Exam 2 Flashcards
the percentage of the surface of a culture dish that is covered by adherent cells
confluence
transferring cells to a new vessel with fresh growth medium to provide more room for continued growth.
subculturing
After the first subculture, the primary culture becomes known as a:
cell line/subclone
bipolar or multipolar, have elongated shapes, and grow attached to a substrate
Fibroblast-like cells
polygonal in shape with more regular dimensions, and grow attached to a substrate in discrete patches
Epithelial-like cells
spherical in shape and usually grown in suspension without attaching to a surface
Lymphoblast-like cells
monolayers on an artificial substrate
adherent
free-floating in the culture medium
Suspension
Generally used to grow large batches
Contain filtered caps to allow CO2 exchange
Flasks
Used for smaller batches
Individual control over the wells
Well plates
Generally used with bacteria culturing
Often solid medium is used
Petri dishes
Media/medium - 4 components
- Amino acids
- Inorganic salts
- Vitamins
- Sometimes antibiotics
Serum - 3 components
- Macromolecules
- Lipids
- Growth factors
Most normal mammalian cell lines grow well at this pH
7.4
Most mammalian cells grow best at:
37°C
Humid CO2 incubators control these 3 conditions
- CO2
- Humidity
- Temperature
provides an aseptic work area while allowing the containment of infectious splashes or aerosols generated by many microbiological procedures.
laminar flow hood
only protect the cultures from the worker and used if the cultures are harmless to you and the environment
clean benches
generally used to protect the cultures from workers and workers from the cultures
Biological safety cabinets (BSCs)
Sterile/aseptic techniques
-Wear gloves
-Spray everything with ethanol
-Keep hands inside hood
-Change pipette tips for new materials
-Don’t pour reagents directly from bottles/flasks
-Don’t uncover anything until its ready for use
the most common type of brain tumor
glioblastoma
Usually has poor prognosis and high mortality for glioblastoma due to:
its highly invasive behavior
Lab 5 Reagents (Changing media)
-DMEM
-FBS
-Penicillin-Streptomycin