
Membrane receptors
325
Small or nonpolar Ligands can diffuse across the nonpolar phospholipid bilayer of the cell membrane and enter the cell example estrogen. The receptor that binds its ligand inside the cell.
Intracellular receptors
326
An equilibrium constant measuring the tendency of two substances that are bound together to separate into two smaller components. In cell signaling, the two substances are a ligand and a receptor.
Dissociation constant
327
A chemical substance that illicit a specific response in a cell or tissue example neurotransmitter
Agonist
328
Chemical substance that blocks the other action of a biochemical substance
Antagonist
329
An enzyme that catalyzes the addition of a phosphate group from ATP to a target protein | k______
Protein kinases
330
A class of receptors that change configuration upon ligand binding such that a G protein binding site is exposed on the cytoplasmic domain of the receptor, initiating a signal transduction pathway | c_______-;together
G-Protein coupled receptors
331
A membrane protein involved in signal transduction; characterized by binding GDP or GTP
G Protein
332
In cell signaling, a protein responsible for the cellular response to a signal transduction pathway
Effector protein
333
A compound, such as cAMP, that is released within a target cell after a hormone (the first messenger) has bound to a surface receptor on the cell; the ________________ triggers further reactions within the cell
Second Messengers
334
A series of reactions in response to the molecular signal, in which a series of protein kinases active in sequence, amplifying the signal at each step
Protein Kinases cascade
335
The signal that stimulates mitosis
Mitogens
336
This is a common second messenger. The enzyme that catalyzes information of ________ from ATP (Adenylyl cyclase) is located on the cytoplasmic side of the plasma membrane. Binds to ion channels, and many kinds of sensory cells and opens the channel. Binds to protein kinases and or phosphorus and cytoplasm this exposes, the active site and a protein kinase cascade
Cyclic AMP
337
A phospholipid in the cell membrane that is involved in cell, signaling after a ligand binds to a nearby receptor
Phosphatidyl Inositol-biphosphate
338
In hormone action, the second messenger produced by hydrolytic removal of the head group of certain Phospholipids
Diacyglyceride (DAG)
339
Intracellular second messenger, derived from membrane phospholipids | I___
Inositol triphosphate (IP3)
340
Glycogen synthase, which catalyzes the joining of glucose molecules to synthesize the energy storing molecule glycogen, it is activated when a phosphate group is added to it by protein kinase A. thus the epinephrine signal prevents glucose from being stored in the form of glycogen
Inhibition
341
Phosphorylase kinase is activated when a phosphate group is added to it. It is a part of protein kinase cascade, that ultimately leads to the activation of glycogen phosphorylation cascade, that ultimately leads to the activation of glycogen phosphorylase, another key enzyme in glucose metabolism. This enzyme results in the liberation of glucose molecules from glycogen.
Activation
342
In a gap junction, protein channel linking adjacent animal cells
Connexons
343
In plants, the tubule inside a plasmodesma, through which small metabolites and ions move between plant cells
Desmotubule
344
_____________ is a relay of a signal from receptors to target molecules often leads to a phosphorylation cascade and ultimately cellular response. One molecule can create a large response, and one molecule can create a diversity of responses.
Signal transduction
345
Different cells can respond differently to the same signal. Signals can be amplified. Signal can overlap or influence one another. A signal could have multiple effects within the same cell. A few signaling and receptor molecules can accomplish a symphony of effects.
Why so many different steps?
346
The signals after the first messenger. The _______________________ is released into the cytoplasm after signal bines to receptor second message affect. Many processes in the cell. Usually small diffusible molecules include cAMP,CA2+ NO,IP3 and DAG.
Secondary messengers
347
A large class of receptor proteins that have enzymatic activity; can initiate 10 or more transduction pathways ;help cells regulate in coordinate cell growth and reproduction
Tyrosine Kinase receptor
348
Overexpression of RTK receptor leads to continuous binding of growth factors. The cell cycle is continuously turned on". Cause aggressive breast, tumor growth
Breast cancer
349
Channel proteins that allow ions to enter a cell. Signals activate receptors to open and can be chemical Ligand such as hormones. Sensory stimuli, such as light, or electric charge differences in acetylcholine receptor on muscle cell is a __________________
Gated ion channel receptors
350
____________ program cell death. It is preferred method of cell death and is used throughout the lifespan of multicellular organisms. This signal can be intrinsic (directed from within the cell) extrinsic (directed by other cells)
Apoptosis
351
Cells receive apoptoic signal. Caspasea (CED) begins the process of breaking down the cell. Blebs form. Cell release a signal to invoke phagocytosis.
Apoptosis pathway
352
Dramatic changes in bacterial activity once their numbers reach a threshold or quorum
quorum sensing
353
Species of strain, specific, signaling molecules or more general signals. Can result in light edition, secretion of proteins that allow for attack of host or production, or secretion of biofilms.
