Exam 3 Flashcards
(50 cards)
Monomers of DNA
Adenine, Thymine, Cytosine, Guanine (ATCG)
Shape of DNA
Double helix
Nitrogenous bases found in DNA
Adenine, Thymine, Cytosine, Guanine
A=T G=C
Bonds holding DNA together
Hydrogen bonds
What makes up a nucleotide?
Five carbon sugar, phosphate group, nitrogenous base
What is the purpose of DNA replication?
Prepare for cell division by creating a copy of the genome
What is semi-conservative?
One original strand and one new strand
What are the copied chromosomes held together by?
Centromere
Polymerization happens via the
dehydration reactions
DNA polymerase works in
only one direction
Combines monomers to form polymers
Anabolic reaction
Requires energy
Endergonic reactions
DNA replication in what direction?
5’ to 3’ direction
Prokaryotes have how many origins of replication?
One origin
Eukaryotes have how many origins of replication?
Many origins
DNA unwinds from the origin of replication to form a
replication bubble
Each origin of replication has
two replication forks
What is replisome?
Molecular machine that does replication
How many replisomes for each origin of replication?
Two
What is an FNA primer?
Starting nucleotide sequence; gives DNA polymerase a place to start
What is DNA polymerase? DNA polymerase?
Main replication enzymes; Adds nucleotides bases in 5’ to 3’ direction (always add to 3’ -OH)
Leading strand is built
continuously towards replication fork
Lagging strand is built
discontinuously (in section) away from the replication fork
What are the sections of the lagging strand called?
Okazaki fragments