ex 2: CARE & USE OF MICROSCOPE Flashcards

1
Q

brightfield light microscopy

A

binocular compound microscopes, meaning they have 2 eyepieces and more than one lens

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2
Q

ocular lenses

A

(eye pieces) that magnify the specimen 10x

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3
Q

objective lenses

A

magnify 4X, 10X, 40X, 100X

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4
Q

condenser lens

A

does not magnify but instead focuses the light

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5
Q

objective lens are located on

A

rotating nosepiece

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6
Q

rotating nosepiece

A

allows user to switch each easily from one magnifying lens to another

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7
Q

4X objective is known as

A

the scanning lens

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8
Q

10X objective is known as

A

the low power lens

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9
Q

40X objective is known as

A

high-dry lens

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10
Q

100X objective is known as

A

oil immersion lens

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11
Q

path of light is from the

A

illuminator (light source)

up through the condenser lens

throught the specimen on the slide

up through the objective lens

to the ocular lens

finally to the person viewing the microscope

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12
Q

light intensity knob (dimmer)

A

control the amount of light from the illuminator

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13
Q

iris diaphragm

A

can be opened and closed to control the amount of light that passes from the illuminator through the condenser lens

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14
Q

stage

A

the flat surface upon which a slide is placed

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15
Q

course focus knob

A

moves the stage vertically, toward, and away from the objective lens

should only be used for lower power objectives (4X and 10X)

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16
Q

fine focus knobs

A

move the stage up and down from the objective lens

17
Q

parfocal

A

when a specimen in focus at one magnification it will