Bacterial communication
354
Energy not doing work, but with the potential to do so, such as the energy stored in chemical bonds
Potential energy
355
The energy associated with movement
Kinetic energy
356
A synthesis reaction in which complex molecules are broken down into simpler ones and energy is released.
Catabolic reactions
357
A synthetic reaction in which simple molecules are linked to form more complex once; requires an input of energy and captures it in the chemical bonds that are formed. They build large molecules from smaller ones.
Anabolic reactions
358
Laws, derived from studies of the physical properties of energy and the ways energy interacts with matter. 1. Energy in the universe is constant. Energy can be transferred and transformed, but it cannot be created or destroyed 2. Every energy transfer transformation increases the entropy of the universe. When energy is converted from one form to another, some of the energy becomes unavailable to do work.
Laws of thermodynamics
359
The total energy of a system
Enthalpy (H)
360
Energy is available for doing useful work, after allowance has been made for the increase or decrease of disorder
Free energy (G)
361
Referring to a chemical reaction in which the products of the reaction have lower free energy, then the reactants, resulting in a release of free energy; catabolism; complexity, decreases (generates disorder)
Exergonic reactions
362
Referring to a chemical reaction in which the products have higher free energy than the reactants, thereby requiring free energy input to occur. Anabolism; complexity (order increases).
Endergonic reactions
363
A state of Nob net charge, occurring when the forward and reverse reactions in a system take place at the same time
Chemical equilibrium
364
A test tube or similar vessel, in which the contents of cells are at least in studied, after the cells are lysed by the destruction of the integrity of the cell membrane
Cell free system
365
The energy associated with the chemical reaction that can be used to do work. The sum of the entropy and the product of temperature and entropy.
Standard free energy
366
In chemistry of substance that speeds of a reaction without being permanently, altered itself
Catalyst
367
Catalytic protein that speeds up a biochemical reaction
Enzymes
368
An enzyme, catalyzed reaction, the reactive condition of the substrate, after there has been sufficient input of energy (activation energy) to initiate the reaction | thisis a state
Transition state
369
The energy barrier that blocks the tendency for a chemical reaction to occur
Activation energy (EA)
370
The molecules on which an enzyme exerts catalytic action
Substrates
371
The region of an enzyme, or ribosome, where the substrate binds, and where catalysis occurs
Active site
372
Intermediate in an enzyme catalyzed reaction; consist of an enzyme, bound to its substrates. Held together by hydrogen bonds. E + S = ES= E + P
Enzyme substrate complex (ES)
373
A change in shape of an enzyme caused by binding to its substrate that exposes the active site of an enzyme
Induced fit
374
An RNA molecule with catalytic activity
Ribozymes
375
A non-protein carbon containing molecule that is required for the action of one or more enzymes
Coenzyme
376
The scientific study of an organism as an integrated and interacting system of genes, proteins, and biochemical reactions
Systems biology
377
A non-substrate that inhibits the activity of an enzyme by binding to its active site, preventing substrate binding
Competitive inhibitor
378
An enzyme inhibitor that bind the enzyme, substrate complex, preventing the complex from releasing products
Uncompetitive inhibitor
379
A non-substrate that inhibits the activity of an enzyme by binding to a site other than it's active site
Noncompetitive inhibitor
380
Regulation of the activity of a protein (usually an enzyme) by binding of a non-substrate molecule to a site other than its active site
Allosteric regulation
381
The phosphate bonds in____ are high energy bonds. Forming phosphate bonds stores energy (Endergonic) Breaking phosphate, bonds releases, energy (exergonic)
ATP
382
This starts with cellular respiration: chemical energy, harvested from fuel molecules in turns into which is the energy used for cellular work, which turns into ADP + P
ATP cycle
383
The reactant of an enzyme acts on is referred to as the enzymes substrate, the substrate binds to an enzyme at its active site the specificity of an enzyme is attributed to the complementary fit between the shape of its active site, the shape of the substrate
Enzymes are specific
384
In a catalyzing reaction an enzyme may use one or more_______________________. Enzymes orient substrate molecules, bring together the atoms that will bond enzymes and stretch the bonds and substrate molecules, making them unstable enzymes contemporary. Add chemical groups to substrates. | things
Mechanism of catalyst
385
Thousands of chemical reactions are occurring in cells simultaneously. The reactions are organized in metabolic pathways. Each reaction is catalyzed by a specific enzyme. The pathways are interconnected. Regulation of enzymes in the reaction rates helps maintain internal homeostasis.
