Exam 1: RBC/WBC eval Flashcards
(19 cards)
Normal dog PCV
35-50%
PCV (Packed Cell Vol)
% of whole blood that is erythrocytes aka hematocrit (HCT) (calculated value on CBC)
Buffy coat
- After spun down, white band above RBC’s
- WBC’s, platelets, nucleated erythrocytes
Plasma
-After spun down, layer above buffy coat
Yellow plasma
- Normal or Icteric (due to increased bilirubin concentration from heme breakdown in the blood )
- LA - icteric or carotene pigment due to diet
Lipemic plasma (white)
- Excess of chylomicrons (lipemia)
- Postprandial collection or lipid metabolism dz
Red plasma
- Hemolysis causing hgb presence in plasma
- In-vtiro causes (technique, or lipemia presence)
- In-vivo causes (hemolytic anemia)
Total Protein
- Refractometer estimates protein concentration in plasma
- assumes all other plasma solutes are normal. If abnormal, TP increased falsely
- Measure Albumin & globulin on chem to check
Normal TP in dogs
6.5 - 8 gm/dl
What can artificially increase TP?
- increased Albumin, globulin
- urea, glu, cholesterol (negligible effect)
- Lipemia
- severe hemolysis
In dehydration, what happens to albumin?
increases
likely to see increased TP as well
Increased PCV, increased TP indicates what?
dehydration
Decreased PCV, decreased TP indicates what?
blood loss
WNL PCV, decreased TP indicates what?
decreased prot production or
increased prot loss
WNL PCV, increased TP indicates what?
increased globulin production
dehydration in an anemic patient
Decreased PCV, WNL TP indicates what?
chronic disease anemia
hemolysis
Increased PCV, WNL TP indicates what?
Dehydration in low TP animal
Polycythemia
Convert TNCC (total nucleated cell count) to absolute concentration
% cell type x TNCC = cell type/ul (more useful number)
How many platelets should you see per 100x field?
8-12