Enzyme Regulation
386
In _______________ a metabolic pathway is halted by the end product binding to the initial enzyme as an inhibitor this allows the cells to produce the necessary amount of product without resources
Feedback inhibition
387
A series of enzyme catalyze reaction, so arrange that the product of one reaction is the substrate of the next
Metabolic pathway
388
The enzymatic breakdown of glucose to pyruvic acid. Occurs in the cytoplasm. ____________ occurs in two phases, energy investment and energy harvest. Inputs: 6C Glucose, 2 NAD+, 2 ATP, 4 ADP + 4 P. Output: 2(3C) pyruvate, 2 NADH, 2 ADP, 4 ATP (produced). Net gain: 2 ATP, 2 Pyruvate+ H2O, 2 NADH + 2H-. Subject to Allosteric regulation. The control point is Phosphofructokinase (allosterically inhibited by ATP)
Glycolysis
389
The ionized form of pyruvic acid, three carbon acid; the end product of glycolysis and the raw material of the citric acid cycle
Pyruvate
390
The catabolic pathways by which electrons are removed from various molecules, and past three intermediate electron carriers to O2, generating H2O and releasing energy; use O2 from the environment and is completely converted into three molecules of CO2
Cellular respiration
391
The anaerobic degradation of a substance such as glucose to smaller molecules, such as lactic acid or alcohol with the extraction of energy; does not involve O2; converts pyruvate into lactic acid or ethyl alcohol (ethanol). The goal is to regenerate NAD+ so that glycolysis can continue to run both pathways yield two ATP per glucose.
Fermentation
392
The compound reacts with oxaloacetate to produce citrate at the beginning of the citric acid cycle; key metabolic intermediate, in the formation of many compounds. Sometimes called acetyl coenzyme A
Acetyl CoA
393
ATP formation in the mitochondrion, associated with the flow of electrons through the respiratory chain
Oxidative phosphorylation
394
The terminal reactions of cellular respiration, in which electrons are passed from NAD or FAD, through a series of intermediate carriers, to molecular oxygen, with a concomitant production of ATP
Respiratory chain
395
An integral membrane protein that couples the transport of proteins with the formation of ATP. Is an enzyme that is embedded in the mitochondrial membrane. Produces ATP from ADP. Uses the flow of protons released in an electron transport chain, protons bind to the root of the __________________ enzyme, changing the rotor shape, causing it to spin.
ATP synthase
396
A force generated across a membrane having two components: a chemical potential (difference in proton concentration), plus an electric potential due to the electrostatic charge on the protein
Protein motive force
397
The formation of ATP in mitochondria and chloroplast, resulting from pumping of protons across the membrane, (against the gradient of electric charge, and of pH), followed by the return of the protons through a protein channel with ATP synthesis activity
Chemiosmosis
398
Metabolic pathway in which glucose is catabolized in the absence of oxygen
Anaerobic respiration
399
Metabolic pathway in which glucose is catabolized in the absence of oxygen with the production of lactic acid
Lactic acid fermentation
400
Metabolic pathway in which glucose is catabolized in the absence of oxygen with the production of ethyl alcohol
Alcoholic fermentation
401
Glucose+ 6 Oxygen ----------> 6 Carbon Dioxide + 6 Water + ATP (Energy). Glucose Oxidizes and is the reducing agent (Loses an Electron) and Oxygen Carbon Dioxide, and Water are Reduced and are the oxidizing agent.
Cellular Respiration Formula
402
ΔG from complete combustion of glucose
-686 kcal/mol
403
Nicotinamide Adenine dinucleotide (____) can oxidize or reduce metabolites. ____ carries electrons between reactions during respiration.
NAD + Electron Shuffle
404
Enzymes transfer a phosphate group form an organic substrate to ADP to create ATP. Least efficient form of ATP production.
Substrate Level Phosphorylation
405
Also called the Kreb’s Cycle, occurs in the mitochondrial matrix. NADH and FADH2 capture electrons from the conversion of acetyl-coA to citric acid. ATP is formed by substrate level phosphorylation. The cycle turns twice for every glucose molecule. Subject to allosteric regulation. Control point is isocitrate dehydrogenase (inhibited by NADH+ H+ and ATP
Citric Acid Cycle
406
Glucose formed from citric acid cycle and glycolysis intermediate. Some intermediates of the citric acid cycle are reactants in pathways that synthesize nucleic acids.
Gluconeogenesis
407
An organism that is capable of living exclusively on inorganic materials, water, and some energy source, such as sunlight, or chemically reduced matter
Autotroph
408
An organism that requires preformed organic molecules as food
Heterotroph
409
The initial phase of photosynthesis, where light energy is converted into chemical energy. Light energy excites the electrons in chlorophyll. The electrons are collected by an electron acceptor and transferred either to an electron transport chain to make ATP or to NADP+ (an electron shuttle). Electrons from the splitting of water replenish those lost from chlorophyll and oxygen is released. ATP and NADPH move to the stroma to be used in the Calvin Cycle.
Light reactions
410
The phase of photosynthesis in which chemical energy captured in the light reactions is used to drive the reduction of CO2 to form, corporal hydrates
Carbon fixation reactions
411
A self propagating wave that travels through space, and has both electrical and magnetic properties
Electromagnetic radiation
412
The distance between successive peaks of wave trains, such as electromagnetic radiation
Wavelength
413
Quantum of visible radiation; "packet " of light energy
Photon
414
As substance that absorbs visible light
Pigment
415
Any of several green pigments associated with chloroplast or certain bacterial membrane; responsible for trapping light energy for photosynthesis
Chlorophyll
416
A graph of light absorption versus wavelength of light; shows how much light is absorbed at each wavelength
Absorption spectrum
417
A graph of a biological process versus light wavelength; shows which wavelengths are involved in the process
Action spectrum
418
In photosynthesis, a group of different molecules that cooperate to absorb light energy and transfer it to a reaction center. Also called antenna system
Light harvesting complexes
419
A group of electron transfer proteins that receive energy from light absorbing pigments and converted to chemical energy by redox reactions
Reaction center of the Photosystem
420
In photosynthesis, the flow of electrons, that forms, ATP, NADPH, and O2
Non-cyclic electron transport
421
Photosynthetic light reactions, the flow of electrons that produces ATP but not NADPH or O2
Cyclic electron transport
422
The mechanism for ATP formation in chloroplast, in which electron transport is coupled to the transport of hydrogen ions (protons, H+) across the Thylakoid membrane
Phosphorylation
423
The stage of photosynthesis in which CO2 reacts with RuBP to form 3PG, 3PG is reduced to a sugar, and RuBP is regenerated, while other products are released to the rest of the plant. Also known as the Calvin Benson cycle.
Calvin cycle
424
Contraction of RuBisCO biphosphate, carboxylase/oxygenase, the enzyme that combines CO2 or O2 with ribulose biphosphate to catalyze the first step of photosynthetic carbon fixation, or Photorespiration, respectively
Rubisco
425
A phosphorated, three carbon sugar; an intermediate and glycolysis and photosynthetic carbon fixation. Also, called Triose phosphate.
Glyceraldehyde 3- phosphate (G3P)
426
An enzyme that catalyzes the addition of oxygen to a substrate from O2
Oxygenase
427
An enzyme that catalyzes the addition of carboxyl functional groups to a substrate
Carboxylase
428
A small opening in the plant epidermis that hermits, gas exchange; bound by a pair of guard cells, whose osmotic status regulates the size of the opening
Stomata
429
The fixation of O2 instead of CO2 in chloroplast, on hot, sunny days, resulting in the release of some CO2 fixed in photosynthesis
Photorespiration
430
Plants produce 3PG as the first stable, product of carbon fixation and photosynthesis, and the ribulose biphosphate as a CO2 receptor
C3 plants
431
Plants that produce oxaloacetate as the first stable product of carbon fixation and photosynthesis and use phosphoenolpyruvate as a CO2 acceptor. C4 plants also perform the reactions of C3 photosynthesis.
C4 Plants
432
Part of a tissue that surrounds the veins of a plant, and where the fixed CO2 is released for the use of the Calvin cycle
Bundles sheath cells
433
The enzyme that combines CO2 with PEP to form four-carbon dicarboxylic acid as the start of C4 photosynthesis, or crassulacean acid metabolism
PEP carboxylase
434
The phosphorlated salt of three carbon acid, formula, OPO3H2 – CO – CO – COOH, that is an intermediate, in metabolic pathway, such as a glycolysis
Phosphoenolpyruvate
435
The metabolic pathway enabling the plants that possess it to store CO2 at night, and then perform photosynthesis during the day with stomata closed
crassulacean acid metabolism (CAM)
436
Organisms that produce glucose through photosynthesis
Producer/Photoautotrophs
437
The ______________ is a way to create ATP using only Photosystem 1.
cyclic flow of electrons
438
The one or more signals required to initiate cell division. The signals may originate from either inside or outside the cell.
Cell division signals
439
Reproduction of a prokaryote by division of a cell into two genetically, identical, progeny cells
Binary fission
440
In bacteria and viruses, the DNA molecule that contains most or all of the genetic information of the cell or virus. And eukaryotes, a structure, composed of DNA and protein, that bears part of the genetic information of the cell.
Chromosome
441
The division of a diploid nucleus to produce haploid cells. The process consist of two successive nuclear divisions of a cell that has undergone only one cycle of chromosome replication. In meiosis one, homologous chromosomes, separate, but retain their sister chromatids. The second division, meiosis two is similar to mitosis in which sister chromatids separate.
Meiosis
442
The stages through which a cell passes between one mitotic division and the next. It includes all stages of interphase (G1, S, and G2 phases), mitosis cytokinesis (M phase).
Cell cycle
443
In the cell cycle, the period between successive nuclear divisions during the chromosomes are diffuse in the nuclear envelope is intact. During ____________, the cell is most active and transcribing and translating genetic information.
Interphase
444
In the cell cycle, the gap between the end of cytokinesis and the onset of S phase. During this phase, each chromosome is a single DNA molecule with associated proteins. Variations in the duration of ___ account for most variability in the length of the cell cycle in different cell types. Some rapidly dividing embryonic cells, dispense with it entirely, whereas other cells may retain and ___ for weeks or even years.
G1 phase
445
In the cell cycle point at which G1 phase ends, and S phase begins
G1 to S transition
446
In the cell cycle, the stage of interphase, during which DNA is replicated. Each chromosome is duplicated and therefore consists of two sister chromatids (the product of DNA replication) the sister chromatids remain joined together until mitosis, when they segregate into two daughters cell
S-phase
447
Each pair of newly replicated, genetically identical (except for mutations) traits
Sister chromatids
448
In the cell cycle, the gap between S phase and the onset of mitosis. The cell makes preparations for mitosis, for example, by synthesize to get assembling the structures that move the chromatids to opposite ends of the dividing cell.
G2 phase
449
A protein Kinase, whose target proteins are involved in transition in the cell cycle, and that is only active when complex with additional protein subunits called cyclins
Cyclin-dependent kinases (CDK)
450
This specific time during G1 phase of the cell cycle, when the cell becomes committed to undergo the rest of the cell cycle
Restriction point (R)
451
A protein that activates, a cyclin-dependent kinase, bringing about transitions in the cell cycle
Cyclins
452
A chemical signal that stimulates cells to grow and divide
Growth factors
453
The nucleic acid protein complex that makes up eukaryotic chromosomes
Chromatin
454
The region, where Sister chromatids join and microtubule attached to chromosomes
Centromere
455
A portion of a eukaryotic, chromosome, consisting of a part of the DNA molecule, is wrapped around a group of histone molecules, and held together by another type of histone molecule. Period of chromosomes is made of many
Nucleosome
456
The major microtubule, organizing center of an animal cell Aynes, a pair of centrioles
Centrosome
457
A paired organelle that helps organize the microtubules in an animal and protest cells during nuclear division
Centrioles
458
Specialized structure on a centromere to which microtubule attached
Kinetochore
459
During mitosis, the separated, chromatids from the beginning of anaphase onward
Daughter chromosomes
460
Formation of new individuals without gamma fusion (and eukaryotes) or conjunction (in prokaryotes). Often takes place by budding or fragmentation.
Asexual reproduction
461
Genetically identical cells, or organisms produced by a common ancestor, by asexual, means to produce many identical copies of DNA sequence by its introduction into, and subsequent a sexual reproduction of, a cell or organism
Clones
462
Reproduction involving the union of gametes
Sexual reproduction
463
Mature sexual reproductive cell: the egg or sperm
Gametes
464
All the cells of the body that are not specialized for reproduction
Somatic cells
465
Pair of mashing chromosomes made up of a chromosome from each of the two sets of chromosomes and a diploid organism
Homologous pairs
466
In cytogenetics, one of a pair (or larger set) of chromosomes, having the same overall genetic composition and sequence. In diploid organisms, each chromosome inherited from one parent is matched by an identical (except for mutational differences) chromosome – it's __________ – from the other parent.
Homologs
467
Having a chromosome complement consisting of one copy of each chromosome; designated 1n or n
Haploid
468
The cell is created by the union of gametes, in which the gamete nuclei is also fused. The earliest stage of diploid generation.
Zygote
469
The union of gametes
Fertilization
470
Having a chromosome complement consisting of two copies (homologs) of each chromosome. Designated 2n
Diploid
471
The highly specific parallel alignment (pairing) of homologous chromosomes during meiosis 1. Homologous chromosomes synapse, and form tetrad or bivalent.
Synapsis
472
An X shaped connection between paired homologous chromosomes in prophase one of meiosis. It is the visible manifestation of crossing over between a homologous chromosomes.
Chiasmata
473
The mechanism by which linked genes undergo recombination. In general, the term refers to the reciprocal exchange of corresponding segments between two homologous chromatids
Crossing over
474
In meiosis, chromatids that has exchanged parts with a non sister homologous chromatid
Recombinant chromatids
475
During meiosis, the random separation of genes carried on non-homologous chromosomes into gametes so that the inheritance of these genes is random. The principle was articulated by Mendel as his second law.
Independent assortment
476
Condition in which one or more chromosomes are either lacking or present in excess
Aneuploidy
477
The failure of sister chromatids to separate in meiosis II or mitosis, or of homologous chromosomes to separate in meiosis 1 result in aneuploidy
Nondisjunction
478
Pertaining to a cell or organism, with only one copy of a chromosome that should be in one (or more) copies
Monosomic
479
The number, forms, and types of chromosomes in a cell
Karyotype
480
Creature, cell death caused by external agents, such as toxins
Necrosis
481
Group of proteases, that catalyze cleavage of target proteins, and are active in apoptosis | C_________
Caspases
482
A disorganized mass of cells. Malignant _________ spread to other parts of the body
Tumors
483
Referring to a tumor that grows to a limited extent, and does not spread to other parts of the body
Benign
484
Referring to a tumor that can grow indefinitely or grow from the original site to other areas of the body
Malignant
485
The spread of tumor cells to region into the body away from the primary tumor
Metastasis
486
A gene that codes for a protein product that stimulates cell proliferation. Mutations in ______________ that result in excessive cell proliferation can cause arise to cancer. | Cancer
Oncogene
487
A gene that code for protein product that inhibits cell proliferation; an active and cancer cells | Cancer
Tumor suppressors
488
Some white cells produce ___________ that promote cell division in other white cells
Interleukins
489
Platelets that initiate blood clotting, stimulate skin cells to divide and heal wounds
Platelet derived growth factor
490
Different variants of a gene
Alleles
491
1. Nucleotides – the building blocks of DNA and RNA 2. Strand – a linear polymer strand of DNA or RNA. 3. Double helix – two strands of DNA. 4. Chromosome – DNA associated with an array of different proteins into a complex structure. 5. Genome – the complete complement of genetic material in an organism.
Levels of DNA structure
492
Stranded, anti-parallel, right, handed helix, sugar, phosphate, backbone, bases on inside, stabilized by each bonding, specific base pairing, about 10 nts per helical turn, grooves are revealed in the space-filling model, The major groove provides a binding cipher proteins that controls the expression of genes, and minor grooves are narrower
Features of DNA
493
In 1953 ________ and __________ proposed the structure of the double DNA helix. They used Linus Pauling method of working out protein structure using simple __________. _________________ x-ray diffraction results were crucial evidence, suggesting the helical structure with uniform diameter.
James Watson; Francis Creek; Ballin stick model; Rosalind Franklin's
494
_____________ said that is DNA based composition from many different species. The results showed the amount of adenine is equal to the amount of thymine, and that the amount of sine is equal to the amount of guanine.
Erwin Chargoff
495
DNA replication produces DNA molecules with one parental strand and one daughter strand; kind of like meiosis
Semiconservative mechanism
496
Do you know replication produces one double helix with both parental strands and the other with two new daughter strands
Conservative mechanism
497
DNA replication produces DNA strands in which segments of new DNA are interspersed with parental DNA
Dispersive mechanism
498
Origin of replication provides an opening, called a replication bubble, that forms _________________________. DNA proceeds outward from these forks. Bacteria have a single origin of replication and eukaryotes have multiple origins of replication.
Replication forks
498
_____________ proteins cut the DNA strands to prevent them from reforming a double helix
Single strand binding
499
______________________ travel slightly ahead of the replication fork, and alleviates coiling caused by the action of Helicase
DNA topoisomerase
500
________________ travels along one DNA strand in the 5'-3' direction and separates the DNA strand
DNA helicase
501
Covalently links nucleotides, DNA synthesizes with the deoxyribonucleic triphosphate, and DNA proofreading
DNA polymerase
502
Free nucleotides with three phosphate groups, breaking covalent bonds to release Pyrophosphate (two phosphate) provide energy to connect nucleotides
Deoxynucleoside triphosphate
503
Requires a primer to get started, DNA primers makes the primer from RNA, the RNA primer is removed and replaced with DNA later, only works in the 5'-3' direction, DNA polymerase is able to covalently link nucleotides together with a primer, which is made by DNA primase
Features of DNA polymerase
504
DNA synthesized as in one molecule, DNA primase makes a single RNA primer, DNA, polymer, and nucleotides in the 5' - 3' direction as it slides forward
Leading strand
505
DNA synthesized 5' - 3' but Okazaki fragments, Okazaki fragments consist of already primers plus DNA
Lagging strand
506
1. DNA wrapping: DNA wrapped around his stones to form nucleosomes, shortens the length of DNA sevenfold. 2. 30-nm fiber: current mod, suggests asymmetric, 3-D zigzag of the nucleus, short length another sevenfold 3. Radio loop domains: interaction between 30 – nm fiber and nuclear matrix, each chromosome located in discrete territory
Levels of DNA compaction
507
Not as compact
Euchromatin
508
Much more compact
Heterochromatin
509
Any agent that increases the mutation rate
Mutagen
510
A change in the genetic material not caused by recombination
Mutations
511
The synthesis of RNA using one strand of DNA as a template. This occurs in three steps. 1. Initiation: RNA polymerase to the promoter. 2. Elongation: the RNA strand grows. 3. Termination: RNA polymerase reaches the terminator and attaches from the DNA initiation
Steps of transcription
512
Transcription factors (collection of proteins: recognizes promoter. A promoter is a unique sequence of nucleotides that signifies the beginning of a gene. The promoter tells RNA polymerase, where to start in which DNA strand to transcribe. RNA polymerase binds to transcription factors and promoters. | This is a step
Initiation
513
Molecular biology, the addition of monomers to make a longer DNA, RNA or protein, during transcription or translation 1. RNA polymerase changes (configuration) shape and is released from the promoter. 2. RNA polymerase reads the DNA in the 5' - 3' direction and adds complementary RNA nucleotides to the growing 3'end of the Arney transcript 3. RNA polymerase doesn't proofread or correct mistakes
Elongation
514
In molecular biology, the end of transcription or translation 1. RNA polymerase reaches the termination sequence (a unique sequence that signifies the end of a gene). 2. RNA polymer dissociates from the DNA and releases the RNA.
Termination
515
The synthesis of a protein (polypeptide). Takes place on ribosomes, using the information and coded with messenger RNA. | The process of
Translation
516
The premise that information flows from DNA to DNA, from DNA to RNA, and from RNA to polypeptide (protein).
The central dogma of molecular biology
517
The transcript, after processing in eukaryotes, of a region of one of the strands of DNA for a gene; it carries information (as a sequence of codons) for the synthesis of one or more proteins | Type of RNA
Messenger RNA (mRNA)
518
A small particle on the cell is a side of protein synthesis
Ribosome
519
Several species of RNA that are incorporated into the ribosome. Involved in peptide bond formation.
Ribosomal RNA (rRNA)
520
A family folded RNA molecules. Each _____ carries a specific amino acid in an anticodon that will pair with the complementary codon in mRNA during translation.
Transfer RNA (tRNA)
521
Production of DNA using an RNA template
Reverse transcription
522
RNA virus that contains several transcriptase. RNA serves as a template for cDNA production, and the cDNA is integrated into a chromosome of the host cell
Retroviruses
523
Enzymes that catalyze the formation of RNA from a nucleic acid (usually DNA) template
RNA polymerase
524
DNA sequence to which RNA polymerase to initiate transcription
Promoter
525
The place within a promoter, where transcription begins
Initiation site
526
In prokaryotes, protein that builds to RNA polymerase, allowing the complex to bind to and stimulate the transcription of a specific class of genes
Sigma factors
527
Proteins that assemble on a eukaryotic chromosome, allowing RNA polymerase II to perform transcription | this is a factor
Transcription factors
528
The initial gene transcript before it is modified to produce functional mRNA. Also knows the primary transcript.
Precursor mRNA or pre-mRNA
529
A portion of a gene within the coding region that is transcribed into pre-mRNA, but is spliced out during mRNA processing, prior to translation
Intron
530
A portion of a gene that is present in mature mRNA. _______ encode polypeptide sequence.
Exons
531
The modification of RNA primary transcript; for example, the splicing out of Intron
RNA processing
532
Methylated, guanine, nucleotide, bonded to the 5' end of the eukaryotic mRNA
5' cap
533
Long sink of nucleotides (100 to 300) added after transcription to the 3' end of most eukaryotic mRNAs
A poly A tail
534
The last stage of RNA processing in eukaryotes, in which the transcripts of Introns are exercised through the action of small nuclear ribonucleoproteins
RNA splicing
535
The list of all possible 64 triplet mRNA sequence (codons) in the amino acids, they encode. Used during translation to convert a linear sequence of nucleotides into a linear sequence of amino acids.
Genetic code
536
The three nucleotides in messenger, RNA, that direct the placement of a particular amino acid into a polypeptide chain
Codon
537
The mRNA triplet (AUG) that acts as a signal for the beginning of translation at the ribosome
Start codon
538
Any of the 3MRNA codons that signal, the end of protein translation at the ribosome: UAG, UGA, UAA. Also called a nonsense codon.
Stop codons
539
The nucleotides that transfer RNA that pair with a complementary triplet (a codon) in messenger RNA
Anticodon
540
The genetic code is ___________ meaning that all organisms use the codes the same way with only few variations, _____________ (Redundant). Most amino acids are encoded by two or more codons, and _____________ where codon codes for one amino acid
Universal; degenerate: unambiguous
541
the ribosome binds to mRNA, “start” tRNA (UAC) binds to “start” codon (AUG) of mRNA at the P site of the ribosome. In prokaryotes rRNA binds to mRNA recognition site “upstream” from start codon. In eukaryotes the small subunit binds to the 5′ cap on the mRNA and moves until it reaches the start codon.
Translation Initiation
542
The polypeptide grows by binding of complementary tRNAs, and movement of tRNAs from A site to P site. Large subunit catalyzes two reactions: It breaks bond between tRNA in P site and its amino acid. Peptide bond forms between that amino acid and the amino acid on tRNA in the A site
Translation Elongation
543
The ribosome reads the “stop” codon on the mRNA, a release factor binds to the stop codon and releases the polypeptide and dissociates the ribosome.
Translation Termination
544
______________ binds to the stop codon and releases the polypeptide and dissociates the ribosome.
release factor
545
The DNA molecule made in the laboratory that is derived from two or more genetic sources
Recombinant DNA
546
On a piece of two stranded DNA, short, complementary, single-stranded regions produced by the action of a restrictive endonuclease, _______________ facilitate the joining of segments of DNA from different sources.
Sticky end
547
To produce many identical, copies of a DNA sequence by its introduction into, a subsequent asexual reproduction, of a cell or organism
Clone
548
Insertion of recombinant DNA into a host (animal) cell
Transformation/transfection
549
Containing DNA incorporated into the genetic material
Transgenic
550
A gene, such as one encoding resistance to an antibiotic, that can be used to identify (select cells that contain recombinant DNA among a large population of untransformed cells,
Selectable marker
551
Stem cells that have the capacity to develop into any other type of cell
Totipotent stem cells
552
A region of DNA replicated from a single origin of replication
Replicon
553
A plasma or virus that carries an inserted piece of DNA into the bacterium or cloning purposes, incompetent DNA technology.
Vector
554
A genetic marker whose expression is easily observed; often included in recombinant DNA to indicate the presence of the recombinant DNA in a host cell
Reporter gene
555
All of the clown, DNA fragments generated by the breakdown of genomic DNA into smaller segments
Genomic library
556
DNA formed by reverse transcript acting with an RNA template; an essential intermediate in the reproduction of retrovirus; used as a tool in recombinant DNA technology; lacks introns
Complementary DNA (cDNA)
557
An enzyme that catalyzes, the production of DNA (DNA), using RNA as a template; essential to the reproduction of retroviruses
Reverse transcriptase
558
A collection of contemporary DNA derived from mRNA of a particular tissue at a particular time in the lifecycle of an organism
cDNA library
559
Laboratory manipulation of RNA in which the RNA is first incubated with reverse transcript (RT) to make c DNA, and then the cDNA amplified for analysis by the polymerase chain reaction (PCR)
RT – PCR
560
A single stranded, RNA molecule, complementary to, and thus targeted against, and mRNA of interest to block its translation
Antisense RNA
561
In some prokaryotes, clustered regularly interspace, short palindromic repeat sequences in DNA that are adjacent to sequences from invading viruses. When the two sequences are transcribed, the RNA binds a complementary RNA, that binds a nucleus, CAS9, that can Cleve and inactivate the genome of the invading virus. This mechanism can be adapted for inactivating and mutating any gene.
CRISPR
562
Do use of living cells or organisms to produce materials, useful to humans
Biotechnology
563
A DNA vector, such as a plasma, with a DNA sequence that allows for the expression of an inserted gene into mRNA, and a protein in a host cell
Expression vectors
564
The use of genetically modified animals or plants to reduce medically useful products
Pharming
565
The promoter DNA sequence is called
TATA box
566
_____________ and ________________ are used to cut DNA into fragments and then splice them together in new combinations. ___________________ cleave DNA at palindromic recognition sequences (restriction sites).
Restriction enzymes; DNA ligase
567
______________________ (e.g. E. coli, mice, fruit flies, etc.) are often used in the laboratory in an effort to understand the function of a particular gene. Transgenics can also be created to solve a particular problem (ie. disease) or to produce a pharmaceutical product.
Transgenic model organisms
568
Human insulin (the majority of insulin on the market) is produced in transgenic
E. coli, yeast and now safflower plants.
569
Vitamin A deficiency is extremely common in the developing world especially in areas plagued by famine. Vitamin A deficiency can cause blindness, death, and poor outcomes during pregnancy. Rice plants does not naturally produce vitamin A but is a staple food crop for much of the world’s population. _________ has been genetically modified to produce a precursor of vitamin A in the endosperm using a gene from corn.
Golden Rice
570
This modified salmon was originally engineered in 1989 but has recently received approval by the FDA to be commercially produced and sold. The Chinook Salmon has been modified with a gene from an eel-like fish called an Ocean Pout. This has created a fish that can reach market size in 16-18 months instead of 3 years.
AquAdvantage Salmon
571
This system can be used in gene editing by supplying donor DNA that can replace mutated DNA. The Cas protein is supplied with a “guide RNA”. When it reaches a section of the genome that is complementary to this guide. It makes a cut. This cut is repaired by the cell’s own DNA repair mechanisms which replace the cut with a replacement DNA molecule.
CRISPR-Cas9 Gene Editing System
572
A chinese scientist, He Jiankui, has announced that he has edited the genomes of human embryos in the fall of 2018. These embryos were implanted and the children were born with no known side effects. Dr. Jiankui used the Crispr-Cas 9 genome editing system to modify a gene called CCR5. Variants in the CCR5 gene have been known to cause resistance to HIV. The scientific community responded with shock and outrage. Why?
First Children Born With Edited Genomes
573
Covid-19 is an RNA virus (meaning its genome is entirely RNA). In order to detect Covid-19 a physician will take a swab and laboratory will perform an RNA extraction from the sample. A reverse transcriptase will be used to reverse transcribe any RNA in the sample to DNA. A PCR (polymerase chain reaction) will be run with this DNA sample and short DNA fragments designed to be complementary to DNA sequence only found in Covid 19.
Covid-19 RT PCR Test
574